Extensive comparison of salivary collection, transportation, preparation, and storage methods: a systematic review DOI Creative Commons
Hamed Mortazavi, Amir‐Ali Yousefi‐Koma, Hannaneh Yousefi‐Koma

et al.

BMC Oral Health, Journal Year: 2024, Volume and Issue: 24(1)

Published: Feb. 2, 2024

Abstract Background Human saliva as a bodily fluid—similar to blood—is utilized for diagnostic purposes. Unlike blood sampling, collecting is non-invasive, inexpensive, and readily accessible. There are no previously published systematic reviews regarding different collection, transportation, preparation, storage methods human saliva. Design This study has been prepared organized according the preferred reporting items meta-analyses (PRISMA) 2020 guidelines. review registered at PROSPERO (Registration ID: CRD42023415384). The question PICO format was followed: Comparison of performance (C) handling, techniques (I) assessed analyzing stimulated or unstimulated (P O). An electronic search executed in Scopus, Google Scholar, PubMed. Results Twenty-three descriptive clinical studies between 1995 2022 were included. Eight categories salivary features biomarkers investigated (i.e., flow rate, total quantity, protein, cortisol, testosterone, DNA quality pH buffering pH). Twenty-two sampling methods/devices utilized. Passive drooling, Salivette®, spitting most methods. Sampling times with optimum capabilities iodine, oral cancer metabolites suggested be 7:30 AM 9:00 AM, 10:30 11:00 14:00 PM 20:00 PM, respectively. 6 Centrifuging samples storing them -70 °C -80 method. For quantity quality, immediately after collection without centrifuging storage, outperformed °C. Non-coated Salivette® successful method/device rate. Conclusion It highly that scientists take aid from reported categorized outcomes, design their questions based on current voids each method/device.

Language: Английский

Extensive comparison of salivary collection, transportation, preparation, and storage methods: a systematic review DOI Creative Commons
Hamed Mortazavi, Amir‐Ali Yousefi‐Koma, Hannaneh Yousefi‐Koma

et al.

BMC Oral Health, Journal Year: 2024, Volume and Issue: 24(1)

Published: Feb. 2, 2024

Abstract Background Human saliva as a bodily fluid—similar to blood—is utilized for diagnostic purposes. Unlike blood sampling, collecting is non-invasive, inexpensive, and readily accessible. There are no previously published systematic reviews regarding different collection, transportation, preparation, storage methods human saliva. Design This study has been prepared organized according the preferred reporting items meta-analyses (PRISMA) 2020 guidelines. review registered at PROSPERO (Registration ID: CRD42023415384). The question PICO format was followed: Comparison of performance (C) handling, techniques (I) assessed analyzing stimulated or unstimulated (P O). An electronic search executed in Scopus, Google Scholar, PubMed. Results Twenty-three descriptive clinical studies between 1995 2022 were included. Eight categories salivary features biomarkers investigated (i.e., flow rate, total quantity, protein, cortisol, testosterone, DNA quality pH buffering pH). Twenty-two sampling methods/devices utilized. Passive drooling, Salivette®, spitting most methods. Sampling times with optimum capabilities iodine, oral cancer metabolites suggested be 7:30 AM 9:00 AM, 10:30 11:00 14:00 PM 20:00 PM, respectively. 6 Centrifuging samples storing them -70 °C -80 method. For quantity quality, immediately after collection without centrifuging storage, outperformed °C. Non-coated Salivette® successful method/device rate. Conclusion It highly that scientists take aid from reported categorized outcomes, design their questions based on current voids each method/device.

Language: Английский

Citations

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