Informed consent in assisted reproductive technology: Implications for pediatric clinicians DOI
Mary Elaine Graham, Shannon M. Blee, Rebecca D. Pentz

et al.

Developmental Medicine & Child Neurology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 17, 2024

After conceiving through assisted reproductive technologies (ART), parents may present to their pediatrician with concerns related child's neurodevelopment, including whether health be use of ART. Pediatricians unfamiliar the ART process and what families endured up this point, resulting in difficulty counseling these discussions. Before presentation pediatrician, have undergone extensive evaluation endocrinologists. During counseling, endocrinologist provides information on maternal childhood risks associated However, rapidly evolving field, providing comprehensive, patient-centered, informed consent is increasingly complex patients properly can challenging. When gone proper process, when has an understanding entails, care child optimized. In review, we discuss complexities prenatal that navigate before presenting pediatricians. We emphasize importance discussions highlight ethical principles, as well emotional, medical, legal, financial stressors face during ART, belief will improve pediatricians subsequently provide.

Language: Английский

PGT-A: what’s it for, what’s wrong? DOI
Stéphane Viville, Mohamed Aboulghar

Journal of Assisted Reproduction and Genetics, Journal Year: 2025, Volume and Issue: 42(1), P. 63 - 69

Published: Jan. 1, 2025

Language: Английский

Citations

1
Trophectoderm Biopsy: Present State of the Art DOI Open Access
A. De Vos, Neelke De Munck

Genes, Journal Year: 2025, Volume and Issue: 16(2), P. 134 - 134

Published: Jan. 24, 2025

Trophectoderm (TE) biopsy is at present the most widely used procedure for preimplantation genetic testing (PGT). At blastocyst stage, more TE cells (five to seven) can be obtained analysis. While removing and not touching inner cell mass (ICM), less invasive. Due a natural selection happening between day 3 5, 6 or 7 of human embryo development, fewer embryos will have biopsied tested. An additional benefit, especially in view aneuploidy (PGT-A), lower level mosaicism stage. The involves two steps: laser-assisted zona pellucida (ZP) opening excision five eight from with without laser energy. Different protocols emerged over time variations regarding technique, exact moment ZP opening, method removal. ‘pulling’ excision, whereas ‘flicking’ represents mechanical approach assistance. Embryo developmental speed reaching full/expanded hatching/hatched stage dictates timing procedure, mostly on 5 post-insemination, lesser extent even 7. inclusion quality delayed blastocysts may impact sample as well clinical outcome. Intracytoplasmic sperm injection (ICSI) still preferred fertilization PGT-M (monogenic disorders) PGT-SR (structural rearrangements). However, conventional vitro (IVF) seems feasible PGT-A (aneuploidy testing). In absence (conclusive) result, re-biopsy cryopreserved possible, however, reduced outcomes. So far, neonatal outcome post-TE has so far been reassuringly documented.

Language: Английский

Citations

0
Navigating the practical challenges and ethical dilemmas of surplus cryopreserved human embryos DOI
George Anifandis,

Dimitrios Ioannou,

Georgia Kokkali

et al.

Systems Biology in Reproductive Medicine, Journal Year: 2025, Volume and Issue: 71(1)

Published: Jan. 28, 2025

Cryopreservation, the use of very low temperatures to preserve structurally intact living cells and tissues, has seen exponential growth in field vitro fertilization (IVF). In last decade, cryopreservation embryos freeze-all protocols have become an essential aspect a prerequisite for successful IVF outcome. Moreover, vitrification, which is fast safe method, proved be effective choice cryopreserving gametes embryos. The increasing number cryopreserved worldwide cryobanks clinics undisputable fact that raises important physiological, ethical, moral considerations merit careful examination discussion. Many couples utilizing assisted reproduction will surplus embryos, other words they already completed their family without exhausting all were created during process. Additionally, global market also experienced significant due various factors, including advancements technology, increased awareness about infertility treatments, changing societal norms towards delayed parenthood. Thus, foreseeable future phenomenon likely remain unresolved. present review, following description method physiological changes bioethical issues raised by discussed alongside possible solutions resolving this phenomenon.

Language: Английский

Citations

0
Maternal age is associated with apoptotic gene abundance patterns in blastocoel fluid-conditioned media from euploid embryos: a pilot study DOI Creative Commons

Dieu Thao Nguyen,

Hannah Archer,

Angel Earle

et al.

Journal of Assisted Reproduction and Genetics, Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Abstract Purpose This retrospective study measured global gene abundance using RNASeq of blastocoel fluid-conditioned media from euploid ICSI-generated embryos to identify genes and signaling pathways associated with maternal age. Methods Blastocoel was obtained following trophectoderm biopsy day-5 blastocysts. Media for were 24 blastocysts (9 patients aged 35 or older). Transcriptome analysis identified differentially expressed when comparing advanced age those younger than 35. Further on the conducted another group samples RT-qPCR. Results Twenty-five protein encoding in under Genes proteins SHARPIN BCL2L12 showed a statistically significant increase ( p < 0.05) Abundance RT-qPCR additional revealed elevated successful implantation alongside decrease CASP8 abundance. pattern opposite Conclusions uncovered differential apoptotic by assessing These unique patterns may provide insight into regulation apoptosis women age, how this pathway impact outcomes.

Language: Английский

Citations

0
Re-biopsy and modified biopsy in mosaic human embryos with simple segmental deletion DOI
Weizhou Wang, Bin Liu, Zhi-Ping Jin

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Abstract Background: There is a considerable proportion of false positive when the result preimplantation genetic testing for aneuploidy chromosomal mosaicism. To assess concordance among clinical trophectoderm biopsy, second and inner cell mass biopsy in mosaic human embryos with segmental deletion evaluate modified protocol. Methods: This retrospective study was included 102 pretested blastocysts, which were classified as mosaics one to two chromosomes affected, donated by 84 couples single, high-volume fertility center Beijing China. Re-biopsy taken from symmetrical sites near each embryo. Samples prepared protocol, isolated portions into single cells, cells normal morphology collected debris removed. samples analyzed next-generation sequencing. Main outcome measures between blastocyst, mosaicism Results: Only six (6.4%) 94 presented concordant aberrations, four (4.2%) de novo abnormalities, (89.4%) did not show any abnormalities. For trophectodermsamples, 64 (67.4%) 95 biopsies 78 (80.4%) 97 or duplication. The protocol associated fewer abnormalities (19.6% versus 32.6%; odds ratio: 0.503, 95% confidence interval: 0.26 0.973) (10.3% 23.2%; 0.381, 0.17 0.857) compared biopsy. Conclusions: A embryo simple extremely low predictive Removal could reduce incidence mosaics. Clinical trial registration:not applicable.

Language: Английский

Citations

0
Aligning genotyping and copy number data in single trophectoderm biopsies for aneuploidy prediction: uncovering incomplete concordance DOI Creative Commons
Lisa De Witte, Machteld Baetens, Kelly Tilleman

et al.

Human Reproduction Open, Journal Year: 2024, Volume and Issue: 2024(4)

Published: Jan. 1, 2024

To what extent can genotype analysis aid in the classification of (mosaic) aneuploid embryos diagnosed through copy number a trophectoderm (TE) biopsy?

Language: Английский

Citations

2
Initially categorized 46,XY embryo transfer ending with 45,X products of conception—a case report and a review of discordant result management DOI Creative Commons
Prapti Singh,

Alyssa Snider,

Refik Kayalı

et al.

F&S Reports, Journal Year: 2024, Volume and Issue: 5(3), P. 328 - 332

Published: May 22, 2024

To report a case of an initially categorized euploid male embryo screened using preimplantation genetic testing (PGT) resulting in miscarriage and products conception consistent with Turner syndrome, to discuss additional workup considerations cases discrepancy.

Language: Английский

Citations

0
PGT-A: Yes versus No DOI Open Access

Nehal Adel,

Hisham Saleh

Journal of Reproductive Medicine and Embryology, Journal Year: 2024, Volume and Issue: 0(0), P. 0 - 0

Published: July 29, 2024

The goal of In Vitro Fertilisation is a healthy full-term fetus. Still ART remains inefficient. selection embryos for transfer based mainly on morphology. Morphology alone cannot select the embryo with highest implantation potential. About 60% early human are aneuploid, and it increases dramatically increasing maternal age.Embryo aneuploidy leading cause failure miscarriage after IVF.Aneuploidy screening through taking biopsy from morphologically normal & analysis DNA allows to deselect abnormal chromosomal number. Aneuploid unable self-correct. proposed benefit improve over morphological assessment enhance likelihood that transferred would result in live birth.Despite its worldwide use, PGT-A has faced many challenges concerns opponents regarding value, accuracy, validity, cost effectiveness. Several fundamental questions must be answered this regard.

Language: Английский

Citations

0
Informed consent in assisted reproductive technology: Implications for pediatric clinicians DOI
Mary Elaine Graham, Shannon M. Blee, Rebecca D. Pentz

et al.

Developmental Medicine & Child Neurology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 17, 2024

After conceiving through assisted reproductive technologies (ART), parents may present to their pediatrician with concerns related child's neurodevelopment, including whether health be use of ART. Pediatricians unfamiliar the ART process and what families endured up this point, resulting in difficulty counseling these discussions. Before presentation pediatrician, have undergone extensive evaluation endocrinologists. During counseling, endocrinologist provides information on maternal childhood risks associated However, rapidly evolving field, providing comprehensive, patient-centered, informed consent is increasingly complex patients properly can challenging. When gone proper process, when has an understanding entails, care child optimized. In review, we discuss complexities prenatal that navigate before presenting pediatricians. We emphasize importance discussions highlight ethical principles, as well emotional, medical, legal, financial stressors face during ART, belief will improve pediatricians subsequently provide.

Language: Английский

Citations

0