Differential Gene and Protein Expressions Responsible for Vasomotor Signaling Provide Mechanistic Bases for the Opposite Flow-Induced Responses of Pre- and Post-Circle of Willis Arteries DOI Creative Commons
Zoltán Németh, Krisztián Erős, Gyöngyi Munkácsy

et al.

Life, Journal Year: 2025, Volume and Issue: 15(6), P. 856 - 856

Published: May 26, 2025

Increases in flow elicit dilations the basilar artery (BA) supplied by posterior cerebral circulation (PCC), and ensuring efficient blood supply to circle of Willis which pressure can distribute equalize, thus provide appropriate for daughter branches reach certain brain areas. In contrast, increases constrictions middle (MCA), anterior (ACC) regulating distal circulation. Mediators flow-dependent responses include arachidonic acid (AA) metabolites nitric oxide (NO). We hypothesized that mediators are differentially expressed arteries PCC (CAPCC) ACC (CAACC). The expressions key enzymes AA pathway—cyclooxygenases (COX1/COX2), cytochrome P450 hydroxylases (Cyp450), thromboxane synthase (TXAS), A2 (TP) receptor, prostacyclin (PGIS), (IP) receptor (IP); neuronal (nNOS), endothelial (eNOS)—in BA MCA from rats (n = 20) were determined western blotting. Transcriptome analysis CAPCC CAACC 25) was assessed RNA sequencing. compared MCA, COX1/2 Cyp450 protein lower, PGIS higher, TXAS nNOS/eNOS similar, TP receptors IP higher. Gene vasodilator canonical pathways higher CAPCC; vasoconstriction CAACC. vasomotor signaling circulation, corresponding their function.

Language: Английский

Identifying KLF14 as a potential regulatory factor in liver regeneration trough transcriptomic and metabolomic DOI Creative Commons
Chang Liu, Dalong Zhu,

Junlong Xue

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 3, 2025

Liver regeneration is a complex process crucial for recovery after partial hepatectomy (PH) or ex-vivo liver resection and autotransplantation (ELRA). This study aimed to explore the molecular mechanisms involved in by analyzing peripheral blood samples from three patients with alveolar echinococcosis undergoing PH ELRA. Peripheral were collected ELRA at time points: pre-operation, postoperative day 1, 5, as well healthy controls. Transcriptomic analysis was performed identify differentially expressed genes (DEGs) using RNA sequencing, while metabolomic conducted untargeted liquid chromatography-mass spectrometry (LC-MS). Key findings validated through real-time quantitative polymerase chain reaction (RT-qPCR) Western blot analysis. revealed 3574 DEGs on post-operative 1 compared pre-operation group, 3269 5 1. In 1619 identified 896 found Among these, 36 common shared between both groups, primarily enriched metabolic pathways. Integration of genes, co-expression network Mfuzz clustering KLF14 gene correlated processes, its association PI3K-AKT pathway. Metabolomic highlighted metabolites associated lipid, amino acid, energy metabolism. provides new insights into regulation regeneration, identifying processes. These offer potential therapeutic targets enhancing repair.

Language: Английский

Citations

0
Integrative Analysis of Arachidonic Acid Metabolism in the Pathogenesis and Immune Dysregulation of Psoriasis DOI Creative Commons

Mengyi Hou,

Yanting Sun

Clinical Cosmetic and Investigational Dermatology, Journal Year: 2025, Volume and Issue: Volume 18, P. 601 - 615

Published: March 1, 2025

Psoriasis is a chronic inflammatory skin disorder with complex molecular mechanisms. While previous studies have demonstrated altered levels of arachidonic acid and its metabolites in psoriatic lesions, the specific roles metabolism (AAM) genes pathogenesis immune dysregulation psoriasis remain poorly understood. This study aimed to investigate role AAM using an integrative bioinformatics approach. Gene expression data from patients healthy controls were obtained Expression Omnibus database analyzed. Differentially expressed identified, functional enrichment analyses performed. Weighted gene co-expression network analysis (WGCNA) machine learning techniques employed identify associated genes. Single-sample set (ssGSEA) cell composition conducted explore implications. Transcription factor prediction was performed potential regulators key Differential revealed 469 dysregulated psoriasis, highlighting involvement epidermis development, response, inflammation. WGCNA approaches identified ABCC1, PLA2G3, CYP2J2, GPX2 as ssGSEA showed elevated inflammation response correlating pathways. Immune increased infiltration cells skin. shared transcription factors for genes, suggesting coordinated regulation their psoriasis. dysregulation, providing novel insights into basis The findings highlight therapeutic targets biomarkers, which could lead improved diagnosis treatment strategies this disorder.

Language: Английский

Citations

0
Comprehensive Analysis of Nigella sativa (L.) Pharmacology: A Multi-Method Approach with ICP, HPLC-DAD, in Vitro, in Vivo, and in Silico Techniques DOI
Otmane Zouirech,

Assia Houiat,

Rafik El-Mernissi

et al.

Journal of Molecular Structure, Journal Year: 2025, Volume and Issue: unknown, P. 142182 - 142182

Published: March 1, 2025

Language: Английский

Citations

0
Differential Gene and Protein Expressions Responsible for Vasomotor Signaling Provide Mechanistic Bases for the Opposite Flow-Induced Responses of Pre- and Post-Circle of Willis Arteries DOI Creative Commons
Zoltán Németh, Krisztián Erős, Gyöngyi Munkácsy

et al.

Life, Journal Year: 2025, Volume and Issue: 15(6), P. 856 - 856

Published: May 26, 2025

Increases in flow elicit dilations the basilar artery (BA) supplied by posterior cerebral circulation (PCC), and ensuring efficient blood supply to circle of Willis which pressure can distribute equalize, thus provide appropriate for daughter branches reach certain brain areas. In contrast, increases constrictions middle (MCA), anterior (ACC) regulating distal circulation. Mediators flow-dependent responses include arachidonic acid (AA) metabolites nitric oxide (NO). We hypothesized that mediators are differentially expressed arteries PCC (CAPCC) ACC (CAACC). The expressions key enzymes AA pathway—cyclooxygenases (COX1/COX2), cytochrome P450 hydroxylases (Cyp450), thromboxane synthase (TXAS), A2 (TP) receptor, prostacyclin (PGIS), (IP) receptor (IP); neuronal (nNOS), endothelial (eNOS)—in BA MCA from rats (n = 20) were determined western blotting. Transcriptome analysis CAPCC CAACC 25) was assessed RNA sequencing. compared MCA, COX1/2 Cyp450 protein lower, PGIS higher, TXAS nNOS/eNOS similar, TP receptors IP higher. Gene vasodilator canonical pathways higher CAPCC; vasoconstriction CAACC. vasomotor signaling circulation, corresponding their function.

Language: Английский

Citations

0