Journal of Liposome Research, Journal Year: 2023, Volume and Issue: 34(2), P. 274 - 287
Published: Sept. 23, 2023
Curable approaches for primary osteosarcoma are inadequate and urge investigation of novel therapeutic formulations. Cannabinoid ligands exert antiproliferative apoptotic effect on cells
Language: Английский
Glia, Journal Year: 2022, Volume and Issue: 71(1), P. 44 - 59
Published: July 13, 2022
Abstract The study of the astrocytic contribution to brain functions has been growing in popularity neuroscience field. In last years, and especially since demonstration involvement astrocytes synaptic functions, astrocyte field revealed multiple these cells that seemed inconceivable not long ago. parallel, cannabinoid investigation also identified different ways by which cannabinoids are able interact with cells, modify their alter communication neurons impact behavior. this review, we will describe expression endocannabinoid system members astrocytes. Moreover, relate latest findings regarding modulation some most relevant astroglial namely calcium (Ca 2+ ) dynamics, gliotransmission, metabolism, inflammation.
Language: Английский
Citations
54
PLoS ONE, Journal Year: 2024, Volume and Issue: 19(3), P. e0298697 - e0298697
Published: March 27, 2024
Global cannabis use has risen 23% since 2010, with 209 million reported users, most of whom are males reproductive age. Delta-9-tetrahydrocannabinol (THC), the main psychoactive phytocannabinoid in cannabis, disrupts pro-homeostatic functions endocannabinoid system (ECS) within male system. The ECS is highly involved regulating morpho-functional and intrinsic sperm features that required for fertilization pre-implantation embryo development. Previous work by our group demonstrated THC altered capacitation transcriptome, including several fertility-associated microRNAs (miRs). Despite prevalent among age, clinical pre-clinical research investigating impact paternal on function outcomes artificial technologies (ARTs) remains inconclusive. Therefore, present study investigates vitro exposure functions, contributions to development following IVF. Bovine were used as a translational model human treated concentrations reflect plasma levels after therapeutic (0.032μM), low (0.32μM)-high (4.8μM) recreational use. After 6-hours treatment, did not alter acrosomal reaction, but 4.8μM significantly reduced mitochondrial membrane potential (MMP) (p<0.05), primarily through agonistic interactions CB-receptors. Fertilization bovine oocytes THC-treated developmental rates, blastocysts generated from 0.32–4.8μM had fewer trophoblasts while exposed any concentration cells inner cell mass (ICM), particularly 0.032μM (p<0.001). Fertility associated miRs, miR-346, miR-324, miR-33b, miR-34c analyzed THC-exposed IVF, lower miRs-346, -324, -33b found 0.32μM THC, higher (p<0.05). Levels miR-346 also Our findings suggest may key epigenetic factors This first demonstrate negatively affects quality
Language: Английский
Citations
5
Essays in Biochemistry, Journal Year: 2023, Volume and Issue: 67(1), P. 49 - 61
Published: Jan. 16, 2023
Astrocytes are key players in brain homeostasis and function. During the last years, several studies have cemented this notion by showing that these cells respond to neuronal signals and, via release of molecules modulate support synaptic activity (gliotransmission) participates functions so-called tripartite synapse. Thus, besides their established control metabolism, astrocytes can also actively behavior. Among signaling pathways shape astrocyte, cannabinoid type-1 (CB1) receptor is emerging as a critical player both gliotransmission metabolic cooperation between neurons. In present short review, we describe known newly discovered properties astroglial CB1 receptors role modulating function Based on evidence, finally discuss how mode actions astrocyte might represent clear example inextricable relationship energy metabolism gliotransmission. These tight interactions will need be taken into account for future research call reinforcement theoretical experimental bridges astrocytes.
Language: Английский
Citations
11
Neuron, Journal Year: 2025, Volume and Issue: unknown
Published: April 1, 2025
Language: Английский
Citations
0
Biomedicines, Journal Year: 2022, Volume and Issue: 10(8), P. 1811 - 1811
Published: July 28, 2022
Cannabidiol (CBD) showed anticonvulsant action in several preclinical models and is currently approved by regulatory agencies to treat childhood epilepsy syndromes. However, CBD treatment has limited benefits, its long-term effects on cognition are not fully understood yet. This study aimed examine the impact of pentylenetetrazole (PTZ)-kindling model epilepsy. Adult male Wistar rats (N = 24) received PTZ (35 mg/kg intraperitoneally) every other day until two consecutive generalized seizures occurred. (60 body weight) was administered daily oral route kindled state achieved (n 12). To confirm that formulation administration techniques were concern, liquid chromatography–mass spectrometry performed test brain penetration formula. As a result treatment, lower mortality rate significantly prolonged seizure latency (925.3 ± 120.0 vs. 550.1 69.62 s) observed, while frequency duration influenced. The CBD-treated group significant decrease vertical exploration open field discrimination index novel object recognition (NOR) (−0.01 0.17 0.57 0.15, p 0.04). observed behavioral characteristics may be connected decreased thickness stratum pyramidale or astrogliosis hippocampus. In conclusion, did prevent kindling, nor it affect duration. increase first status epilepticus-related PTZ-kindled rats. cognitive impairment NOR related high dose used this study, which warrant further investigation.
Language: Английский
Citations
16
Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Journal Year: 2024, Volume and Issue: 15(1)
Published: Aug. 3, 2024
As cows transition from pregnancy to lactation, free fatty acids (FFA) are mobilized adipose tissues (AT) through lipolysis counter energy deficits. In clinically healthy cows, intensity is reduced throughout lactation; however, if FFA release exceeds tissue demands or the liver's metabolic capacity, lipid byproducts accumulate, increasing cows' risk of and infectious disease. Endocannabinoids (eCBs) their congeners, N-acylethanolamines (NAEs), lipid-based compounds that modulate metabolism inflammation. Their synthesis depend upon availability precursors abundance synthesizing degrading enzymes transporters. Therefore, we hypothesized eCB production transcription endocannabinoid system components modulated by pathways in adipocytes. To test this hypothesis, stimulated canonical (isoproterenol, 1 µmol/L; ISO) inflammatory (lipopolysaccharide, µg/mL; LPS) adipocytes isolated AT 5 Holstein dairy cows. Following, assessed intensity, adipocytes' eCBs, components. We found ISO LPS at comparable intensities. Exposure either treatment tended elevate eCBs NAEs cultured adipocytes; specific transcriptional profiles differed treatment. On one hand, enhanced 2-arachidonoylglycerol (2-AG) but NAE production. Notably, cells' expression associated with 2-AG biosynthesis (INPP5F, GDPD5, GPAT4), transport (CD36), adipogenesis (PPARG). Conversely, N-arachidonoylethanolamide (AEA). This change coincided NAE-biosynthesizing enzyme, PTPN22, genes related degradation (PTGS2, MGLL, CYP27B1). Furthermore, transporters (HSPA1A, SCP2) anti-adipogenic ion channel, TRPV3. Our data provide evidence for distinct modulatory roles over regulation biosynthesis, degradation, transport, ECS signaling Based on our findings, conclude that, within adipocytes, component are, least part, mediated a pathway-dependent manner. These findings contribute deeper understanding molecular mechanisms underlying AT, potential implications prevention disorders.
Language: Английский
Citations
3
Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(6), P. 813 - 813
Published: June 20, 2024
The enduring relationship between humanity and the cannabis plant has witnessed significant transformations, particularly with widespread legalization of medical cannabis. This led to recognition diverse pharmacological formulations cannabis, containing 545 identified natural compounds, including 144 phytocannabinoids like Δ9-THC CBD. Cannabinoids exert distinct regulatory effects on physiological processes, prompting their investigation in neurodegenerative diseases. Recent research highlights potential modulating protein aggregation mitochondrial dysfunction, crucial factors conditions such as Alzheimer’s Disease, multiple sclerosis, or Parkinson’s disease. discussion emphasizes importance maintaining homeodynamics disorders explores innovative therapeutic approaches nanoparticles RNA aptamers. Moreover, cannabinoids, CBD, demonstrate anti-inflammatory through modulation microglial activity, offering multifaceted neuroprotection mitigating aggregation. Additionally, integration cannabinoids vitamin B12 presents a holistic framework for addressing neurodegeneration, considering roles nervous system functioning hippocampal neurogenesis. synergistic benefits combining CBD underscore promising avenue advancing treatment strategies However, further is imperative fully elucidate applications, emphasizing dynamic nature this field its reshape disease paradigms.
Language: Английский
Citations
2
Current Pharmaceutical Design, Journal Year: 2021, Volume and Issue: 28(32), P. 2603 - 2617
Published: Nov. 29, 2021
Over the past few years, an emerging number of new psychoactive substances (NPSs) entered illicit market. NPSs are designed to resemble effects classical drugs abuse, reinforcing their and duration. Among most abused NPS, synthetic cannabinoids cannabinoid receptor agonists (SCRAs) that mimic effect main psychotropic phytocannabinoid Δ9-tetrahydrocannabinol (THC).We herein reviewed international literature provide available information on newest SCRAs generation.Compared previous generations, structures last generation result in increased affinity for efficacy at CB1 receptors, which thought be mainly responsible THC its analogues. Accordingly, these more potent cannabimimetic may increase adverse reactions such as neurological disorders (e.g., psychosis, agitation, irritability, paranoia, confusion, anxiety), psychiatric episodes hallucinations, delusions, self-harm), other physical conditions tachycardia, hypertension, arrhythmia, chest pain, nausea, vomiting, fever) deaths. In decade, than a hundred from different chemical classes emerged web have been thoroughly studied: they were physico-chemically characterized, pharmaco-toxicological characteristics investigated. The generations include increasingly toxic compounds, posing potential health threat consumers.From November 2017 February 2021, least 20 "fourth-generation" formally reported drug agencies. Our understanding neurotoxicity compounds is still limited due lack global data, but potency toxicity likely higher those generations.
Language: Английский
Citations
16
JSAMS Plus, Journal Year: 2024, Volume and Issue: 4, P. 100073 - 100073
Published: July 16, 2024
Language: Английский
Citations
2
Moscow University Biological Sciences Bulletin, Journal Year: 2024, Volume and Issue: 79(S1), P. S93 - S99
Published: Nov. 22, 2024
Language: Английский
Citations
1