Influence of Zinc Oxide Nanoparticles in In Vitro Culture and Bacteria Bacillus thuringiensis in Ex Vitro Conditions on the Growth and Development of Blackberry (Rubus fruticosus L.)

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(9), P. 3743 - 3743

Published: April 27, 2024

The blackberry, valued for its delicious fruit, has gained attention medicinal bioactive compounds. In vitro cultivation methods, including nanoparticle enhancement, are increasingly chosen due to their advantages over traditional propagation techniques. We tested the effect of commercial zinc oxide nanoparticles (ZnONPs) on growth and development blackberry (Rubus fruticosus L.) Navaho variety in an culture MS medium supplemented with 0.6 mg dm−3 BA, 0.1 IBA, 0.01 GA3, various concentrations nanoparticles: 0 (control), 10, 20, 30, 40 dm−3. morphological features plantlets were assessed two three months after start culture. Selected biological characteristics determined. values parameters from depended concentration ZnONPs medium. Increasing negatively affected number length shoots roots fresh weight plantlets. total phenolic content treatments was lower than control plants, but antioxidant capacity as measured by ABTS method higher. chlorophyll a, b, chlorophyll, carotenoids decreased at higher addition increased reduced iron Concentrations 10–30 potassium, calcium, magnesium, zinc, manganese, copper, while highest ZnONPs, these minerals similar control, except a calcium manganese. growing presence acclimatized ex conditions soil inoculated Bacillus thuringiensis. Bacteria added substrate favourably influenced plants weight.

Language: Английский

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Biotransformation of organic waste into compost: Implications for in vitro conservation of Withania coagulans (Stocks) Dunal

In Vitro Cellular & Developmental Biology - Plant, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

Language: Английский

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A bibliometric analysis of research on blackberry micropropagation

Plant Cell Tissue and Organ Culture (PCTOC), Journal Year: 2025, Volume and Issue: 160(2)

Published: Jan. 29, 2025

Language: Английский

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Agar concentration interferes with the biometry, photosynthetic pigment content, and anatomy of Selenicereus undatus in vitro

Ciência e Agrotecnologia, Journal Year: 2025, Volume and Issue: 49

Published: Jan. 1, 2025

ABSTRACT The micropropagation of dragon fruit (Selenicereus undatus) is an alternative method to produce vigorous plants with high phytosanitary quality. However, depending on the consistency growing medium (liquid, semi-solid, and solid), can develop physiological anatomical disorders, impairing their growth reducing viability ex vitro. aim this study was evaluate biometric characteristics, photosynthetic pigments, sections S. undatus grown in five concentrations agar (0.0, 3.5, 7.0, 10.5, 14.0 g L-1) MS medium. Biometric characteristics (number roots, length aerial part, fresh dry masses roots part plants), pigments (chlorophyll a, b, total carotenoid contents), anatomy [number vascular bundles, cross-sectional area (mm2), reserve parenchyma (μm)] were evaluated. Biometric, pigment, differed between for different agar. Plants agar-free showed increased length, root biomass, indicating enhanced growth. In contrast, media containing 10.5 L-¹ exhibited higher levels chlorophyll chlorophyll. Additionally, agar, regardless concentration. without bundle count, area, than those way, be micropropagated efficiently

Language: Английский

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Cardiometabolic Risk: Characteristics of the Intestinal Microbiome and the Role of Polyphenols

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(18), P. 13757 - 13757

Published: Sept. 6, 2023

Cardiometabolic diseases like hypertension, type 2 diabetes mellitus, atherosclerosis, and obesity have been associated with changes in the gut microbiota structure, or dysbiosis. The beneficial effect of polyphenols on reducing incidence this chronic disease has confirmed by numerous studies. Polyphenols are primarily known for their anti-inflammatory antioxidant properties, but they can also modify microbiota. According to recent research, positively influence microbiota, which regulates metabolic responses reduces systemic inflammation. This review emphasizes prebiotic role impact specific components patients at cardiometabolic risk. It analyzes most research positive effects health. While vitro vivo studies shown interaction involving additional clinical investigations required assess people.

Language: Английский

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Methods of Blackberry Propagation in vitro Condition

BIO Web of Conferences, Journal Year: 2024, Volume and Issue: 85, P. 01009 - 01009

Published: Jan. 1, 2024

Blackberry, one of the most important fruit species belonging to Rosaceae family, can be grown in different environments due having wide adaptation ability. Although it originated Europe, today common cultivars have North American origin. Also, expansion its production last 25 years especially Europe and USA, blackberry has become fourth berry fresh market after strawberries, blueberries, raspberries. Blackberry fruits are rich vitamins, polyphenols, minerals, antioxidants, gallic acid routine. Many studies proven that high nutritional composition a positive effect on human health preventing various diseases. It an place processed market. Frozen used such as ice cream, juice, jam, marmalade, cake, sweet products. breeding been ongoing for over 100 increase yield quality, thornless cane, improve disease insect resistance, cane management primocane fruiting. In vitro propagation is alternative method introduce new quickly into provide disease-free planting material compared with traditional methods. The purpose this study was summarize methods conditions.

Language: Английский

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Microbiological Biostimulants in the Improvement of Extended Storage Quality of In Vitro-Derived Plants of Popular Ornamental Perennials

Agronomy, Journal Year: 2024, Volume and Issue: 14(2), P. 289 - 289

Published: Jan. 27, 2024

In vitro propagation is a crucial method for the mass production of high-quality plants, but impact microbiological interventions during ex storage remains an underexplored aspect. This study aims to assess effects three commercial biostimulants in form preparations—BactoFungiStop, AzotoPower, and Guard—applied over six months through foliar sprays on post-storage quality Brunnera macrophylla ‘Silver Heart’, Echinacea purpurea ‘Secret Glow’, Heuchera × hybrida ‘Northern Exposure Red’, Persicaria amplecicaulis ‘JS Caliente’, Rudbeckia ‘Sunbeckia Sophia Yellow’ plants. The monthly application preparations adhered concentrations recommended by producers. Post-storage evaluations included shoot root parameters, leaf morphology, chlorophyll biosynthesis. All positively influenced elongation B. Heart’. treatments stimulated development this species, i.e., increased length, area, volume, number forks tips. E. all enhanced traits, with Guard demonstrating highest efficacy. As P. BactoFungiStop negatively affected parameters promoted development. Red’ exhibited dimensions treatments, while displayed positive responses shoot-related traits no None biosynthesis any studied species. results our research can be implemented large-scale ornamental

Language: Английский

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Molecular Marker Applications in the Selection of Elite Genotypes for Plant Stress Tolerance and Genetic Fidelity

OBM Genetics, Journal Year: 2024, Volume and Issue: 08(03), P. 1 - 25

Published: Sept. 2, 2024

Molecular markers play a crucial role in accelerating crop production for sustainable agriculture by identifying resistant traits and enhancing genetic diversity. In this review, we examine the impact of molecular on advancing our understanding stress tolerance mechanisms plants, addressing pressing imperative to bolster global food meet escalating demands growing population. Additionally, application evaluate accuracy plants due changes caused somaclonal variation during <em>in vitro</em> propagation is mentioned. Specifically, highlight emerging technologies like MAS, MARS, MABC, GWAS, GS, DH production, speed breeding, genome editing, which offer promising opportunities enhance integrity cultivars, aiding security challenges. The literature search focused studies published last ten years. We utilized combination specific broad keywords such as stability, propagation, markers, abiotic-biotic stress, plant biotechnology. conclusion, review analyzes use assessing fidelity grown plants.

Language: Английский

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Genetic Diversity and Structure of Quercus petraea (Matt.) Liebl. Populations in Central and Northern Romania Revealed by SRAP Markers

Diversity, Journal Year: 2023, Volume and Issue: 15(10), P. 1093 - 1093

Published: Oct. 19, 2023

The genetic variability of five populations Quercus petraea originating from the Transylvania and Maramureș regions Romania was investigated in this study to provide insights into species’ adaptability, population dynamics, potential for preservation face environmental challenges. To achieve this, sequence-related amplified polymorphism (SRAP) markers, conjunction with a set 18 primer combinations, were employed. outcomes analysis revealed range polymorphisms spanning 69.78% 85.75%. Additionally, assessment diversity using Shannon’s information index (I) yielded values ranging between 0.2887 0.3955, while Nei’s gene (He) exhibited spectrum 0.1833 0.2582. variability, conducted via molecular variance (AMOVA), unveiled that 9% variation attributable differences among populations, substantial 91% resided within populations. A further investigation structure construction UPGMA dendrogram based on distances elucidated presence two principal clusters, finding reinforced by Principal Coordinate Analysis (PCoA). SRAP markers offers promising upcoming breeding programs identify optimal genitors, facilitating development well-adapted oak regions.

Language: Английский

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