Ecotoxicology and Environmental Safety,
Journal Year:
2023,
Volume and Issue:
267, P. 115626 - 115626
Published: Oct. 25, 2023
As
the
concerned
emerging
pollutants,
several
lines
of
evidence
have
indicated
that
nanoplastics
(NPs)
lead
to
reproductive
toxicity.
However,
biological
mechanism
underlying
NPs
disturbed
spermatogenesis
remains
largely
unknown.
Therefore,
we
aimed
reveal
potential
impaired
caused
by
long-term
exposure
from
perspective
integrated
metabolome
and
microbiome
analysis.
After
12
weeks
gavage
polystyrene
(PS-NPs)
animo-modified
(Amino-NPs),
a
well-designed
two-exposure
stages
experimental
condition.
We
found
induced
apparent
abnormal
spermatogenesis,
which
appeared
more
severe
in
Amino-NPs
group.
Mechanistically,
14
floras
associated
with
glucose
lipid
metabolism
were
significantly
altered,
as
evidenced
16
S
rRNA
sequencing.
Testicular
revealed
Top
50
changed
metabolites
also
enriched
metabolism.
Subsequently,
combined
gut
analysis
uncovered
strong
correlations
between
Klebsiella,
Blautia,
Parabacteroides,
(e.g.,
PC,
LysoPC
GPCho).
speculate
dysbiosis
microbiota-related
may
be
responsible
for
NPs-induced
damaged
provides
new
insights
into
dysregulated
spermatogenesis.
International Journal of Molecular Sciences,
Journal Year:
2022,
Volume and Issue:
23(7), P. 3718 - 3718
Published: March 28, 2022
Human
spermatozoan
ion
channels
are
specifically
distributed
in
the
membrane,
contribute
to
sperm
motility,
and
associated
with
male
reproductive
abnormalities.
Calcium,
potassium,
protons,
sodium,
chloride
main
ions
that
regulated
across
this
their
intracellular
concentrations
crucial
for
motility.
Fatty
acids
(FAs)
affect
quality
parameters,
pathologies,
fertility,
regulate
channel
functions
other
cells.
However,
date
literature
is
insufficient
draw
any
conclusions
regarding
effects
of
FAs
on
human
channels.
Here,
we
aimed
discern
possible
direct
guidance
future
research.
After
investigating
characteristics
related
modulation
similar
cells
by
FAs,
extrapolated
polyunsaturated
(PUFAs)
have
highest
potency
modulating
increase
Of
PUFAs,
ω-3
unsaturated
fatty
greatest
effect.
We
speculate
saturated
monounsaturated
will
little
no
effect
activity,
though
could
be
opposite
those
considering
differences
between
FA
structure
behavior.
Frontiers in Cell and Developmental Biology,
Journal Year:
2022,
Volume and Issue:
10
Published: June 24, 2022
Cell
lipids
are
differentially
distributed
in
distinct
organelles
and
within
the
leaflets
of
bilayer.
They
can
further
form
laterally
defined
sub-domains
membranes
with
important
signaling
functions.
This
molecular
spatial
complexity
offers
optimal
platforms
for
associated
challenge
dissecting
these
pathways
especially
that
lipid
metabolism
tends
to
be
highly
interconnected.
Lipid
has
historically
been
implicated
gamete
function,
however
detailed
involved
remain
obscure.
In
this
review
we
focus
on
oocyte
sperm
maturation
an
effort
consolidate
current
knowledge
role
set
stage
future
directions.
Agriculture,
Journal Year:
2023,
Volume and Issue:
13(7), P. 1387 - 1387
Published: July 12, 2023
Low-temperature
semen
storage
technologies
are
already
being
used
in
poultry
conservation
programs,
but
the
quality
of
reproductive
material
stored
cryobanks
varies
greatly
and
cannot
always
be
successfully
for
practical
purposes.
Therefore,
it
is
necessary
to
improve
compositions
cryoprotective
media
their
quality.
This
study
aimed
investigate
composition
membrane
lipids
carbohydrates
cytosol
rooster
spermatozoa,
explain
dose-dependent
effect
a
combination
trehalose
fructose
on
preservation
morphological
kinetic
parameters
during
freezing/thawing,
determine
most
effective
diluent
composition.
Ejaculates
were
collected
from
Rhode
Island
Red
roosters
(n
=
10).
The
effectiveness
three
diluents
containing
was
evaluated:
LCM-control
(0
mM),
Treh20
(9.5
Treh30
(13.4
mM).
Chromatographic
analysis
lipids,
carbohydrates,
polyols
spermatozoa
performed.
A
decrease
content
glycolipids
plasma
membranes
2.0%
native
1.1–1.4%
(frozen/thawed)
phospholipids
71.2%
(native)
70.5%
reduced
progressive
sperm
motility
65.7%
12.6–27.6%
(frozen/thawed).
same
dynamics
observed
viability
parameter
90.4%
27.0–41.2%
diluent,
using
(36
mM)
saccharides,
maximally
preserved
lipid
profile
frozen/thawed
which
positively
affected
indicators
general
mobility
viability.
Ecotoxicology and Environmental Safety,
Journal Year:
2023,
Volume and Issue:
267, P. 115626 - 115626
Published: Oct. 25, 2023
As
the
concerned
emerging
pollutants,
several
lines
of
evidence
have
indicated
that
nanoplastics
(NPs)
lead
to
reproductive
toxicity.
However,
biological
mechanism
underlying
NPs
disturbed
spermatogenesis
remains
largely
unknown.
Therefore,
we
aimed
reveal
potential
impaired
caused
by
long-term
exposure
from
perspective
integrated
metabolome
and
microbiome
analysis.
After
12
weeks
gavage
polystyrene
(PS-NPs)
animo-modified
(Amino-NPs),
a
well-designed
two-exposure
stages
experimental
condition.
We
found
induced
apparent
abnormal
spermatogenesis,
which
appeared
more
severe
in
Amino-NPs
group.
Mechanistically,
14
floras
associated
with
glucose
lipid
metabolism
were
significantly
altered,
as
evidenced
16
S
rRNA
sequencing.
Testicular
revealed
Top
50
changed
metabolites
also
enriched
metabolism.
Subsequently,
combined
gut
analysis
uncovered
strong
correlations
between
Klebsiella,
Blautia,
Parabacteroides,
(e.g.,
PC,
LysoPC
GPCho).
speculate
dysbiosis
microbiota-related
may
be
responsible
for
NPs-induced
damaged
provides
new
insights
into
dysregulated
spermatogenesis.
