POLM inhibits porcine epidemic diarrhea virus replication by degrading multiple viral structural proteins

Journal of Virology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

Porcine epidemic diarrhea, as a porcine diarrhea virus (PEDV)-induced infectious intestinal condition typified by emesis, dehydration, and anorexia, leads to death rates high 100% among suckling piglets. Given the existing commercial vaccines, it is essential study host-virus interactions formulate efficient anti-viral regimes. This concerned host factor POLM (a DNA polymerase family member) that exerts an effect against PEDV proliferation. Our results indicated expression was increased following infection regulated transcription FOXA1. In addition, our findings targeted degraded structural proteins (N, S2, M) autophagy pathway inhibit could recruit E3 ubiquitination ligase MARCH8 for N, M protein ubiquitination, which subsequently recognized p62, cargo receptor, translocation autophagic lysosome, therefore degrading preventing summary, we showed novel therapeutic target combating PEDV, i.e., using POLM-MARCH8-p62-autophagosome degrade proteins.IMPORTANCEPEDV coronavirus causes mortality in piglets, poses significant economic damage swine farming. During infection, cells may promote natural immune response suppress viral replication through variety of potential factors. this study, found upregulation FOXA1 factor) during infection. It be new replication, interacted with (an ubiquitin ligase) p62 receptor) facilitate degradation via process. Apart from elucidating previously unidentified function POLM, also provides perspective studying factors act regulators anti-PEDV pathways.

Language: Английский

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Porcine epidemic diarrhea virus induces mitophagy to inhibit the apoptosis and activation of JAK/STAT1 pathway

Veterinary Microbiology, Journal Year: 2025, Volume and Issue: 303, P. 110427 - 110427

Published: Feb. 11, 2025

Language: Английский

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ZCCHC3 inhibits PEDV proliferation by degrading nuclear coat proteins via the proteasome pathway

Veterinary Microbiology, Journal Year: 2025, Volume and Issue: 304, P. 110449 - 110449

Published: March 6, 2025

Language: Английский

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Current Evolutionary Dynamics of Porcine Epidemic Diarrhea Virus (PEDV) in the U.S. a Decade After Introduction

Viruses, Journal Year: 2025, Volume and Issue: 17(5), P. 654 - 654

Published: April 30, 2025

Porcine Epidemic Diarrhea Virus (PEDV) was introduced in the United States (U.S.) 2013, spreading rapidly and leading to economic losses. Two strains, S-INDEL non-S-INDEL, are present U.S. We analyzed 313 genomes 556 Spike protein sequences generated since its introduction. PEDV case numbers were highest during first two years after introduction (epidemic phase), then declined stabilized following (endemic phase). Sequence surveillance higher initial epidemic phase. Our results suggest non-S-INDEL strain is predominant The exhibit pairwise nucleotide identity percentages above 97.6%. Most sampled 2017 clustered into sub-clades. No descendants derived from other clades period detected contemporary data, suggesting that these no longer circulating currently restricted respective geographic regions our limited inter-regional spread. This insight helps determine risk of re-introduction if it regionally eliminated. Ongoing molecular essential confirming some older circulate anymore U.S., mapping distribution spread recent clades, understanding PEDV’s evolutionary diversification.

Language: Английский

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FoxO4 reduces the damage and mechanism of PEDV-infected IPEC-J2 cells through the NF-κB/MLCK pathway

Veterinary Microbiology, Journal Year: 2025, Volume and Issue: 306, P. 110568 - 110568

Published: May 19, 2025

Language: Английский

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Genetic and Pathogenic Analysis of a Novel Porcine Epidemic Diarrhea Virus Strain Isolated in the Republic of Korea

Viruses, Journal Year: 2024, Volume and Issue: 16(7), P. 1108 - 1108

Published: July 10, 2024

Porcine epidemic diarrhea (PED), caused by the porcine virus (PEDV), emerges annually in several Asian countries. Its major symptoms include watery diarrhea, vomiting, anorexia, and dehydration. PED outbreaks incur significant economic losses. The efficacy of vaccines is limited viral mutations insufficient intestinal mucosal immunity. Therefore, new against these recent variants are urgently needed. Herein, we isolated genetically characterized a novel Korean PEDV strain using NGS. Comparative genomic analysis demonstrated that CKK1-1 belonged to genogroup 2. was cultured sodium-glycochenodeoxycholic acid for 180 passages. Typically, isolation passage require proteases, such as trypsin. However, adapted this atypical culture condition, achieving high titer 8.83 ± 0.14 log TCID

Language: Английский

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Near zero background noise photoelectrochemical sensor based on sensitized signal probe CdS QDs-Dox intercalating double-stranded DNA for PEDV detection

Talanta, Journal Year: 2024, Volume and Issue: 281, P. 126826 - 126826

Published: Sept. 7, 2024

Language: Английский

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