Monitoring the Emergence of SARS-CoV-2 VOCs in Wastewater and Clinical Samples—A One-Year Study in Santiago de Compostela (Spain) DOI Creative Commons
Marta Lois, David Polo, María de Toro

et al.

Viruses, Journal Year: 2025, Volume and Issue: 17(4), P. 489 - 489

Published: March 28, 2025

Wastewater surveillance has become a valuable tool to monitor the emergence of SARS-CoV-2 variants concern (VOCs) at community level. In this study, we aimed evaluate presence Alpha (B.1.1.7), Beta (B.1.351), Delta (B.1617.2), and Omicron (B.1.1.529) VOCs in samples from inlet wastewater treatment plant (WWTP) as well two different sewer interceptors (SI-1 SI-2) urban sewage system Santiago de Compostela (Galicia, NW Spain) throughout 2021 January 2022. For purpose, detection quantification four was performed using duplex allelic discrimination RT-qPCR assays, targeting S-gene. An N1 gene assay used reference for RNA samples. All were detected Alpha, Beta, Delta, 45.7%, 7.5%, 66.7%, 72.7% all samples, respectively. VOC dominant during first part whereas peaks observed May–June December 2021, Some differences among results obtained city sectors studied, which could be explained by characteristics population between them. Wastewater-based epidemiology allowed us track early circulation local level, our are temporally concordant with clinical data epidemiological findings reported health authorities.

Language: Английский

Monitoring the Emergence of SARS-CoV-2 VOCs in Wastewater and Clinical Samples—A One-Year Study in Santiago de Compostela (Spain) DOI Creative Commons
Marta Lois, David Polo, María de Toro

et al.

Viruses, Journal Year: 2025, Volume and Issue: 17(4), P. 489 - 489

Published: March 28, 2025

Wastewater surveillance has become a valuable tool to monitor the emergence of SARS-CoV-2 variants concern (VOCs) at community level. In this study, we aimed evaluate presence Alpha (B.1.1.7), Beta (B.1.351), Delta (B.1617.2), and Omicron (B.1.1.529) VOCs in samples from inlet wastewater treatment plant (WWTP) as well two different sewer interceptors (SI-1 SI-2) urban sewage system Santiago de Compostela (Galicia, NW Spain) throughout 2021 January 2022. For purpose, detection quantification four was performed using duplex allelic discrimination RT-qPCR assays, targeting S-gene. An N1 gene assay used reference for RNA samples. All were detected Alpha, Beta, Delta, 45.7%, 7.5%, 66.7%, 72.7% all samples, respectively. VOC dominant during first part whereas peaks observed May–June December 2021, Some differences among results obtained city sectors studied, which could be explained by characteristics population between them. Wastewater-based epidemiology allowed us track early circulation local level, our are temporally concordant with clinical data epidemiological findings reported health authorities.

Language: Английский

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