The Clinical Impression of NDM-producing Acinetobacter baumannii in Intensive Care Units of the University Referral Hospital in North India DOI Open Access
Sangeeta Singh,

Anuragani Verma,

Vimala Venkatesh

et al.

Indian Journal of Critical Care Medicine, Journal Year: 2024, Volume and Issue: 28(11), P. 1044 - 1049

Published: Oct. 29, 2024

Carbapenem-resistant Acinetobacter baumannii (CRAb), a major public health threat, causes severe infections in Intensive Care Unit (ICU) patients. It resists β-lactam antibiotics through mechanisms like New Delhi metallo-beta-lactamase (NDM). In ICU patients, 69 species were isolated from 86 non-fermenting Gram-negative bacilli. Isolates identified using biochemical methods and Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS), carbapenem resistance detection was done by both phenotypic (mCIM eCIM) molecular methods. Out 66 A. baumannii, 61 carbapenem-resistant, with 20 confirmed as NDM producers. NDM-positive isolates exhibited higher associated significant mortality (75%). are pathogens poor outcomes. Key risk factors include prolonged stays, prior antimicrobial use, inadequate therapy. Early infection control crucial. patients linked to outcomes, highlighting the need for early measures. Singh S, Verma A, Venkatesh V, Reddy DH, Agrawal The Clinical Impression NDM-producing Units University Referral Hospital North India. Indian J Crit Med 2024;28(11):1044-1049.

Language: Английский

Clonality and the Phenotype–Genotype Correlation of Antimicrobial Resistance in Acinetobacter baumannii Isolates: A Multicenter Study of Clinical Isolates from Romania DOI Creative Commons
A Panà, Pavel Șchiopu, Dan Alexandru Țoc

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(1), P. 176 - 176

Published: Jan. 16, 2025

Antibiotic resistance is on the WHO's top 10 list of global public health threats due to its rapid emergence and spread but also because high morbidity mortality associated with it. Amongst main species driving this phenomenon A. baumannii, a member ESKAPE group medical assistance-associated infections causing famous for extensively drug-resistant phenotypes. Our findings note 91.52% frequency carbapenem-resistant baumannii (XDR CRAB) phenotype amongst clinical isolates from multiple hospitals in two major cities northwestern central Romania, harboring antibiotic genes such as blaOXA-23-like 108 (91.5%) isolates, blaOXA-24/40-like 88 (74.6%) blaNDM 29 (25%) ArmA 75 (63.6%) ant(3″)-I 69 (58.5%) sul1 113 (95.76%) isolates. The although nearly identical phenotype, displayed different genotypical profiles, varying degrees similarity across cities, raising possibility both local outbreaks single clone widespread dissemination resistant

Language: Английский

Citations

0
Integrated multi-omics reveals metabolic determinants of CRAB ST2 airway infection progression DOI Creative Commons
Ding-Yun Feng,

Jian-Xia Zhou,

Logen Liu

et al.

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

ABSTRACT Acinetobacter baumannii commonly causes lower airway colonization and infection is easily confused. This study aimed to analyze the biological characteristics of carbapenem-resistant A. (CRAB) ST2 in identify an effective method for distinguishing between infection. Lower CRAB isolated at Department Respiratory Critical Care Medicine intensive care unit our hospital from January 2021 June 2023 were included, their whole genome, biofilm-forming ability, bacterial virulence, metabolome analyzed. Fifty-six strains with identified, which 32 24 strains. The virulence resistance genes, as well ST2-type infecting colonizing similar. levels metabolites significantly airway-infecting than those airway-colonizing (S)-(+)−2-(aniline methyl) pyrrolidine, valine, ketoleucine, L-isoleucine, homoserine, N -acetyl-L-aspartate, 2-aminoethanol-1-phosphate Bacterial tests biofilm formation ability could not distinguish same ST or strains; however, metabolomics could. biosynthesis degradation pathways leucine, isoleucine downregulated, changes metabolism may be important factors promoting baumanniiCRAB transformation IMPORTANCE Carbapenem-resistant poses a critical threat clinical settings, particularly due challenges active infection, complicates treatment decisions. highlights limitations conventional approaches—such gene profiling, phenotypic assays, analysis—in differentiating infections versus colonization. By integrating metabolomics, we identified distinct metabolic signatures linked including significant downregulation biosynthesis/degradation reduced key (e.g., ketoleucine L-isoleucine) These findings provide first evidence that dysregulation drive CRAB’s transition invasive disease. work advances understanding pathogenicity offers novel, metabolism-based strategy improve diagnostic accuracy, guide targeted therapies, optimize antimicrobial stewardship managing CRAB-associated respiratory infections.

Language: Английский

Citations

0
Investigating the Effectiveness of Ceragenins against Acinetobacter baumannii to Develop New Antimicrobial and Anti-Adhesive Strategies DOI Open Access
Maciej Karasiński, Urszula Wnorowska, Tamara Daniluk

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(13), P. 7036 - 7036

Published: June 27, 2024

A growing body of experimental data indicates that ceragenins (CSAs), which mimic the physicochemical properties host's cationic antimicrobial peptide, hold promise for development a new group broad-spectrum antimicrobials. Here, using set in vivo experiments, we assessed potential eradication an important etiological agent nosocomial infections, Acinetobacter baumannii. Assessment bactericidal effect CSA-13, CSA-44, and CSA-131 on clinical isolates A. baumannii (n = 65) their effectiveness against bacterial cells embedded biofilm matrix after growth abiotic surfaces showed strong tested molecules regardless pattern. AFM assessment cell topography, stiffness, adhesion significant membrane breakdown rheological changes, indicating ability to target surface structures cells. In culture A549 lung epithelial cells, ceragenin CSA-13 had inhibit host suggesting it interferes with mechanism invasion. These findings highlight as therapeutic agents strategies infections caused by

Language: Английский

Citations

1
Identification of novel drug targets to counteract efflux pump mediated multidrug resistance in Acinetobacter baumannii DOI
Shyamalima Saikia, Indrani Gogoi, Minakshi Puzari

et al.

Gene Reports, Journal Year: 2024, Volume and Issue: 37, P. 102013 - 102013

Published: Aug. 16, 2024

Language: Английский

Citations

1
Prevalence, Characterization, and Epidemiological Relationships between ESBL and Carbapenemase-Producing Escherichia coli, Klebsiella pneumoniae, and Acinetobacter spp. Isolated from Humans and the Kitchen Environment of Two Greek Hospitals DOI Creative Commons
Anestis Tsitsos,

Alexandros Damianos,

Maria Boutel

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 13(10), P. 934 - 934

Published: Oct. 2, 2024

Background: Extended-spectrum-β-lactamase (ESBL) and carbapenemase-producing Enterobacterales Acinetobacter spp. pose significant challenges as nosocomial pathogens, demonstrating resistance against various antimicrobials. Their presence in food suggests that hospital kitchens could serve antibiotic reservoirs leading to patients’ infection. Objectives: The aim of this study was assess the prevalence characteristics β-lactam-resistant strains Escherichia coli, Klebsiella pneumoniae, isolated from kitchen environment staff two Greek hospitals. Methods: Strains were recovered after selective isolation with β-lactams identified MALDI–TOF MS. Antimicrobial susceptibility common β-lactamase genes evaluated. Protein profiles examined analyze potential relationships strain those patients. E. coli further categorized into phylogenetic groups. Results: overall 4.5%, 1.5%, 15.0% for K. spp., respectively, whereas human skin 4.0%. Almost all multidrug-resistant. All ESBL producers belonged phylogroups A B1. pneumoniae seven carbapenemase-producers. protein profile analysis showed relatedness between chicken strains, well patient originated either same or different Conclusions: results suggest may act important pathogen hotspots contributing circulation resistant environment.

Language: Английский

Citations

1
Colistin, doxycycline and Labetalol-meropenem combination are the most active against XDR-Carbapenem-resistant Acinetobacter baumannii: role of a novel transferrable plasmid conferring carbapenem resistance DOI
Heba Mohammed Refat M. Selim, Fatma Alzahraa M. Gomaa, Mohammad Y. Alshahrani

et al.

Diagnostic Microbiology and Infectious Disease, Journal Year: 2024, Volume and Issue: 110(4), P. 116558 - 116558

Published: Oct. 12, 2024

Language: Английский

Citations

1
The Clinical Impression of NDM-producing Acinetobacter baumannii in Intensive Care Units of the University Referral Hospital in North India DOI Open Access
Sangeeta Singh,

Anuragani Verma,

Vimala Venkatesh

et al.

Indian Journal of Critical Care Medicine, Journal Year: 2024, Volume and Issue: 28(11), P. 1044 - 1049

Published: Oct. 29, 2024

Carbapenem-resistant Acinetobacter baumannii (CRAb), a major public health threat, causes severe infections in Intensive Care Unit (ICU) patients. It resists β-lactam antibiotics through mechanisms like New Delhi metallo-beta-lactamase (NDM). In ICU patients, 69 species were isolated from 86 non-fermenting Gram-negative bacilli. Isolates identified using biochemical methods and Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS), carbapenem resistance detection was done by both phenotypic (mCIM eCIM) molecular methods. Out 66 A. baumannii, 61 carbapenem-resistant, with 20 confirmed as NDM producers. NDM-positive isolates exhibited higher associated significant mortality (75%). are pathogens poor outcomes. Key risk factors include prolonged stays, prior antimicrobial use, inadequate therapy. Early infection control crucial. patients linked to outcomes, highlighting the need for early measures. Singh S, Verma A, Venkatesh V, Reddy DH, Agrawal The Clinical Impression NDM-producing Units University Referral Hospital North India. Indian J Crit Med 2024;28(11):1044-1049.

Language: Английский

Citations

1