Integrated RNA-Seq and Metabolomics Analyses of Biological Processes and Metabolic Pathways Involved in Seed Development in Arachis hypogaea L.

Genes, Journal Year: 2025, Volume and Issue: 16(3), P. 300 - 300

Published: Feb. 28, 2025

In peanut cultivation, fertility and seed development are essential for fruit quality yield, while pod number per plant, pod, kernel weight, size indicators of yield. this study, metabolomic RNA-seq analyses were conducted on the flowers aerial pegs (aerpegs) two cultivars JNH3 (Jinonghei) SLH (Silihong), respectively. Compared with SLH, had 3840 up-regulated flower-specific differentially expressed genes (DEGs) 5890 aerpeg-specific DEGs. aerpegs, there 4079 variety-specific DEGs 18 accumulated metabolites (DAMs) flowers, 3732 48 DAMs in flowers. Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) enrichment revealed that associated pollen germination phenylalanine metabolism flower aerpeg tissues, contrast, protein degradation, amino acid metabolism, DNA repair. However, significant differences lipids lipid-like molecules between This investigation provides candidate an experimental basis further improvement high-quality high-yield varieties.

Language: Английский

Fine mapping and candidate gene analysis of major QTLs for number of seeds per pod in Arachis hypogaea L.

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: April 15, 2025

Peanut (Arachis hypogaea L., 2n = 2x 20) is an important industrial and oil crop that widely grown in more than 100 countries. In recent years, breeders have focused on increasing the seed number per pod to improve their yield addition other breeding for key components of yield, including number, seeds pod, 100-seed weight. this study, a secondary population 1,114 BC1F2 lines was derived from cross between parents R45 JNH3. Two stable major-effect quantitative trait loci qRMPA09.1 qRMPA09.2 were detected simultaneously mapped within chromosomal intervals approximately 400 Kb 600 chromosome A09. Additionally, combined whole-genome RNA sequencing analyses showed differential expression Arahy.04JNDX gene belongs MYB transcription factor (TF) two parents. The AhMYB51 also inferred influence peanuts. An examination backcross L2/L4 increases rate multiple pods plant (RMSP) primarily by affecting brassinosteroids flowers, while its overexpression promotes length siliques Arabidopsis thaliana. Our findings provide valuable insights cloning favorable alleles RMSP qRMSPA09.1 qRMSPA09.2 are novel QTL associated with trait, predicted as candidate gene. Moreover, closely linked polymorphic SNP markers significant QTLs may be useful accelerating marker-assisted

Language: Английский