Nature Communications,
Journal Year:
2023,
Volume and Issue:
14(1)
Published: Nov. 22, 2023
Abstract
While
mesenchymal
stem
cells
(MSCs)
have
gained
enormous
attention
due
to
their
unique
properties
of
self-renewal,
colony
formation,
and
differentiation
potential,
the
MSC
secretome
has
become
attractive
its
roles
in
immunomodulation,
anti-inflammatory
activity,
angiogenesis,
anti-apoptosis.
However,
precise
stimulation
efficient
production
for
therapeutic
applications
are
challenging
problems
solve.
Here,
we
report
on
Acoustofluidic
Interfaces
Mechanobiological
Secretome
MSCs:
AIMS.
We
create
an
acoustofluidic
mechanobiological
environment
form
reproducible
three-dimensional
aggregates,
which
produce
with
high
efficiency.
confirm
increased
is
improved
cell-cell
interactions
using
AIMS:
key
mediator
N-cadherin
was
up-regulated
while
functional
blocking
resulted
no
enhancement
secretome.
After
being
primed
by
IFN-γ,
profile
aggregates
contains
more
cytokines
can
be
used
inhibit
pro-inflammatory
response
M1
phenotype
macrophages,
suppress
T
cell
activation,
support
B
functions.
As
such,
modified
personalized
secretome-based
therapies.
AIMS
acts
as
a
powerful
tool
improving
precisely
tuning
secretory
develop
new
treatments
translational
medicine.
Neural Regeneration Research,
Journal Year:
2023,
Volume and Issue:
19(1), P. 49 - 54
Published: April 13, 2023
Traumatic
brain
injury
is
a
serious
and
complex
neurological
condition
that
affects
millions
of
people
worldwide.
Despite
significant
advancements
in
the
field
medicine,
effective
treatments
for
traumatic
remain
limited.
Recently,
extracellular
vesicles
released
from
mesenchymal
stem/stromal
cells
have
emerged
as
promising
novel
therapy
injury.
Extracellular
are
small
membrane-bound
naturally
by
cells,
including
those
brain,
can
be
engineered
to
contain
therapeutic
cargo,
such
anti-inflammatory
molecules,
growth
factors,
microRNAs.
When
administered
intravenously,
cross
blood-brain
barrier
deliver
their
cargos
site
injury,
where
they
taken
up
recipient
modulate
inflammatory
response,
promote
neuroregeneration,
improve
functional
outcomes.
In
preclinical
studies,
vesicle-based
therapies
shown
results
promoting
recovery
after
reducing
neuronal
damage,
improving
cognitive
function,
enhancing
motor
recovery.
While
further
research
needed
establish
safety
efficacy
humans,
represent
approach
treatment
this
review,
we
summarize
cell-derived
cell-free
via
neuroprotection
neurorestoration
brain-derived
potential
biofluid
biomarkers
large
animal
models
Asian Journal of Medicine and Health,
Journal Year:
2024,
Volume and Issue:
22(3), P. 49 - 61
Published: March 1, 2024
The
wound
healing
process
is
an
intricate
biological
phenomenon
that
requires
various
crucial
components,
including
repair
cells,
proteins,
and
factors.
of
can
be
categorized
into
three
interrelated
stages:
the
stage
inflammation,
proliferation,
remodeling.
Any
interruptions
during
this
lead
to
deviates
from
typical
progression.
It
highlight
growth
factors
have
a
profoundly
influential
effect
in
particular
situation.
This
study
conducts
thorough
analytical
literature
review
investigate
essential
role
(TGF-β,
PDGF,
KGF,
FGF,
Pro
Collagen,
VEGF)
healing.
examines
biochemical
molecular
mechanisms
via
which
these
impact
process,
remodeling
phases,
by
analyzing
current
authoritative
scientific
literature.
paper
precisely
outlines
variables,
stem
cell
secretome
enriched
with
such
as
TGF-β,
VEGF,
exogenous
factors,
previously
discussed.
explores
their
on
tissue
regeneration,
angiogenesis,
extracellular
matrix
formation,
components
process.
main
objective
provide
comprehensive
summary
latest
research
findings,
includes
in-depth
analysis
contributions
secretomes
findings
useful
insights
potential
factor-based
treatment
approaches,
highlighting
how
leveraged
enhance
Advanced Healthcare Materials,
Journal Year:
2021,
Volume and Issue:
10(21)
Published: Sept. 5, 2021
Abstract
Cell‐based
approaches
to
tissue
repair
suffer
from
rapid
cell
death
upon
implantation,
limiting
the
window
for
therapeutic
intervention.
Despite
robust
lineage‐specific
differentiation
potential
in
vitro,
function
of
transplanted
mesenchymal
stromal
cells
(MSCs)
vivo
is
largely
attributed
their
potent
secretome
comprising
a
variety
growth
factors
(GFs).
Furthermore,
GF
secretion
markedly
increased
when
MSCs
are
formed
into
spheroids.
Native
GFs
sequestered
within
extracellular
matrix
(ECM)
via
sulfated
glycosaminoglycans,
increasing
potency
signaling
compared
unbound
form.
To
address
critical
need
prolong
efficacy
cells,
alginate
hydrogels
modified
with
sulfate
groups
sequester
endogenous
heparin‐binding
secreted
by
MSC
The
influence
crosslinking
method
and
modification
assessed
on
mechanical
properties,
degradation
rate,
degree
modification.
Sulfated
mixture
MSC‐secreted
biomolecules,
thereby
prolonging
effect
spheroids
regeneration.
longer
durations
retain
bioactivity
regulate
endothelial
tubulogenesis
myoblast
infiltration.
This
platform
has
benefit
serve
as
valuable
tool
investigating
sequestration.
Journal of Multidisciplinary Healthcare,
Journal Year:
2023,
Volume and Issue:
Volume 16, P. 1763 - 1777
Published: June 1, 2023
Chronic
wounds
carry
financial
burdens
and
increase
morbidity
mortality,
especially
in
diabetic
ulcers
Hansen's
Morbus.
More
than
50%
of
chronic
are
difficult
to
heal
with
regular
treatment
require
new
types
therapy
such
as
the
use
secretome
human
umbilical
cord
mesenchymal
stem
cells
(SM-hUCMSC).This
experimental
study
was
carried
out
see
effectiveness
using
SM-hUCMSC
Morbus
four
medical
facilities
(multicentre).
The
level
active
secretion
has
been
measured
by
default
10%
gel,
used
a
intervention.
primary
outcome
is
wound
healing
terms
length,
width,
extent
wound.
secondary
side
effects
2
weeks
after
administration.
Follow-up
visits
will
be
scheduled
at
1
post-treatment.Forty-one
successfully
followed
until
end.
In
patients
ulcers,
mean
ulcer
area
were
1.60
(0,50-13,0),
1.3
(0,5-6,0),
2.21
(0,25-78)
cm
square,
respectively,
before
interventions
(0-12),
0,8
(0-6,0),
(0-72)
square
second
follow-up.
change
between
beginning
end
intervention
significant
(p-value
<0.05).The
gel
topically
proven
effective
accelerating
process
healing,
that
not
present
this
study.
Nature Communications,
Journal Year:
2023,
Volume and Issue:
14(1)
Published: Nov. 22, 2023
Abstract
While
mesenchymal
stem
cells
(MSCs)
have
gained
enormous
attention
due
to
their
unique
properties
of
self-renewal,
colony
formation,
and
differentiation
potential,
the
MSC
secretome
has
become
attractive
its
roles
in
immunomodulation,
anti-inflammatory
activity,
angiogenesis,
anti-apoptosis.
However,
precise
stimulation
efficient
production
for
therapeutic
applications
are
challenging
problems
solve.
Here,
we
report
on
Acoustofluidic
Interfaces
Mechanobiological
Secretome
MSCs:
AIMS.
We
create
an
acoustofluidic
mechanobiological
environment
form
reproducible
three-dimensional
aggregates,
which
produce
with
high
efficiency.
confirm
increased
is
improved
cell-cell
interactions
using
AIMS:
key
mediator
N-cadherin
was
up-regulated
while
functional
blocking
resulted
no
enhancement
secretome.
After
being
primed
by
IFN-γ,
profile
aggregates
contains
more
cytokines
can
be
used
inhibit
pro-inflammatory
response
M1
phenotype
macrophages,
suppress
T
cell
activation,
support
B
functions.
As
such,
modified
personalized
secretome-based
therapies.
AIMS
acts
as
a
powerful
tool
improving
precisely
tuning
secretory
develop
new
treatments
translational
medicine.
