BMC Musculoskeletal Disorders,
Journal Year:
2025,
Volume and Issue:
26(1)
Published: March 22, 2025
Adipogenesis
is
intricately
linked
to
the
onset
and
progression
of
muscle
aging;
however,
relevant
biomarkers
remain
unclear.
This
study
sought
identify
key
genes
associated
with
adipogenesis
in
context
aging.
Firstly,
gene
expression
profiles
from
biopsies
vastus
lateralis
both
young
elderly
population
were
retrieved
GEO
database.
After
intersecting
results
differential
analysis,
weighted
co-expression
network
sets
adipogenesis-related
genes,
29
expressed
(ARDEGs)
selected.
Connectivity
Map
(cMAP)
analysis
identified
tamsulosin,
fraxidin,
alaproclate
as
target
compounds.
In
further,
using
three
machine
learning
algorithms
friends
four
hub
ARDEGs,
ESRRA,
RXRG,
GADD45A,
CEBPB
verified
vivo
aged
mice
muscles.
Immune
infiltration
showed
a
strong
link
between
several
immune
cells
ARDEGs.
all,
these
findings
suggested
that
could
serve
related
Journal of Translational Medicine,
Journal Year:
2022,
Volume and Issue:
20(1)
Published: Aug. 2, 2022
Abstract
Doxorubicin
(Dox)
is
the
standard
treatment
approach
for
osteosarcoma
(OS),
while
acquired
drug
resistance
seriously
attenuates
its
efficiency.
The
present
study
aimed
to
investigate
potential
roles
of
metabolic
reprogramming
and
related
regulatory
mechanism
in
Dox-resistant
OS
cells.
results
showed
that
ATP
levels,
lactate
generation,
glucose
consumption
oxygen
rate
were
significantly
increased
cells
compared
with
parental
Furthermore,
revealed
expression
estrogen-related
receptor
alpha
(ERRα)
was
involved
chemotherapy
resistant
cells,
since
targeted
inhibition
ERRα
restored
shifting
profiles.
Mechanistic
analysis
indicated
mRNA
stability,
rather
than
transcription
markedly
chemoresistant
Therefore,
it
hypothesized
3ʹ-untranslated
region
methylated
by
N
6
-methyladenine,
which
could
further
recruit
insulin-like
growth
factor
2
binding
protein
1
(IGF2BP1)
suppress
decay
increase
stability.
IGF2BP1
knockdown
downregulated
reversed
alteration
Additionally,
oncogenic
effect
IGF2BP1/ERRα
axis
on
verified
vitro
vivo
experiments.
Clinical
also
levels
associated
clinical
progression
OS.
Collectively,
current
suggested
regulate
contribute
chemoresistance
European Journal of Organic Chemistry,
Journal Year:
2023,
Volume and Issue:
26(19)
Published: March 29, 2023
Abstract
Thiazolidinedione
(TZD)
is
one
of
the
privileged
heterocyclic
rings
and
has
shown
many
biological
applications
in
medicinal
chemistry
drug
discovery.
This
review
covers
synthetic
approaches
TZD
its
derivatives,
different
techniques
for
affording
desired
regioselectivity
stereoselectivity,
that
would
enhance
reaction
conditions
such
as
microwave,
one‐pot,
or
ultrasound
synthesis.
It
focuses
on
challenges
glitazones
transformation
other
heterocycles
to
TZD.
Moreover,
chemical
behavior
through
substitution
N3
position,
modification
C5
annealing
complex
systems,
hybridization
with
pharmacologically
attractive
moieties
are
discussed.
All
reactions
mentioned
provided
possible
conditions,
mechanisms,
derivatives
scope,
yield
clarified
by
construction
potential
agents.
The
also
answers
questions
about
rapid
racemization
glitazones,
their
toxicity,
considering
pan‐assay
interference
compounds
(PAINS)
not,
influence
saturation
5‐position
activities.
a
comprehensive
guide
make
informed
decisions
potentials.
Autophagy,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 12, 2025
Protein
translation
is
an
energy-intensive
ribosome-driven
process
that
reduced
during
nutrient
scarcity
to
conserve
cellular
resources.
During
prolonged
starvation,
cells
selectively
translate
specific
proteins
enhance
their
survival
(adaptive
translation);
however,
this
poorly
understood.
Accordingly,
we
analyzed
protein
and
mRNA
transcription
by
multiple
methods
in
vitro
vivo
investigate
adaptive
hepatic
starvation.
While
acute
starvation
suppressed
general,
proteomic
analysis
showed
induced
of
lysosome
autolysosome
proteins.
Significantly,
the
expression
orphan
nuclear
receptor,
ESRRA
(estrogen
related
alpha)
increased
served
as
a
master
regulator
transcriptionally
stimulating
Rplp1
(ribosomal
lateral
stalk
subunit
P1)
gene
expression.
Overexpression
or
siRNA
knockdown
Esrra
led
parallel
changes
expression,
macroautophagy/autophagy
translation,
autophagy
activity.
Remarkably,
have
found
had
dual
functions
not
only
regulating
but
also
controlling
via
ESRRA-RPLP1-lysosome-autophagy
pathway
BMC Musculoskeletal Disorders,
Journal Year:
2025,
Volume and Issue:
26(1)
Published: March 22, 2025
Adipogenesis
is
intricately
linked
to
the
onset
and
progression
of
muscle
aging;
however,
relevant
biomarkers
remain
unclear.
This
study
sought
identify
key
genes
associated
with
adipogenesis
in
context
aging.
Firstly,
gene
expression
profiles
from
biopsies
vastus
lateralis
both
young
elderly
population
were
retrieved
GEO
database.
After
intersecting
results
differential
analysis,
weighted
co-expression
network
sets
adipogenesis-related
genes,
29
expressed
(ARDEGs)
selected.
Connectivity
Map
(cMAP)
analysis
identified
tamsulosin,
fraxidin,
alaproclate
as
target
compounds.
In
further,
using
three
machine
learning
algorithms
friends
four
hub
ARDEGs,
ESRRA,
RXRG,
GADD45A,
CEBPB
verified
vivo
aged
mice
muscles.
Immune
infiltration
showed
a
strong
link
between
several
immune
cells
ARDEGs.
all,
these
findings
suggested
that
could
serve
related
