Acta Biomaterialia, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 1, 2024
Language: Английский
Acta Biomaterialia, Journal Year: 2025, Volume and Issue: unknown
Published: May 1, 2025
Language: Английский
Citations
0
Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: May 2, 2024
Abstract Exocrine and endocrine pancreas are interconnected anatomically functionally, with vasculature facilitating bidirectional communication. Our understanding of this network remains limited, largely due to two-dimensional histology missing combination three-dimensional imaging. In study, a multiscale 3D-imaging process was used analyze porcine pancreas. Clinical computed tomography, digital volume micro-computed tomography Synchrotron-based propagation-based imaging were applied consecutively. Fields view correlated inversely attainable resolution from whole organism level down capillary structures voxel edge length 2.0 µm. Segmented vascular networks data tissue sections stained by immunohistochemistry revealed highly vascularized regions be intra-islet capillaries islets Langerhans. Generated 3D-datasets allowed for qualitative quantitative organ vessel structure analysis. Beyond the method shows potential application across wide range patho-morphology analyses might possibly provide microstructural blueprints biotissue engineering.
Language: Английский
Citations
2
Tomography of Materials and Structures, Journal Year: 2024, Volume and Issue: 6, P. 100038 - 100038
Published: June 29, 2024
Cardiovascular tissues possess a complex microstructure, which remodel and adapt due to ageing diseases. This evolving microstructure is intrinsically linked the tissue's mechanical properties. To better understand how changes in can impact behavior, 4D-contrast-enhanced microCT (4D-CECT) be used (i.e. situ loading combined with 3D microstructural visualization). Since absorption-based CECT requires use of contrast-enhancing staining agents (CESAs), we investigated six different CESAs for their suitability 4D-CECT imaging arterial tissue, considering ability provide good visualization segmentation while ensuring preservation For this purpose, penetration speed, contrast-enhancement, volume change, stiffness change porcine tissue stained CESA solutions were studied. Based on our results, selected 1:2 Hafnium-substituted Wells-Dawson Polyoxometalate as most suited tissue. Phosphotungstic acid (PTA) Lugol iodine Sorensen's buffer (Lugol), despite being reference state-of-the-art having excellent contrast-enhancement properties, only ones that significantly affected properties Additionally, these two solutions, shrinkage was observed, resulting reduction approximately − 12 % PTA 17 Lugol. Finally, it observed speed all exhibited ratio 60–40 from intimal side adventitial side, likely denser packing elastic lamellae towards adventitia. Overall, study offers valuable new insights selecting comparing various (4D-)CECT.
Language: Английский
Citations
2
Transplantation Direct, Journal Year: 2024, Volume and Issue: 10(6), P. e1624 - e1624
Published: May 15, 2024
Failure to close the abdominal wall after intestinal transplantation (ITx) or multivisceral Tx remains a surgical challenge. An attractive method is use of nonvascularized rectus fascia (NVRF) in which both layers donor are used as an inlay patch without vascular anastomosis. How this graft integrates over time unknown. The study aims provide multilevel analysis neovascularization and integration process NVRF.
Language: Английский
Citations
1
Cancers, Journal Year: 2024, Volume and Issue: 16(10), P. 1939 - 1939
Published: May 20, 2024
This work describes a comprehensive study of the vascular tree and perfusion characteristics normal kidney renal cell carcinoma. Methods: Nephrectomy specimens were perfused ex-vivo, regional blood flow was determined by infusion radioactive microspheres. The architecture characterized micronized barium sulphate infusion. Kidneys subsequently sagitally sectioned, autoradiograms obtained to show perfusate in relation adjacent contact X-ray angiograms. Vascular resistance defined tissue compartments quantified, finally, tumor vasculature 3D reconstructed via micro-CT technique. Results that could be distinctly defined, disclosed high cortical flow. peripheral unit whole specimen 0.78 ± 0.40 (
Language: Английский
Citations
1
Journal of Anatomy, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 25, 2024
Abstract Museum specimens are an increasingly important tool for studying global biodiversity. With the advent of diffusible iodine‐based contrast‐enhanced computed tomography (diceCT), researchers can now visualize organism's internal soft tissue anatomy without need physical dissection or other highly destructive sampling methods. However, there many considerations when deciding which method staining to use diceCT produce best gray‐scale contrast facilitating downstream anatomical analyses. The general lack direct comparisons among methodologies make it difficult determine approaches most appropriate their study. Here, we compare performance ethylic‐Lugol's iodine solution with aqueous‐Lugol's across several vertebrate orders assess differential imaging outcomes. We found that is better visualizing muscle attachment bone but provides overall lower between types. Comparatively, aqueous‐based Lugol's high‐contrast types, although more discern. conclude choice methodology largely depends on type data researcher wishes collect, and provide a decision‐based framework assessing (ethylic aqueous) desired results.
Language: Английский
Citations
1
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown
Published: March 10, 2023
Abstract Angiogenesis is essential for skeletal development, bone healing, and regeneration. Improved non-destructive, three-dimensional (3D) imaging of the vasculature within tissue would benefit many research areas, especially implantology engineering. X-ray microtomography (microCT) a well-suited non-destructive 3D technique morphology. For microCT-based detection vessels, it paramount to use contrast enhancement. Limited differences in radiopacity between perfusion agents mineralized make their distinct segmentation problematic have been major drawback this approach. A decalcification step resolves issue but inhibits simultaneous assessment microstructure vascular The problem contrasting becomes further compounded samples with metal implants. This study describes μAngiofil-enhanced visualization small large animal models, without decalcification. We present microvascular murine tibia, metastatic model, pulp chamber, gingiva, periodontal ligaments. In model (minipig), we perform different types vessels hemimandible containing Moreover, show potential dual-energy approach facilitating distinction applied agent. Our manuscript introduces first soft hard tissues vicinity implants model.
Language: Английский
Citations
3
Hearts, Journal Year: 2023, Volume and Issue: 5(1), P. 14 - 28
Published: Dec. 23, 2023
Cardiovascular diseases are the most common cause of death worldwide, and they still have dramatic consequences on patients’ lives. Murine models often used to study anatomical microstructural changes caused by diseases. Contrast-enhanced microfocus computed tomography (CECT) is a new imaging technique for 3D histology biological tissues. In this study, we confirmed nondestructiveness Hf-WD 1:2 POM-based CECT cryogenic (cryo-CECT) image heart in 3D. The influence quality (i.e., acquisition time spatial resolution) was assessed characterization structural constituents: integrity, coronary blood vessels valves. Coronary were visualized segmented murine hearts, allowing us distinguish veins from arteries visualize distribution right artery left main artery. Finally, demonstrate added value imaging, thickness two leaflets mitral valve three cusps aortic This corroborates cryo-CECT compared classical 2D characterize ex vivo properties hearts paves way detailed (micro)structural analyses future cardiovascular disease obtained mice rats.
Language: Английский
Citations
3
Journal of Cardiovascular Development and Disease, Journal Year: 2022, Volume and Issue: 9(11), P. 379 - 379
Published: Nov. 3, 2022
Structural and Doppler velocity data collected from optical coherence tomography have already provided crucial insights into cardiac morphogenesis. X-ray microtomography other ex vivo methods elucidated structural details of developing hearts. However, by itself, no single imaging modality can provide comprehensive information allowing to fully decipher the inner workings an entire organ. Hence, we introduce a specimen-specific correlative multimodal workflow combining OCT micro-CT which is applicable for modeling early chick heart development-a valuable model organism in cardiovascular development research. The image acquisition processing employ common reagents, lab-based imaging, software that free academic use. Our goal step-by-step guide on how implement this demonstrate why those two modalities together potential new insight normal malformations leading congenital disease.
Language: Английский
Citations
5
Computers in Biology and Medicine, Journal Year: 2022, Volume and Issue: 148, P. 105932 - 105932
Published: Aug. 2, 2022
Language: Английский
Citations
4