Mitochondrial Gene Expression of Three Different Dragonflies Under the Stress of Chlorpyrifos

Insects, Journal Year: 2025, Volume and Issue: 16(1), P. 85 - 85

Published: Jan. 16, 2025

Chlorpyrifos (CPF) is an organophosphate insecticide that extensively utilized globally due to its effectiveness against over 200 pest species. CPF exhibits toxicity primarily through the inhibition of acetylcholinesterase (AChE) enzyme, while mitochondrial damage and dysfunction have also been observed. The present study quantified transcript levels mitochondria protein-coding genes (mtPCGs) using quantitative real-time polymerase chain reaction (RT-qPCR) in samples larvae three dragonfly species (A. parthenope, E. elegans, G. confluens) under different stress. By exposing from uncontaminated populations 0.05 μg/L for 24 h, seven mtPCGs A. parthenope were significantly increased (p < 0.05) by 1.89 ± 0.42-fold COI, 4.30 0.24-fold COIII, 5.94 0.17-fold ND1, 4.69 0.56-fold ND2, 3.44 0.48-fold ND4, 2.19 0.53-fold ND4L, 5.05 0.36-fold Cytb, respectively. In ND4 1.23 0.15, 1.48 0.31, 1.98 0.25-fold, respectively 0.05). confluens, 1.56 0.13, 1.50 0.26, 3.74 0.40-fold, 0.01). It was demonstrated showed significant up-regulation stress absence mortality. all species, indicating it important target gene. underscores response gene expression pollutants, pesticide influences can potentially alter act as a method assessing aquatic ecosystem health.

Language: Английский

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A Genome-Wide Analysis of Nuclear Mitochondrial DNA Sequences (NUMTs) in Chrysomelidae Species (Coleoptera)

Insects, Journal Year: 2025, Volume and Issue: 16(2), P. 150 - 150

Published: Feb. 2, 2025

Nuclear mitochondrial DNA sequences (NUMTs) are fragments in the nuclear genome, and their unclear distribution Chrysomelidae species hinders selection of accurate molecular markers for identification phylogenetic analysis. Our study presents a genome-wide survey NUMTs 32 species. Filtering strategies based on sequence length open reading frame (ORF) features were employed to identify protein-coding genes (PCGs) minimally affected by NUMTs. Phylogenetic relationships inferred from both PCG datasets COX1 dataset containing results show that chromosomally specific, species-specific, widely distributed. ATP8, COX1, ND1, ND4 identified as relatively reliable markers. analysis is influenced other factors such type saturation. A total 66 independent gene integration events estimated across species, mostly distinct lineages. These findings suggest reflect key evolutionary processes flow lineage diversification. Their prevalence emphasizes need refined analysis, while also highlighting importance understanding contribution species’ history.

Language: Английский

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Morphology of larvae and pupae of four species of Neotropical Acanthocinini (Cerambycidae, Lamiinae)

Zootaxa, Journal Year: 2025, Volume and Issue: 5590(3), P. 325 - 344

Published: Feb. 23, 2025

Acanthocinini is a tribe of Lamiinae with questionable monophyly and close affinity Acrocinini Colobotheini. Despite being the largest number known immature forms, it poorly characterized, only by arbitrary features adults. Here we describe illustrate larva pupa four species Acanthocinini: Baryssinus marisae Martins & Monné, 1974, Oedopeza umbrosa (Germar, 1823), Oxathres sparsa Melzer, 1927 (based on exuviae) Xylergatoides asper (Bates, 1864), all but O. umbrosa, representing first description their genera.

Language: Английский

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Assembly and analysis of the complete mitochondrial genome of Leonurus japonicus (Lamiaceae)

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 18, 2025

Language: Английский

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Phylogenetic and biogeographic reconstruction of Lamiini sensu lato (Coleoptera, Cerambycidae) reveals South Gondwanan origin and Panthalasso-Tethyan dispersal pathways

Biosystems Diversity, Journal Year: 2025, Volume and Issue: 33(1), P. e2510 - e2510

Published: March 11, 2025

In this study I conduct the phylogenetic and biogeographical analysis of Lamiini sensu novo (Coleoptera, Cerambycidae, Lamiinae), confirming it as a monophyletic group that is broader than previously assumed supporting redefinition unified tribe – novo. The consist eight major clades (TAENI, DORCA, ACALO, BATOC, PSACO, ANOLPL, MONOC, LAMIA) integrates together several traditionally recognized tribes (Dorcaschematini, Batocerini, Petrognathini, Rhodopini, Monochamini, Dorcadionini, Phrissomini). Most them, including Phrissomini are polyphyletic, representing multiple lineages with intricate evolutionary history. Phylogeographic suggests South Gondwanan origin s. l., its ancestral lineage (LaCA) emerging in Antarctica–South America suture zone before global expansion through three key migration routes: northeast Pantalassic, Trans-Tethyan, southwest Pantalassic. Continental drift climate oscillations influenced process. Initial diversification (~70–60 Ma) led to vicariant taxa due continental isolation, basal ACALO) distributed across America, North Australia. Extinction events, K–T boundary (~66 Cenozoic glaciations, further shaped diversification. rise BATOC PSACO marked early crown-group l. within island continent Greater India archipelagos Trans-Tethyan Arc (~60–50 Ma). collision Asia (~50–45 facilitated faunal exchanges, aiding dispersal into Africa Southeast driving ANOPL (45–35 ACALO clade underwent secondary during initial Australia Eurasia (~25–20 Ma), coinciding uplift New Guinea Miocene. LAMIA likely originated East During Miocene Climatic Optimum, expanded via Gomphotherium Land Bridge (~16 colonized Europe. Later cooling aridification (~5–11 drove diversification, particularly Eurasian steppes. MONOC also Asia, adapting montane coniferous forests cooling. It spread glaciations (~20 Ma, ~14 entered Beringia. Expansion Mesoamerica, northern Caribbean occurred Pleistocene (~0.01–2 when fluctuating sea levels enabled dispersal. summary, findings refine phylogeny highlight impact geological climatic events on evolution. This clarifies taxonomic ambiguities provides framework for future research biogeographic patterns.

Language: Английский

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Comparative Mitogenome of Phylogenetic Relationships and Divergence Time Analysis within Potamanthidae (Insecta: Ephemeroptera)

Insects, Journal Year: 2024, Volume and Issue: 15(5), P. 357 - 357

Published: May 15, 2024

Potamanthidae belongs to the superfamily Ephemeroidea but has no complete mt genome released in NCBI (except for two unchecked and one partial genome). Since sister clade always been controversial, we sequenced seven genomes of (two species from Rhoenanthus five Potamanthus) order rebuild phylogenetic relationships this study. The divergence time was also investigated by utilizing fossil calibration points because indeterminate origin time. In addition, coreanus Potamanthus luteus are low-temperature environments, aimed explore whether these were under positive selection at level. Amongst 13 PCGs, CGA used as start codon COX1, whereas other genes conformed initiating with an ATN codon. From analysis, UUA (L), AUU (I), UUU (F) had highest usage. Furthermore, DHU arm absent secondary structure S1 all species. By combining PCGs 2 rRNAs, reconstructed relationship within Ephemeroptera. monophyly supported results. + (Ephemeridae Polymitarcyidae) recovered a high prior probability. times traced be 90.44 Mya (95% HPD, 62.80–121.74 Mya), originated approximately 64.77 43.82–88.68 thus belonging late Pliocene Epoch or early Miocene Epoch. data indicated that R. negative ATP8 ND2 evolutionary rate.

Language: Английский

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Insights into the phylogeny of longhorn beetles from phylogenomic data

Zoological Journal of the Linnean Society, Journal Year: 2024, Volume and Issue: 203(1)

Published: Dec. 13, 2024

Abstract Cerambycidae (Coleoptera: Chrysomeloidea) are a widely distributed group of insects with significant economic importance. Despite substantial efforts and some advancements in inferring the phylogeny Cerambycidae, high-level phylogenetic relationships within family, including subfamily tribe-level classification, remain contentious. In this study, we performed whole-genome sequencing on total 65 species. We integrated these comprehensive genomic data existing transcriptome to conduct analysis s.s. The results supported monophyly Lamiinae, Cerambycinae, Spondylidinae, whereas Lepturinae Prioninae were found be non-monophyletic. Lamiinae recovered as sister all other Spondylidinae clade comprising Necydalinae, Cerambycinae identified consisting Parandrinae. Within tribes Mesosini, Saperdini, Dorcaschematini monophyletic; however, Acanthocinini, Agapanthiini, Pogonocherini, Pteropliini, Lamiini, Monochamini This study classification integrating current into Lamiini. Molecular dating suggests that diversification began at boundary between Jurassic Cretaceous periods, ~145 Mya.

Language: Английский

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Complete mitochondrial genome of Perilla frutescens (Lamiaceae) insights into structure, Codon usage, repeats, and RNA editing

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: March 18, 2024

Perilla frutescens (L.) Britton, a member of the Lamiaceae family, is versatile plant highly valued for its unique aroma and medicinal properties. Additionally, P. seeds are rich in α-linolenic acid, holding significant economic importance. While nuclear chloroplast genomes have already been documented, complete Mitochondrial genome sequence has yet to be reported. In this investigation, we conducted sequencing, annotation, assembly entire using combination Illumina PacBio data. The resulting assembled spans 299,551 bp exhibits typical circular structure, with GC content 45.23%. Within genome, total 59 genes were identified, encompassing 37 protein-coding genes, 20 tRNA 2 rRNA 18 introns present 8 genes. Notably, codons display notable A/T bias. Our analysis also revealed 293 dispersed repeat sequences, 77 simple repeats (SSRs), 6 tandem sequences. RNA editing sites exhibited preference formation leucine at amino acid sites. Furthermore, identified 70 fragments (12,680 bp) that transferred from accounting 4.23% genome. Phylogenetic indicated among plants, most closely related Salvia miltiorrhiza Platostoma chinense. Inter-species Ka/Ks results suggested <1 28 PCGs, indicating these will continue evolve under purifying selection pressure. findings study contribute enrichment data provide theoretical foundation future molecular breeding research on frutescens.

Language: Английский

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Comparative Mitogenomics in the Genus Chlorophorus (Coleoptera: Cerambycidae) and Its Phylogenetic Implications

Insects, Journal Year: 2024, Volume and Issue: 16(1), P. 8 - 8

Published: Dec. 27, 2024

Chlorophorus Chevrolat, 1863, one of the most species-rich genera Clytini, comprises 36 subgenera and 302 species/subspecies, with some species being significant economic importance. To assess monophyly subgeneric system this genus, we newly obtained mitochondrial genomic data from 21 via high-throughput sequencing reconstructed phylogeny genus using ML BI methods. The genomes all sequenced were found to comprise 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), two ribosomal (rRNAs), non-coding region (control region, CR), reflecting a highly conserved gene arrangement. range 15,387 bp 15,779 in length, exhibited AT bias. Analysis non-synonymous synonymous substitution rates revealed that ATP8 has highest evolution rate, whereas COI lowest. Further, phylogenetic analyses performed based on different datasets (13 PCGs + 2 rRNAs, rRNAs tRNAs, PCG123, PCG12) maximum likelihood (ML) Bayesian inference (BI) results indicate is monophyletic, but Humeromaculatus Özdikmen, 2011; Immaculatoides 2022; Brevenotatus (s. str.) are not monophyletic. Based comparison male genitalia, sclerites endophallus may exhibit signals for genus.

Language: Английский

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