Progresses and Prospects on Glucosinolate Detection in Cruciferous Plants DOI Creative Commons
X.L. Li,

Dongna Wen,

Yafei He

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(24), P. 4141 - 4141

Published: Dec. 20, 2024

This review provides a comprehensive summary of the latest international research on detection methods for glucosinolates in cruciferous plants. article examines various analytical techniques, including high-performance liquid chromatography (HPLC), chromatography–mass spectrometry (LC-MS), enzyme-linked immunosorbent assay (ELISA), and capillary electrophoresis (CE), while highlighting their respective advantages limitations. Additionally, this delves into recent advancements sample preparation, extraction, quantification methods, offering valuable insights accurate efficient determination glucosinolate content across diverse plant materials. Furthermore, it underscores critical importance standardization validation these methodologies to ensure reliable analyses both scientific industrial applications.

Language: Английский

Application and development of CRISPR technology in the secondary metabolic pathway of the active ingredients of phytopharmaceuticals DOI Creative Commons

Haixin Gao,

Xinyi Pei,

Xinyu Song

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 9, 2025

As an efficient gene editing tool, the CRISPR/Cas9 system has been widely employed to investigate and regulate biosynthetic pathways of active ingredients in medicinal plants. CRISPR technology holds significant potential for enhancing both yield quality By precisely regulating expression key enzymes transcription factors, not only deepens our understanding secondary metabolic plants but also opens new avenues drug development modernization traditional Chinese medicine. This article introduces principles its efficacy editing, followed by a detailed discussion applications metabolism includes examination composition implementation strategies within pathways, as well influence Cas9 protein variants advanced systems field. In addition, this examines long-term impact on progress plant research development. It raises existing issues research, including off-target effects, complexity genome structure, low transformation efficiency, insufficient pathways. At same time, puts forward some insights order provide ideas subsequent application summary, presents broad prospects study is poised facilitate further advancements biomedicine agricultural science. technological continue challenges are progressively addressed, expected play increasingly vital role

Language: Английский

Citations

1
Development and optimization of multiplex PCR for rapid detection of type I-F1 and type I-F2 Cas cluster genes in Acinetobacter baumannii DOI

Gulshan Yadav,

Amit Sharma,

Umesh Prasad Sah Hathi

et al.

Biologicals, Journal Year: 2025, Volume and Issue: 90, P. 101824 - 101824

Published: March 13, 2025

Language: Английский

Citations

0
Advances of Progress and Prospects on Glucosinolate Detection in Cruciferous Plants DOI Open Access

Xuan er Li,

Dong Wen,

Yu He

et al.

Published: Nov. 6, 2024

This review provides a comprehensive summary of the latest international research on detection methods for glucosinolates in cruciferous plants. The article examines various analytical tech-niques, including high-performance liquid chromatography (HPLC), chromatog-raphy-mass spectrometry (LC-MS), and enzyme-linked immunosorbent assay (ELISA), while highlighting their respective advantages limitations. Additionally, delves into recent advancements sample preparation, extraction, quantification methods, offering valuable insights accurate efficient determination glucosinolate content across diverse plant materials. Furthermore, it underscores critical importance standardization validation these methodologies to ensure reliable analysis both scientific industrial applications.

Language: Английский

Citations

1
Optimizing Brassica oleracea L. Breeding Through Somatic Hybridization Using Cytoplasmic Male Sterility (CMS) Lines: From Protoplast Isolation to Plantlet Regeneration DOI Creative Commons
Miriam Romero-Muñoz, Margarita Pérez‐Jiménez

Plants, Journal Year: 2024, Volume and Issue: 13(22), P. 3247 - 3247

Published: Nov. 19, 2024

The

Language: Английский

Citations

0
Progresses and Prospects on Glucosinolate Detection in Cruciferous Plants DOI Creative Commons
X.L. Li,

Dongna Wen,

Yafei He

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(24), P. 4141 - 4141

Published: Dec. 20, 2024

This review provides a comprehensive summary of the latest international research on detection methods for glucosinolates in cruciferous plants. article examines various analytical techniques, including high-performance liquid chromatography (HPLC), chromatography–mass spectrometry (LC-MS), enzyme-linked immunosorbent assay (ELISA), and capillary electrophoresis (CE), while highlighting their respective advantages limitations. Additionally, this delves into recent advancements sample preparation, extraction, quantification methods, offering valuable insights accurate efficient determination glucosinolate content across diverse plant materials. Furthermore, it underscores critical importance standardization validation these methodologies to ensure reliable analyses both scientific industrial applications.

Language: Английский

Citations

0