Whole-body Microbiota of Newborn Calves and Their Response to Prenatal Vitamin and Mineral Supplementation

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: March 21, 2023

ABSTRACT Here, we investigated whether initial microbial colonization at seven different anatomical locations in newborn calves and their blood cytokines are influenced by prenatal vitamin mineral (VTM) supplementation. Samples were collected from the hoof, liver, lung, nasal cavity, eye, rumen (tissue fluid), vagina of beef that born dams received diets with or without VTM supplementation throughout gestation (n=7/group). Calves separated immediately after birth fed colostrum milk replacer until euthanasia 30 h post-initial feeding. The microbiota all samples was assessed using 16S rRNA gene sequencing qPCR. 15 chemokines quantified serum. ocular, respiratory, reproductive sites colonized site-specific differed (0.64 ≥ R 2 0.12, P ≤ 0.003). Only ruminal fluid on ( P< 0.01 Differences <0.05) detected richness (vagina), diversity (ruminal tissue fluid, eye), composition phylum genus level vagina), total bacterial abundance (ocular vagina) between control calves. cytokine IP-10 higher =0.02) Overall, our results suggest despite immediate separation dam upon birth, whole-body 32-h old relatively rich, diverse communities, rumen, oculus seem to be IMPORTANCE Increased appreciation maternal nutrition microbiome’s involvement developmental programming evidence supporting utero highlight factors could impact offspring colonization. any 7 We identified changes ruminal, vaginal, ocular response provided a “holistic” view calf microbiota. Our data obtained same sex age, who dams, hence provides novel insights taxonomic those examined. Combined, this study direction for future work targeting manipulation early life microbiome via alteration harnessing improved cattle health production.

Language: Английский

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Maternal androgen excess inhibits fetal cardiomyocytes proliferation through RB-mediated cell cycle arrest and induces cardiac hypertrophy in adulthood

Journal of Endocrinological Investigation, Journal Year: 2023, Volume and Issue: 47(3), P. 603 - 617

Published: Aug. 29, 2023

Abstract Purpose Maternal hyperandrogenism during pregnancy is associated with adverse gestational outcomes and chronic non-communicable diseases in offspring. However, few studies are reported to demonstrate the association between maternal androgen excess cardiac health This study aimed explore relation exposure utero of offspring fetal adult period. Its underlying mechanism also illustrated this research. Methods Pregnant mice were injected dihydrotestosterone (DHT) from day (GD) 16.5 GD18.5. On GD18.5, heart tissue was collected for metabolite morphological analysis. The hearts qPCR H9c2 cells treated 75 μM androsterone. Immunofluorescence, flow cytometry, qPCR, western blot performed observe cell proliferation mechanism. Results Intrauterine excessive led thinner ventricular wall, decreased number cardiomyocytes caused hypertrophy, compromised function analysis steroid hormone metabolites by ultra performance liquid chromatography tandem mass spectrometry showed that content androsterone significantly increased. Mechanistically, a significant decrease phosphorylated retinoblastoma protein (pRB) cycle-related including cyclin-dependent kinase 2 (CDK2), 4 (CDK4), cyclin D1 (CCND1) cardiomyocytes. resulted cycle arrest at G1–S phase, which turn inhibited cardiomyocyte proliferation. Conclusion Taken together, our results indicate DHT, its could directly through arrest, has life-long-lasting effect on health. Our highlights importance monitoring sex hormones women follow-up high risk intrauterine exposure.

Language: Английский

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Female offspring of mice perinatally exposed to benzophenone-3 showed early subfertility linked to a poor oocyte stockpile

Archives of Toxicology, Journal Year: 2024, Volume and Issue: 98(6), P. 1909 - 1918

Published: March 30, 2024

Language: Английский

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Relationship of Growth Rates to Prenatal Micronutrient Supplementation in Replacement Heifers

Russian Agricultural Sciences, Journal Year: 2024, Volume and Issue: 50(1), P. 81 - 86

Published: Feb. 1, 2024

Language: Английский

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Placental Angiogenesis

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

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