Celocia Breeding: Classical and Molecular Approaches

Published: Jan. 1, 2025

Language: Английский

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Genetic Response of Solanum lycopersicum L. (Tomato) to Phytophthora infestans and Aspergillus niger

International Journal of Plant Biology, Journal Year: 2025, Volume and Issue: 16(1), P. 35 - 35

Published: March 14, 2025

There is limited information on the genetic response of tomato cultivars to pathogens. This study investigated mechanism that confers tolerance against Phytophthora infestans (Ph) and Aspergills niger (Asp) infection using different accessions. Also, examined effect Ph Asp morphology chromosome number infected tomatoes. Thirty seed accessions were grown in a screen house, complete randomized design triplicate, evaluated for both pathogens disease severity rating scale 1 (highly tolerant) 6 susceptible), while assessment was performed Carnoy’s protocol. Morphological data 28 characteristics collected an IPGRI descriptor, variance components, advance (GA), heritability estimated treated tomatoes controls R statistical program. Accession NHT0254b, which highly tolerant (1.00 ± 0.00) (1.33 0.58), diploid (2n = 2x 24). The Ph-treated NHT0343a moderately susceptible (3.67 2.31), with 2n 23, NGB00711 (4.33 1.16) 22 after treatment, indicating aneuploidy. In cumulative fruit weight (CFW) had highest environmental (1509.57), seeds (NS) showed genotypic (8.22). NS exhibited (2.97), CFW phenotypic (754.91). Heritability estimates length (63.0%), size core (65.0%), chambers (60.0%) Ph, only (56.0%) terms yield characteristics. Thus, traits are recommended selection improvement.

Language: Английский

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Ornamental Crops: An Introduction to Bulbous Flowers

Published: Jan. 1, 2025

Language: Английский

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Genetic Breeding and Biotechnology Advances in David’s Lily (Lilium davidii Duch. ex Elwes)

Published: Jan. 1, 2025

Language: Английский

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Floral volatile chemical diversity in Hedychium F1 hybrid population

Industrial Crops and Products, Journal Year: 2022, Volume and Issue: 184, P. 115032 - 115032

Published: May 6, 2022

Language: Английский

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The Necrotroph Botrytis cinerea BcSpd1 Plays a Key Role in Modulating Both Fungal Pathogenic Factors and Plant Disease Development

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: June 30, 2022

Botrytis cinerea is a necrotrophic microbe that causes gray mold disease in broad range of hosts. In the present study, we conducted molecular microbiology and transcriptomic analyses host-B. interaction to investigate plant defense response fungal pathogenicity. Upon B. infection, responses changed from activation repression; thus, expression many genes decreased Arabidopsis thaliana. Zn(II)2Cys6 transcription factor BcSpd1 was involved suppression as ΔBcSpd1 altered wild-type B05.10 virulence by recovering part at early infection stage. affected sclerotium development, cushion formation, biosynthesis melanin, change environmental pH values, which were reported influence virulence. Specifically, bound promoter gene encoding quercetin dioxygenase (BcQdo) positively expression, catalyzing antifungal flavonoid degradation. This study indicates plays key role toward plants regulating pathogenicity-related compounds thereby suppressing defense.

Language: Английский

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Host-induced gene silencing of the Verticillium dahliae thiamine transporter protein gene (VdThit) confers resistance to Verticillium wilt in cotton

Journal of Integrative Agriculture, Journal Year: 2024, Volume and Issue: 23(10), P. 3358 - 3369

Published: March 6, 2024

Verticillium wilt (VW), induced by the soil-borne fungus dahliae (Vd), poses a substantial threat to diverse array of plant species. Employing molecular breeding technology for development cotton varieties with heightened resistance VW stands out as one most efficacious protective measures. In this investigation, we successfully generated two stable transgenic lines (Gossypium hirsutum L.) VdThit-RNAi-1 and VdThit-RNAi-2 using host-induced gene silencing (HIGS) introduce double-stranded RNA (dsRNA) targeting thiamine transporter protein (VdThit). Southern blot analysis confirmed presence single-copy insertion in both lines. Microscopic examination disclosed marked reduction colonization spread Vd-mCherry roots VdThit-RNAi compared wild type (WT). Correspondingly, disease index fungal biomass VdThit-RNAi-1/2 exhibited significant decrease. Real-time quantitative PCR (qRT-PCR) demonstrated inhibition VdThit expression following prolonged inoculation cotton. Small sequencing (sRNA-Seq) further revealed generation number VdThit-specific siRNAs Additionally, siVdThit produced resulted elevated multiple genes involved biosynthesis pathway Vd. field conditions, significantly enhanced yield WT. summary, our findings underscore efficacy HIGS restraining infection Vd cotton, thereby potentially expediting promising strategy.

Language: Английский

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Intricacies of plants' innate immune responses and their dynamic relationship with fungi: A review

Microbiological Research, Journal Year: 2024, Volume and Issue: 285, P. 127758 - 127758

Published: May 13, 2024

Language: Английский

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Breeding of ornamental plants in Russia

PLANT BIOTECHNOLOGY AND BREEDING, Journal Year: 2022, Volume and Issue: 4(4), P. 40 - 54

Published: Feb. 16, 2022

The breeding of ornamental plants as a branch crop production is an integral part the set measures aimed at obtaining wide range different with high decorative characteristics. main objective this creation that are attractive to consumer and commercially characterized by such valuable biological features adaptability, resistance diseases, pests, frost others. Most plant varieties were bred means traditional methods selection, distant hybridization, clone breeding, radiation chemically induced mutagenesis. However, use tools limited potential for intraspecific variability. development modern biotechnological genetic approaches new has made it possible modify genotype qualitatively level. present review covers directions in methodology Russia, ways mobilizing resources crops rose, clematis, canna, chrysanthemum, pelargonia, iris, daylily, tulip, lilac, rhododendron. Aslo, offers examples work uderway leading specialized scientific institutions Russian State Agrarian University – K.A. Timiryazev Moscow Agricultural Academy, I.V. Michurin Federal Research Centre, Nikitsky Botanical Gardens National Scientific Center RAS, Subtropical Academy Sciences, Garden M.V. Lomonosov University, All-Russian Institute Tobacco, Mahorka Tobacco Products. world flower markets, problem resolving issue quality import substitution, prospects floriculture Russia foreseeable future also briefly considered.

Language: Английский

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Characterization and control of Rhizoctonia solani affecting lucky bamboo (Dracaena sanderiana hort. ex. Mast.) using some bioagents

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: April 24, 2023

In a survey conducted during the period of March-May 2019 in nurseries, warehouses, and shops at three governorates (Alexandria, El-Behera, Giza governorates, Egypt), symptoms root rot, basal stem wilt disease complex were observed lucky bamboo (Dracaena sanderiana hort. ex. Mast.). The highest infection percentage was found collected from Alexandria City (47.67%), while severity El-Behera Governorate (35.19%). Rhizoctonia solani, Fusarium oxysporum, F. Aspergillus niger, Alternaria alternate isolated identified infected samples. R. solani isolates most dominant among recovered fungal species with 80.89% total (246). Pathogenicity tests showed that pathogen 100% 76.67% severity. Molecular identification characterized isolate as AUMC 15120, MZ723906. Meanwhile, four biological control agents (bioagents) healthy samples based on cultural, morphological, microscopic characteristics, molecular phylogenetic analysis Clonostachys rosea 15121, OL461708; Bacillus circulans TAG1, MW441316; B. siamensis TAP1, MW441318 Ochrobactrum anthropi TAM1, MW441317. bioagents potential inhibition vitro well vivo plants vase treatments compared to untreated inoculated certain fungicides biocides used (Moncut, Rizolex-T, Topsin-M, Bio-Zeid, Bio-Arc). bioagent O. growth (85.11%) colony, which not significantly different biocide Bio-Arc (83.78%). However, C. rosea, values 65.33, 64.44, 60.44%, respectively. On other hand, Bio-Zeid less inhibitory effect (43.11%), lowest recorded by Rizolex-T (34.22%) Topsin-M (28.67%). Furthermore, experiment supported results for effective treatments, where all decreased control. Additionally, effect, i.e., incidence being 13.33% 10%, 75%, respectively, This fungicide Moncut (13.33% 21%) (20% 15%) both parameters, conclusion, MW441317 1 × 108 CFU/ml AUMC15121 107/ml proved be efficient causing rot bamboo, can management without negative impact chemical this is first report isolation pathogenic fungus, biocontrol (Bacillus circulans, siamensis, rosea) associated plants.

Language: Английский

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