Characterization of the complete chloroplast genome and development of molecular markers of Salix DOI Creative Commons
Pu Wang, Jiahui Guo, Jie Zhou

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 18, 2024

Salix, an economically and ecologically multifunctional tree species widely distributed in China, encompasses five ornamental sequenced this study, which are highly beneficial for plant phytoremediation due to their ability absorb heavy metals. This research utilized high-throughput sequencing acquire chloroplast genome sequences of analyzing gene composition structural characteristics, identifying potential molecular markers, laying a foundation Salix identification resource classification. Chloroplast DNA was extracted from the leaves argyracea, dasyclados, eriocephala, integra 'Hakuro Nishiki', suchowensis using optimized CTAB method. Sequencing conducted on Illumina NovaSeq PE150 platform, bioinformatics tools were employed compare features variations within genomes Salix. Analysis revealed high similarity among species, with subsequent examination 276, 269, 270, 273, 273 SSR loci, respectively, along unique simple repeat each variety. Comparison across 22 highlighted regions such as matK-trnQ, ndhC-trnV, psbE-petL, rpl36-rps8, ndhB-rps7, may serve valuable markers willow classification studies. In analysis not only enhances genetic resources but also forms critical basis development exploration interspecific phylogeny genus.

Language: Английский

Decoding the Chloroplast Genome of Bitterwood (Picrasma quassioides): Structure, Variability, and Evolutionary Relationships DOI Creative Commons
Qin Liu,

Huaxi Huang,

Jinhang Lin

et al.

Ecology and Evolution, Journal Year: 2025, Volume and Issue: 15(4)

Published: April 1, 2025

The chloroplast genome of Picrasma quassioides, a medicinally significant plant in traditional Asian medicine, was sequenced and analyzed to understand its genetic architecture evolutionary relationships within Simaroubaceae. High-throughput sequencing revealed 160,013-bp circular with typical quadripartite structure, encoding 132 genes including 87 protein-coding genes, 37 tRNA 8 rRNA genes. Comparative analysis other Simaroubaceae species identified distinct patterns nucleotide diversity between single-copy repeat regions, while examination IR boundaries dynamic processes. Analysis 101 SSR loci 48 sequences provided insights into organization potential molecular markers for identification. Selective pressure across 78 demonstrated predominant purifying selection (average K a/K s ratio 0.23), evidence positive specific Phylogenetic reconstruction using 77 confirmed Simaroubaceae's monophyly close relationship Rutaceae. These findings advance our understanding evolution providing tools authentication breeding this valuable medicinal species. comprehensive genomic characterization establishes foundation investigating the basis therapeutic properties P. quassioides facilitating conservation.

Language: Английский

Citations

0
Characterization of the complete chloroplast genome and development of molecular markers of Salix DOI Creative Commons
Pu Wang, Jiahui Guo, Jie Zhou

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 18, 2024

Salix, an economically and ecologically multifunctional tree species widely distributed in China, encompasses five ornamental sequenced this study, which are highly beneficial for plant phytoremediation due to their ability absorb heavy metals. This research utilized high-throughput sequencing acquire chloroplast genome sequences of analyzing gene composition structural characteristics, identifying potential molecular markers, laying a foundation Salix identification resource classification. Chloroplast DNA was extracted from the leaves argyracea, dasyclados, eriocephala, integra 'Hakuro Nishiki', suchowensis using optimized CTAB method. Sequencing conducted on Illumina NovaSeq PE150 platform, bioinformatics tools were employed compare features variations within genomes Salix. Analysis revealed high similarity among species, with subsequent examination 276, 269, 270, 273, 273 SSR loci, respectively, along unique simple repeat each variety. Comparison across 22 highlighted regions such as matK-trnQ, ndhC-trnV, psbE-petL, rpl36-rps8, ndhB-rps7, may serve valuable markers willow classification studies. In analysis not only enhances genetic resources but also forms critical basis development exploration interspecific phylogeny genus.

Language: Английский

Citations

0