Detection and Imaging of the Plant Pathogen Response by Near‐Infrared Fluorescent Polyphenol Sensors

Angewandte Chemie International Edition, Journal Year: 2021, Volume and Issue: 61(2)

Published: Oct. 6, 2021

Abstract Plants use secondary metabolites such as polyphenols for chemical defense against pathogens and herbivores. Despite their importance in plant pathogen interactions tolerance to diseases, it remains challenging detect complex tissues. Here, we create molecular sensors polyphenol imaging that are based on near‐infrared (NIR) fluorescent single‐wall carbon nanotubes (SWCNTs). We identified polyethylene glycol–phospholipids render (6,5)‐SWCNTs sensitive (K d =90 nM) (tannins, flavonoids, …), which red‐shift (up 20 nm) quench emission (ca. 1000 nm). These report changes total level after herbivore or challenge crop systems (Soybean Glycine max) leaf tissue extracts (Tococa spp.). furthermore demonstrate remote of pathogen‐induced release from roots soybean seedlings over the time course 24 h. This approach allows situ visualization understanding real paves way phenotyping optimized secretion.

Language: Английский

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Dying in the Sun: Direct evidence for elevated UV-B radiation at the end-Permian mass extinction

Science Advances, Journal Year: 2023, Volume and Issue: 9(1)

Published: Jan. 6, 2023

Land plants can adjust the concentration of protective ultraviolet B (UV-B)–absorbing compounds (UACs) in outer wall their reproductive propagules response to ambient UV-B flux. To infer changes radiation flux at Earth’s surface during end-Permian mass extinction, we analyze UAC abundances ca. 800 pollen grains from an independently dated Permian-Triassic boundary section Tibet. Our data reveal excursion UACs that coincide with a spike mercury and negative carbon-isotope latest Permian deposits, suggesting close temporal link between large-scale volcanic eruptions, global carbon cycle perturbations, ozone layer disruption. Because enhanced exacerbate environmental deterioration induced by massive magmatism, depletion is considered compelling ecological driver for terrestrial extinction.

Language: Английский

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Multi-omics assisted breeding for biotic stress resistance in soybean

Molecular Biology Reports, Journal Year: 2023, Volume and Issue: 50(4), P. 3787 - 3814

Published: Jan. 24, 2023

Language: Английский

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Beyond Bites: Differential Role of Fall Armyworm Oral Secretions and Saliva in Modulating Sorghum Defenses

Molecular Plant-Microbe Interactions, Journal Year: 2024, Volume and Issue: 37(3), P. 232 - 238

Published: Jan. 19, 2024

Flavonoids are major plant secondary metabolites that provide defense against several insect pests. Previously, it has been shown sorghum ( Sorghum bicolor) flavonoids required for providing resistance to fall armyworm (FAW; Spodoptera frugiperda), which is an important chewing pest on crops. We demonstrate here the role of FAW oral cues in modulating flavonoid defenses. While feeding, insects release two kinds cues: secretions (OS)/regurgitant and saliva. Our results indicate OS induced expression genes related biosynthesis total flavonoids, thereby enhancing sorghum's larvae. Conversely, saliva suppressed flavonoid-based defenses promoted caterpillar growth, independent salivary component, glucose oxidase (GOX). Thus, we infer different may have contrasting roles altering These findings expand our understanding precise modes action help designing novel management strategies this vital cereal crop. [Formula: see text] Copyright © 2024 The Author(s). This open access article distributed under CC BY-NC-ND 4.0 International license .

Language: Английский

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The impact of insect egg deposition on Pinus sylvestris transcriptomic and phytohormonal responses to larval herbivory

Tree Physiology, Journal Year: 2024, Volume and Issue: 44(2)

Published: Jan. 16, 2024

Abstract Plants can improve their resistance to feeding damage by insects if they have perceived insect egg deposition prior larval feeding. Molecular analyses of these egg-mediated defence mechanisms until now focused on angiosperm species. It is unknown how the transcriptome a gymnosperm species responds eggs and subsequent Scots pine (Pinus sylvestris L.) known its defences against larvae herbivorous sawfly Diprion pini L. it has previously received eggs. Here, we analysed transcriptomic phytohormonal responses needles D. (E-pine), (F-pine) both (EF-pine). Pine showed strong and—as expected—to Many egg-responsive genes were also differentially expressed in response damage, play an important role biological processes related cell wall modification, death jasmonic acid signalling. EF-pine fewer changes than F-pine, whereas EF-treated studied so far more transcriptional initial phase only feeding-damaged F-angiosperms. However, as with EF-angiosperms, higher salicylic concentrations F-pine. Based considerable overlap transcriptomes E- suggest that weaker F-pine compensated strong, egg-induced response, which might result maintained

Language: Английский

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Luteolin and quercetin affect aphid feeding behavior

The European Zoological Journal, Journal Year: 2024, Volume and Issue: 91(1), P. 318 - 331

Published: Jan. 2, 2024

The flavone luteolin and the flavonol quercetin naturally occur in plants, providing some resistance against herbivores. However, despite suggesting potential use of these compounds for plant protection, research on their relationship with aphids is limited. aim our study was to assess effect applying two flavonoids, luteolin, at different concentrations, vitro feeding behavior black bean aphid Aphis fabae Scopoli (Hemiptera: Aphididae). In most events associated stylet activity, differences probing did not vary between control gel those flavonoids. Higher concentrations increased number penetrations, while lower prolonged first probing. addition flavonoids gels reduced passive ingestion. At a concentration 0.1%, completely stopped salivation, 0.01%, it shortened average duration this activity. active ingestion, were observed affecting time activity Lower Increased negatively affected behavior. Quercetin better tolerated by A. fabae. A more noticeable an increase These findings could be applied biotechnological projects aiming develop plants resistant other

Language: Английский

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Integrated view of plant metabolic defense with particular focus on chewing herbivores

Journal of Integrative Plant Biology, Journal Year: 2021, Volume and Issue: 64(2), P. 449 - 475

Published: Dec. 16, 2021

Success of plants largely depends on their ability to defend against herbivores. Since emergence the first voracious consumers, maintained adapting structures and chemistry escape from extinction. The constant pressure was further accelerated by adaptation herbivores plant defenses, which all together sparked rise a chemical empire comprised thousands specialized metabolites currently found in plants. Metabolic diversity kingdom is truly amazing, although many have already been identified, large number potentially useful chemicals remain unexplored bio-resources. Similarly, biosynthetic routes for involve enzymes, some still wait identification biochemical characterization. Moreover, regulatory mechanisms that control gene expression enzyme activities metabolism are scarcely known. Finally, understanding how defense exert toxicity and/or repellency remains limited typical examples, such as proteinase inhibitors, cyanogenic compounds nicotine. In this review, we attempt summarizing current status quo metabolic predominantly based survey ubiquitous examples interactions with chewing

Language: Английский

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Flavonoids Are Intra- and Inter-Kingdom Modulator Signals

Microorganisms, Journal Year: 2022, Volume and Issue: 10(12), P. 2479 - 2479

Published: Dec. 15, 2022

Flavonoids are a broad class of secondary metabolites with multifaceted functionalities for plant homeostasis and involved in facing both biotic abiotic stresses to sustain growth health. Furthermore, they were discovered as mediators networking the surrounding environment, showing surprising ability perform signaling compounds multitrophic inter-kingdom level communication that influences host at phytobiome scale. orchestrate plant-neighboring allelopathic interactions, recruit beneficial bacteria mycorrhizal fungi, counteract pathogen outbreak, influence soil microbiome affect physiology improve its resilience fluctuating environmental conditions. This review focuses on diversified spectrum flavonoid functions plants under variety modulation morphogenesis response clues, well their role molecules micro- macroorganisms. Regarding latter, addresses flavonoids key phytochemicals human diet, considering abundance fruits edible plants. Recent evidence highlights nutraceuticals, probiotics promising new drugs treatment several pathologies.

Language: Английский

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Sorghum and maize flavonoids are detrimental to growth and survival of fall armyworm Spodoptera frugiperda

Journal of Pest Science, Journal Year: 2022, Volume and Issue: 96(4), P. 1551 - 1567

Published: July 7, 2022

Language: Английский

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Perspectives for integrated insect pest protection in oilseed rape breeding

Theoretical and Applied Genetics, Journal Year: 2022, Volume and Issue: 135(11), P. 3917 - 3946

Published: March 16, 2022

In the past, breeding for incorporation of insect pest resistance or tolerance into cultivars use in integrated management schemes oilseed rape/canola (Brassica napus) production has hardly ever been approached. This largely due to broad availability insecticides and complexity dealing with high-throughput phenotyping performance plant damage parameters. However, recent changes political framework many countries demand future sustainable crop protection which makes approaches as a measure control attractive again. At same time, new camera-based tracking technologies, knowledge-based genomic technologies scientific insights ecology insect-Brassica interactions are becoming available. Here we discuss prioritise promising strategies direct indirect targets, their time-perspective realisation rape. conclusion, researchers rape breeders can nowadays benefit from an array combination will accelerate development improved multiple resistances/tolerances near future.

Language: Английский

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