Magnetofluid-integrated biosensors based on DNase-dead Cas12a for visual point-of-care testing of HIV-1 by an up and down chip DOI
Di Huang,

Yekai Zhao,

Mengjun Fang

et al.

Lab on a Chip, Journal Year: 2023, Volume and Issue: 23(19), P. 4265 - 4275

Published: Jan. 1, 2023

The CRISPR Cas system, as a novel nucleic acid detection tool, is often hindered by cumbersome experimental procedures, complicated reagent transfer processes, and associated aerosol pollution risks. In this study, an integrated platform named "up down chip" was developed, which combined RT-RAA technology for amplification, DNase-dead Cas12a-modified magnetic beads specific recognition of target acid, HRP-TMB chromogenic reaction signal output in different chambers single microfluidic chip. were migrated up-and-down manner between through driving, achieving "sample-in, result-out" mode. By introducing homemade heating box temperature control during the using naked eye or smartphone APP color-based reading, no professional precise instruments required platform. Using platform, highly sensitive HIV-1 genome low 250 copies (CPs) per mL achieved within 100 min while maintaining good performance against common variants well excellent specificity anti-interference ability. addition, compared with qRT-PCR, it also exhibited accuracy 56 spiked plasma samples, indicating its promising potential clinical application.

Language: Английский

Types and Applications of Nicking Enzyme-Combined Isothermal Amplification DOI Open Access
Siyu Cao, Xiaochen Tang,

Tianshu Chen

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(9), P. 4620 - 4620

Published: April 21, 2022

Due to the sudden outbreak of COVID-19 at end 2019, rapid detection has become an urgent need for community clinics and hospitals. The development isothermal amplification technology nucleic acids in field molecular diagnostic point-of-care testing (POCT) gained a great deal attention recent years. Thanks intensive research on nicking enzymes, enzyme-combined promising platform detection. This is novel technique that uses enzymes improve ordinary amplification. It garnered significant interest as it overcomes complexity traditional diagnostics not subject temperature limitations, relying cleavage efficiently amplify targets very short time provide high level efficiency. In years, several types have been developed they shown potential diagnosis, immunodiagnosis, biochemical identification, other fields. However, this kind some disadvantages. review, principles, advantages disadvantages, applications techniques are reviewed prospects these also considered.

Language: Английский

Citations

16
An integrated fluorescent lateral flow assay for multiplex point-of-care detection of four respiratory viruses DOI

Wenji Chen,

Hong Chen, Ye Liu

et al.

Analytical Biochemistry, Journal Year: 2022, Volume and Issue: 659, P. 114948 - 114948

Published: Oct. 8, 2022

Language: Английский

Citations

16
COVID-19-associated cerebral microbleeds in the general population DOI Creative Commons
Malini Vendela Sagar,

Neus Rodeja Ferrer,

Mostafa Mehdipour Ghazi

et al.

Brain Communications, Journal Year: 2024, Volume and Issue: 6(3)

Published: Jan. 1, 2024

Abstract Cerebral microbleeds are frequent incidental findings on brain MRI and have previously been shown to occur in Coronavirus Disease 2019 (COVID-19) cohorts of critically ill patients. We aimed determine the risk having medically indicated compare non-hospitalized COVID-19-infected patients with non-infected controls. In this retrospective case-control study, we included over 18 years age, an a susceptibility-weighted sequence, between 1 January July 2021. Cases were identified based positive reverse transcriptase polymerase chain reaction test for SARS-CoV-2 matched three non-exposed controls, sex, body mass index comorbidities. The number cerebral each scan was determined using artificial intelligence. 73 cases 219 COVID-19 associated significantly greater odds [odds ratio 2.66 (1.23–5.76, 95% confidence interval)], increasingly so when dementia hospitalized excluded. Our indicate that may be infections. This finding add pathophysiological considerations help explain previous COVID-19.

Language: Английский

Citations

2
Diagnostic techniques for COVID-19: A mini-review DOI
Xianyong Wu, Qiming Chen,

LI Junhai

et al.

Journal of Virological Methods, Journal Year: 2021, Volume and Issue: 301, P. 114437 - 114437

Published: Dec. 18, 2021

Language: Английский

Citations

15
Magnetofluid-integrated biosensors based on DNase-dead Cas12a for visual point-of-care testing of HIV-1 by an up and down chip DOI
Di Huang,

Yekai Zhao,

Mengjun Fang

et al.

Lab on a Chip, Journal Year: 2023, Volume and Issue: 23(19), P. 4265 - 4275

Published: Jan. 1, 2023

The CRISPR Cas system, as a novel nucleic acid detection tool, is often hindered by cumbersome experimental procedures, complicated reagent transfer processes, and associated aerosol pollution risks. In this study, an integrated platform named "up down chip" was developed, which combined RT-RAA technology for amplification, DNase-dead Cas12a-modified magnetic beads specific recognition of target acid, HRP-TMB chromogenic reaction signal output in different chambers single microfluidic chip. were migrated up-and-down manner between through driving, achieving "sample-in, result-out" mode. By introducing homemade heating box temperature control during the using naked eye or smartphone APP color-based reading, no professional precise instruments required platform. Using platform, highly sensitive HIV-1 genome low 250 copies (CPs) per mL achieved within 100 min while maintaining good performance against common variants well excellent specificity anti-interference ability. addition, compared with qRT-PCR, it also exhibited accuracy 56 spiked plasma samples, indicating its promising potential clinical application.

Language: Английский

Citations

6