Rosmarinic acid improves boar sperm quality, antioxidant capacity and energy metabolism at 17°C via AMPK activation DOI
Tianyu Feng,

Dong‐Liang Lv,

Xing Zhang

et al.

Reproduction in Domestic Animals, Journal Year: 2020, Volume and Issue: 55(12), P. 1714 - 1724

Published: Sept. 24, 2020

Boar sperm are susceptible to oxidative damage caused by reactive oxygen species (ROS) during storage. Adenosine monophosphate (AMP)-activated protein kinase (AMPK) is an important therapeutic target, because it a cellular metabolism energy sensor and key signalling in spermatozoa. We evaluated the effects of rosmarinic acid (RA), antioxidant, on boar liquid storage determine whether protects via AMPK activation. ejaculates were diluted with Modena extender different concentrations RA stored at 17°C for 9 days. Sperm quality parameters, antioxidant capacity, metabolism, phosphorylation fertility analysed. Compared control, 40 μmol/L significantly improved motility, plasma membrane integrity acrosome (p < .05). The effective time was up On third seventh days, exhibited increased total capacity (T-AOC), superoxide dismutase (SOD) activity, adenosine triphosphate (ATP) content, mitochondrial potential (ΔΨm) glyceraldehyde-3-phosphate dehydrogenase (GAPDH) whereas malondialdehyde (MDA) content decreased Western blot showed that RA, as well AICAR (AMPK activator), promoted phosphorylation, Compound C inhibitor) inhibited this effect. sperm-zona pellucida binding experiment number attached zona These findings suggest meaningful methods preservation vitro provide new insights into mechanism which cells from

Language: Английский

Roles of AMP-Activated Protein Kinase (AMPK) in Mammalian Reproduction DOI Creative Commons
Weina Yang, Lingjuan Wang, Fengli Wang

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2020, Volume and Issue: 8

Published: Nov. 19, 2020

Reproduction is an energy demanding function and only take place in case of sufficient available status mammals. Metabolic diseases such as anorexia nervosa are clinically associated with reduced fertility. AMP-activated protein kinase (AMPK), a major regulator cellular homeostasis, activated limited reserves to ensure the orderly progress various physiological activities. In recent years, mounting evidence shows that AMPK involved regulation reproductive through multiple mechanisms. likely be metabolic sensor integrating central peripheral signals. this review, we aim explore preclinical studies published last decade investigate role field, its target for drug therapy system-related diseases. We also emphasized emerging roles transcriptional reproduction processes metabolisms, which tightly related state fertility organism.

Language: Английский

Citations

38
Energy sensors and reproductive hypothalamo-pituitary ovarian axis (HPO) in female mammals: Role of mTOR (mammalian target of rapamycin), AMPK (AMP-activated protein kinase) and SIRT1 (Sirtuin 1) DOI Creative Commons
Anthony Estienne, Alice Bongrani,

Christelle Ramé

et al.

Molecular and Cellular Endocrinology, Journal Year: 2020, Volume and Issue: 521, P. 111113 - 111113

Published: Dec. 7, 2020

Language: Английский

Citations

33
Bacterial Infections Affect Male Fertility: A Focus on the Oxidative Stress-Autophagy Axis DOI Creative Commons
Sutian Wang, Kunli Zhang,

Yuchang Yao

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2021, Volume and Issue: 9

Published: Oct. 21, 2021

Numerous factors trigger male infertility, including lifestyle, the environment, health, medical resources and pathogenic microorganism infections. Bacterial infections of reproductive system can cause various diseases. Several organs, such as testicles, have unique immune functions that protect germ cells from damage. In system, recognize pathogen-associated molecular patterns carried by microorganisms activate host’s innate response. Furthermore, bacterial lead to oxidative stress through multiple signaling pathways. Many studies revealed serves dual functions: moderate help clear invaders maintain sperm motility, but excessive will induce host Additionally, is always accompanied autophagy which also homeostasis. Male homeostasis disequilibrium inflammation genitourinary influence spermatogenesis, even infertility. Here, we focus on effect infection in explore crosslink between during this process.

Language: Английский

Citations

32
Role of Macroautophagy in Mammalian Male Reproductive Physiology DOI Creative Commons
Doaa Kirat, Ahmed Mohamed Alahwany, Ahmed Hamed Arisha

et al.

Cells, Journal Year: 2023, Volume and Issue: 12(9), P. 1322 - 1322

Published: May 5, 2023

Physiologically, autophagy is an evolutionarily conserved and self-degradative process in cells. Autophagy carries out normal physiological roles throughout mammalian life. Accumulating evidence shows as a mechanism for cellular growth, development, differentiation, survival, homeostasis. In male reproductive systems, spermatogenesis steroidogenesis need balance between degradation energy supply to preserve metabolic The main of includes the formation maturation phagophore, autophagosome, autolysosome. controlled by group autophagy-related genes that form core machinery autophagy. Three types mechanisms have been discovered cells: macroautophagy, microautophagy, chaperone-mediated classified non-selective or selective. Non-selective macroautophagy randomly engulfs cytoplasmic components autophagosomes are degraded lysosomal enzymes. While selective precisely identifies degrades specific element, current findings shown novel functional reproduction. It has recognized dysfunction can be associated with infertility. Overall, this review provides overview molecular basics summarizes latest on key role physiology.

Language: Английский

Citations

12
Antioxidants and Oxidants in Boar Spermatozoa and Their Surrounding Environment Are Associated with AMPK Activation during Liquid Storage DOI Creative Commons
Junwei Li, Wenming Zhao,

Jiaqiao Zhu

et al.

Veterinary Sciences, Journal Year: 2023, Volume and Issue: 10(3), P. 214 - 214

Published: March 10, 2023

Activation of the AMP-activated protein kinase (AMPK) has been demonstrated to be beneficial for boar sperm quality and functionality, while underlying mechanism AMPK activation spermatozoa remains obscure. This study aimed explore effect antioxidants oxidants in their surrounding fluid (SF) on during liquid storage. Ejaculates from Duroc boars, routinely used semen production, were collected diluted a final concentration 25 × 106/mL. In experiment 1, twenty-five samples eighteen boars stored at 17 °C 7 days. 2, three pooled created nine ejaculates used, each sample was treated with 0, 0.1, 0.2, 0.4 μM/L H2O2 3 h. Sperm SF, intracellular AMP/ATP ratio, expression levels phosphorylated (Thr172) determined. significantly decreased storage time terms viability (p < 0.05). Antioxidant oxidant markedly affected time, decline SF total antioxidant capacity (TAC) 0.05), malondialdehyde (MDA) sperm's status (TOS), as well fluctuation superoxidase dismutase-like (SOD-like) activity The ratio increased 0.05) day 4 subsequently its lowest value days 6 2 Correlation analyses indicate that correlated which phosphorylation Treatment induced damages (SF TAC, p 0.05; SOD-like activity, 0.01), an increase MDA, ROS higher comparison control. results suggest are involved

Language: Английский

Citations

11
An important role for triglyceride in regulating spermatogenesis DOI Creative Commons

Charlotte F. Chao,

Yanina‐Yasmin Pesch,

Huaxu Yu

et al.

eLife, Journal Year: 2024, Volume and Issue: 12

Published: May 28, 2024

Drosophila is a powerful model to study how lipids affect spermatogenesis. Yet, the contribution of neutral lipids, major lipid group which resides in organelles called droplets (LD), sperm development largely unknown. Emerging evidence suggests LD are present testis and that loss lipid- LD-associated genes causes subfertility; however, key regulators remain unclear. Here, we show early-stage somatic germline cells within testis. We identified role for triglyceride lipase brummer ( bmm ) regulating LD, found whole-body leads defects development. Importantly, these represent cell-autonomous roles Because lipidomic analysis mutants revealed excess accumulation, spermatogenic were rescued by genetically blocking synthesis, our data suggest -mediated regulation influences This identifies as an important contributes development, reveals levels during

Language: Английский

Citations

4
Metformin and Rosiglitazone affect motility, lipid peroxidation and mitochondrial activity of thawed equine spermatozoa DOI
Alexandra Úsuga, Ana Isabel Vergara Iraeta,

Manuela Tobón

et al.

Journal of Equine Veterinary Science, Journal Year: 2025, Volume and Issue: unknown, P. 105570 - 105570

Published: April 1, 2025

Language: Английский

Citations

0
Impact of metformin on gene expression in Burmese loach (Lepidocephalichthys berdmorei) from Manipur, India DOI

Pratima Ningombam,

Rita Kundu, Semanti Ghosh

et al.

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 978, P. 179458 - 179458

Published: April 19, 2025

Language: Английский

Citations

0
The effect of Nigella sativa oil and metformin on male seminal parameters and testosterone in Wistar rats exposed to an obesogenic diet DOI Open Access
Kristian Leisegang,

Walid Almaghrawi,

Ralf Henkel

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2020, Volume and Issue: 133, P. 111085 - 111085

Published: Dec. 9, 2020

Obesity is a significant global health and socio-economic challenge, considered an important risk factor for poor outcomes including male reproductive dysfunction infertility. As excess adiposity causes testicular infertility, novel therapeutic strategies require investigation. Nigella sativa (Ns) seed oil metformin have both demonstrated potential positive effect on obesity, although remain poorly investigated in fertility. Therefore, this study aimed to determine the of Ns total body weight (TBW), mitochondrial membrane (MMP), serum testosterone semen parameters obese animal model. Wistar rats (n = 54) were divided into six groups: normal chow (NC), high sugar diet (HSD) only, HSD saline, (75 mg/Kg/day), (200 mg/Kg/day) (NS200), (400 (NS400). Intervention was force fed last 8 weeks 14 dietary exposures. Results showed that increased TBW (P 0.001) reduced sperm concentration 0.013) progressive motility 0.009) compared NC group. Metformin, NS200 NS400 improved 0.035, P 0.006 0.005, respectively) < saline group, where 0.001 0.049, MMP 0.001). There no changes viability all experimental exposures, 0.047) negatively affected viability. Metformin may be treatment options obesity-induced negative impact cautioned dose Ns. These results warrant further investigation management infertile males.

Language: Английский

Citations

31
Improving the post-thaw quality of rooster semen using the extender supplemented with resveratrol DOI Creative Commons

Fereshteh Sadat Rezaie,

Maryam Hezavehei, Mohsen Sharafi

et al.

Poultry Science, Journal Year: 2021, Volume and Issue: 100(9), P. 101290 - 101290

Published: May 27, 2021

Avian spermatozoa are highly susceptible to reactive oxygen species (ROS) produced during the cryopreservation. The aim of current study was investigate antioxidant effects resveratrol (RSV) rooster semen Changes in expression AMP-activated protein kinase as a possible mechanism behind beneficial were also evaluated. Semen samples collected from ten Ross broiler breeders (52-wk) using abdominal massage, then divided into 4 equal aliquots and cryopreserved Beltsville extender that contained different concentrations (0 µM, 0.01µM, 0.1µM, 1µM) RSV. higher percentage (P < 0.05) total motility membrane integrity observed RSV-0.1 compared other frozen groups. Moreover, sperm mitochondrial activity RSV-0.01 control 0.05). lowest apoptotic like changes found comparison groups RSV-1 levels H2O2 O2− groups, respectively. Malondialdehyde (MDA) concentration, velocity average path (VAP), linearity (LIN) not affected by RSV > We dose-dependent increase exposed Thus, increased phosphorylation but had no positive on post thaw parameters. Our findings suggest improve thawed functions, these might be mediated through activation kinase.

Language: Английский

Citations

25