Enzymatic Hydrolysate of Low-Molecular-Weight Soybean Peptides Suppresses Hydrogen Peroxide-Induced Apoptosis of Murine Myoblast C2C12 Cells Possibly via AMPK Activation DOI

Wan Heo,

Jeong Hoon Pan

Journal of Medicinal Food, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

Skeletal muscle health is essential for metabolic homeostasis, and dysregulated apoptosis in cells can lead to wasting degenerative diseases. Although soybean-derived peptides are known their bioactive properties, including antioxidant anti-apoptotic effects, impact on regulation skeletal remains underexplored. This study aims investigate the effects of low-molecular-weight soy peptide hydrolysate (SPH) related markers C2C12 myoblasts. SPH was prepared by enzymatic hydrolysis, its activities were analyzed through vitro assays (i.e., ABTS, DPPH). Cellular studies used evaluate ROS scavenging apoptosis. Results show that enhanced activity, reduced levels, promoted cell proliferation a dose-dependent manner. notably decreased under oxidative stress down-regulating p53, c-Caspase-3, Cyto-c, while promoting HO-1 expression, likely via AMPK activation. Importantly, had no significant inflammation-related proteins or MAPK These findings suggest may have therapeutic potential against intrinsic pathways, contributing maintenance.

Language: Английский

Soy‐derived isoflavones as chemo‐preventive agents targeting multiple signalling pathways for cancer prevention and therapy DOI
Agnieszka Kaufman‐Szymczyk,

Justyna Jalmuzna,

Katarzyna Lubecka

et al.

British Journal of Pharmacology, Journal Year: 2024, Volume and Issue: unknown

Published: March 25, 2024

The chemopreventive and chemotherapeutic properties of soy soy-derived compounds, especially isoflavones, have been extensively studied in recent years. However, contrast to their anticancer effects, such as cell growth inhibition, cycle arrest apoptosis induction, isoflavones also found promote the cancer cells. Therefore, aim this comprehensive review article is present current state knowledge regarding molecular mechanisms by which target multiple cellular signalling pathways Our findings indicate that act as, among other things, potent modulators HOX transcript antisense RNA (HOTAIR)/SWI/SNF-related matrix-associated actin-dependent regulator chromatin subfamily B member 1 (SMARCB1), vascular endothelial factor (VEGF)/C-X-C motif chemokine ligand 12 (CXCL12)/C-X-C receptor type 4 (CXCR4), 17-β-oestradiol (E2)/oestrogen receptor-α (ERα)/neuroglobin (NGB) sonic hedgehog pathways, epigenetic modulatory agents (i.a. miR-155, miR-34a miR-10a-5p) stem cells epithelial-to-mesenchymal transition inhibitors. paper discusses latest epidemiological studies clinical trials provides an insight into extensive research on chemo-preventive therapeutic potential isoflavones.

Language: Английский

Citations

4
Comprehensive review of plant-derived anti-hyperlipidemia peptides: Production, anti-hyperlipidemia mechanism, and structure-activity relationship study DOI
Liuyi Wei, Hao Wu, Xufeng Wang

et al.

Food Chemistry, Journal Year: 2024, Volume and Issue: 461, P. 140715 - 140715

Published: Aug. 2, 2024

Language: Английский

Citations

4
Antioxidant peptides produced by Pediococcus acidilactici YKP4 and Lacticaseibacillus rhamnosus BD2 in fermented soymilk made from germinated soybeans DOI Creative Commons
Hendry Noer Fadlillah, Lilis Nuraida, Azis Boing Sitanggang

et al.

International Journal of Food Science & Technology, Journal Year: 2025, Volume and Issue: 60(1)

Published: Jan. 1, 2025

Abstract Soybeans contain antioxidants and are a rich source of proteins. Germination soybeans improves the bioavailability nutrients reduces content antinutritional compounds. Moreover, lactic acid fermentation high-protein foods, such as soybeans, can produce bioactive peptides. Therefore, this study aimed to peptides through soymilk prepared from germinated using Pediococcus acidilactici YKP4 Lacticaseibacillus rhamnosus BD2 identify antioxidative properties resulting Results demonstrated that <2 kDa fraction soybean milk, fermented by P. L. BD2, had lowest IC50 values 471.16 556.93 μg/ml, respectively. Furthermore, were mainly derived glycinin β-conglycinin. Some identified exhibiting antioxidant activities GKHQQEEENEGGSI, VNPESQQGSPR, IGINAENNQRN, FVDAQPQQKEEG. milk produced act inhibitors dipeptidyl peptidase-4, angiotensin-converting enzyme, peptidase-3.

Language: Английский

Citations

0
Recuperating agricultural wastes into feed additives DOI
Sukhendu Dey,

Megha Santra,

Apurba Ratan Ghosh

et al.

Biocatalysis and Agricultural Biotechnology, Journal Year: 2025, Volume and Issue: 64, P. 103508 - 103508

Published: Jan. 31, 2025

Language: Английский

Citations

0
Enzymatic Hydrolysate of Low-Molecular-Weight Soybean Peptides Suppresses Hydrogen Peroxide-Induced Apoptosis of Murine Myoblast C2C12 Cells Possibly via AMPK Activation DOI

Wan Heo,

Jeong Hoon Pan

Journal of Medicinal Food, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

Skeletal muscle health is essential for metabolic homeostasis, and dysregulated apoptosis in cells can lead to wasting degenerative diseases. Although soybean-derived peptides are known their bioactive properties, including antioxidant anti-apoptotic effects, impact on regulation skeletal remains underexplored. This study aims investigate the effects of low-molecular-weight soy peptide hydrolysate (SPH) related markers C2C12 myoblasts. SPH was prepared by enzymatic hydrolysis, its activities were analyzed through vitro assays (i.e., ABTS, DPPH). Cellular studies used evaluate ROS scavenging apoptosis. Results show that enhanced activity, reduced levels, promoted cell proliferation a dose-dependent manner. notably decreased under oxidative stress down-regulating p53, c-Caspase-3, Cyto-c, while promoting HO-1 expression, likely via AMPK activation. Importantly, had no significant inflammation-related proteins or MAPK These findings suggest may have therapeutic potential against intrinsic pathways, contributing maintenance.

Language: Английский

Citations

0