Integrated transcriptomics and metabolomics reveal the mechanism of intestinal damage upon acute patulin exposure in mice DOI Creative Commons
Ting Zhang, Min Yan,

Min Chang

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 276, P. 116270 - 116270

Published: April 3, 2024

Mycotoxin contamination has become a major food safety issue and greatly threatens human animal health. Patulin (PAT), common mycotoxin in the environment, is exposed through chain damages gastrointestinal tract. However, its mechanism of enterotoxicity at genetic metabolic levels remains to be elucidated. Herein, intestinal histopathological biochemical indices, transcriptome, metabolome C57BL/6 J mice different doses PAT were successively assessed, as well toxicokinetics vivo. The results showed that acute exposure induced damaged villi crypts, reduced mucus secretion, decreased SOD GSH-Px activities, enhanced MPO activity small intestine mild damage colon. At transcriptional level, genes affected by dose-dependently altered fluctuated primarily inflammation-related signaling pathways oxidative phosphorylation immune responses amino acids decreased, extensive lipids accumulated Seven jointly two sites. Moreover, changes products GST detected tissue but not colonic tissue, explaining degrees Finally, integrated collectively explained toxicological PAT, which directly colon indirectly. These paint clear panorama after provide valuable information on risk toxic PAT.

Language: Английский

The Mechanism of the Gut-Brain Axis in Regulating Food Intake DOI Open Access

Shouren Li,

Mengqi Liu,

Shixi Cao

et al.

Nutrients, Journal Year: 2023, Volume and Issue: 15(17), P. 3728 - 3728

Published: Aug. 25, 2023

With the increasing prevalence of energy metabolism disorders such as diabetes, cardiovascular disease, obesity, and anorexia, regulation feeding has become focus global attention. The gastrointestinal tract is not only site food digestion absorption but also contains a variety appetite-regulating signals gut-brain peptides, short-chain fatty acids (SCFAs), bile (BAs), bacterial proteins, cellular components produced by gut microbes. While central nervous system (CNS), core appetite regulation, can receive integrate these send instructions to downstream effector organs promote or inhibit body’s behaviour. This review will on axis mechanism behaviour, discussing how peripheral signal sensed CNS via role “first order neural nuclei” in process regulation. Here, elucidation may provide new strategies for future production practises treatment diseases anorexia obesity.

Language: Английский

Citations

19
An exploratory study on the association between serotonin and sleep breathing disorders DOI Creative Commons
Mieszko Więckiewicz, Helena Martynowicz, Gilles Lavigne

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: July 21, 2023

Abstract This exploratory observational study aimed to evaluate whether the blood levels of serotonin and enzymes involved in synthesis are associated with sleep breathing parameters. A total 105 patients were included this study, who subjected single-night polysomnography simultaneous audio–video recordings. Peripheral samples collected estimate serum serotonin, tryptophan hydroxylase 1 (TPH1), aromatic l-amino acid decarboxylase (AADC). Results showed a negative correlation between levels, oxygen desaturation index (ODI) ( p = 0.027), central apnea 0.044) obstructive (OA) 0.032) scores. Blood TPH1 negatively correlated average 0.003) minimal saturation 0.035) positively apnea–hypopnea 0.010), OA 0.049), hypopnea 0.007) tendency sleep-disordered seemed co-occur lower higher levels. Clinical Trial Registration : www.ClinicalTrials.gov , identifier NCT04214561

Language: Английский

Citations

18
Role of the gut–brain axis via the subdiaphragmatic vagus nerve in stress resilience of 3,4-methylenedioxymethamphetamine in mice exposed to chronic restrain stress DOI Creative Commons
Youge Qu, Akifumi Eguchi, Li Ma

et al.

Neurobiology of Disease, Journal Year: 2023, Volume and Issue: 189, P. 106348 - 106348

Published: Nov. 11, 2023

3,4-Methylenedioxymethamphetamine (MDMA) is the most widely used illicit substance worldwide. Nevertheless, recent observational studies demonstrated that lifetime MDMA use among U.S. adults was associated with a lower risk of depression and suicide thoughts. We recently reported gut-brain axis may contribute to MDMA-induced stress resilience in mice. To further explore this, we investigated effects subdiaphragmatic vagotomy (SDV) modulating mice subjected chronic restrain (CRS). Pretreatment (10 mg/kg/day for 14 days) blocked anhedonia-like behavior reduced expression synaptic proteins brain-derived neurotrophic factor prefrontal cortex (PFC) CRS-exposed Interestingly, SDV beneficial on these alterations Analysis gut microbiome revealed four measures α-diversity between sham + CRS group group. Moreover, specific microbes differed vehicle group, differences microbial composition were observed all groups. Untargeted metabolomics analysis showed prevented increase plasma levels three compounds [lactic acid, 1-(2-hydroxyethyl)-2,2,6-tetramethyl-4-piperidinol, 8-acetyl-7-hydroxyvumaline] positive correlations found two abundance several across In conclusion, our data suggest via vagus nerve might MDMA.

Language: Английский

Citations

17
The tryptophan metabolic pathway of the microbiome and host cells in health and disease DOI Creative Commons
Kentaro Miyamoto, Tomohisa Sujino, Takanori Kanai∥

et al.

International Immunology, Journal Year: 2024, Volume and Issue: unknown

Published: June 13, 2024

Abstract The intricate and dynamic tryptophan (Trp) metabolic pathway in both the microbiome host cells highlights its profound implications for health disease. This involves complex interactions between cellular bacteria processes, producing bioactive compounds such as 5-hydroxytryptamine (5-HT) kynurenine derivatives. Immune responses to Trp metabolites through specific receptors have been explored, highlighting role of aryl hydrocarbon receptor inflammation modulation. Dysregulation this is implicated various diseases, Alzheimer’s Parkinson’s mood disorders, neuronal autoimmune diseases multiple sclerosis (MS), cancer. In article, we describe impact 5-HT, Trp, indole, on Furthermore, review microbiome-derived that affect immune contribute maintaining homeostasis, especially an experimental encephalitis model MS.

Language: Английский

Citations

8
Integrated transcriptomics and metabolomics reveal the mechanism of intestinal damage upon acute patulin exposure in mice DOI Creative Commons
Ting Zhang, Min Yan,

Min Chang

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 276, P. 116270 - 116270

Published: April 3, 2024

Mycotoxin contamination has become a major food safety issue and greatly threatens human animal health. Patulin (PAT), common mycotoxin in the environment, is exposed through chain damages gastrointestinal tract. However, its mechanism of enterotoxicity at genetic metabolic levels remains to be elucidated. Herein, intestinal histopathological biochemical indices, transcriptome, metabolome C57BL/6 J mice different doses PAT were successively assessed, as well toxicokinetics vivo. The results showed that acute exposure induced damaged villi crypts, reduced mucus secretion, decreased SOD GSH-Px activities, enhanced MPO activity small intestine mild damage colon. At transcriptional level, genes affected by dose-dependently altered fluctuated primarily inflammation-related signaling pathways oxidative phosphorylation immune responses amino acids decreased, extensive lipids accumulated Seven jointly two sites. Moreover, changes products GST detected tissue but not colonic tissue, explaining degrees Finally, integrated collectively explained toxicological PAT, which directly colon indirectly. These paint clear panorama after provide valuable information on risk toxic PAT.

Language: Английский

Citations

6