The burden of osteoarthritis: Is it a rising problem?

Best Practice & Research Clinical Rheumatology, Journal Year: 2023, Volume and Issue: 37(2), P. 101836 - 101836

Published: June 1, 2023

Language: Английский

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Integrated multi-omics analyses identify anti-viral host factors and pathways controlling SARS-CoV-2 infection

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Jan. 2, 2024

Host anti-viral factors are essential for controlling SARS-CoV-2 infection but remain largely unknown due to the biases of previous large-scale studies toward pro-viral host factors. To fill in this knowledge gap, we perform a genome-wide CRISPR dropout screen and integrate analyses multi-omics data screen, association studies, single-cell RNA-Seq, host-virus proteins or protein/RNA interactome. This study uncovers many that currently underappreciated, including components V-ATPases, ESCRT, N-glycosylation pathways modulate viral entry and/or replication. The cohesin complex is also identified as an pathway, suggesting important role three-dimensional chromatin organization mediating host-viral interaction. Furthermore, discover another regulator KLF5, transcriptional factor involved sphingolipid metabolism, which up-regulated, harbors genetic variations linked COVID-19 patients with severe symptoms. Anti-viral effects three candidates (DAZAP2/VTA1/KLF5) confirmed individually. Molecular characterization DAZAP2/VTA1/KLF5-knockout cells highlights involvement genes related coagulation system determining severity COVID-19. Together, our results provide further resources understanding network during may help develop new countermeasure strategies.

Language: Английский

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Exploring the Pathophysiology of Long COVID: The Central Role of Low-Grade Inflammation and Multisystem Involvement

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(12), P. 6389 - 6389

Published: June 9, 2024

Long COVID (LC), also referred to as Post COVID-19 Condition, Post-Acute Sequelae of SARS-CoV-2 Infection (PASC), and other terms, represents a complex multisystem disease persisting after the acute phase COVID-19. Characterized by myriad symptoms across different organ systems, LC presents significant diagnostic management challenges. Central disorder is role low-grade inflammation, non-classical inflammatory response that contributes chronicity diversity observed. This review explores pathophysiological underpinnings LC, emphasizing importance inflammation core component. By delineating pathogenetic relationships clinical manifestations this article highlights necessity for an integrated approach employs both personalized medicine standardized protocols aimed at mitigating long-term consequences. The insights gained not only enhance our understanding but inform development therapeutic strategies could be applicable chronic conditions with similar features.

Language: Английский

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Integrative analyses of mendelian randomization and bioinformatics reveal casual relationship and genetic links between COVID-19 and knee osteoarthritis

BMC Medical Genomics, Journal Year: 2025, Volume and Issue: 18(1)

Published: Jan. 2, 2025

Clinical and epidemiological analyses have found an association between coronavirus disease 2019 (COVID-19) knee osteoarthritis (KOA). Infection with COVID-19 may increase the risk of developing KOA. This study aimed to investigate potential causal relationship KOA using Mendelian randomization (MR) explore underlying mechanisms through a systematic bioinformatics approach. Our investigation focused on exploring COVID-19, acute upper respiratory tract infection (URTI) utilizing bidirectional MR Additionally, we conducted differential gene expression analysis public datasets related these three conditions. Subsequent analyses, including transcriptional regulation analysis, immune cell infiltration single-cell druggability evaluation, were performed prioritize therapeutic targets. The results indicate that has one-way impact KOA, while URTI does not play role in this association. Ribosomal dysfunction serve as intermediate factor connecting Specifically, influence metabolic processes extracellular matrix, potentially impacting joint homeostasis. A specific group genes (COL10A1, BGN, COL3A1, COMP, ACAN, THBS2, COL5A1, COL16A1, COL5A2) been identified shared transcriptomic signature response COVID-19. Imatinib, Adiponectin, Myricetin, Tranexamic acid, Chenodeoxycholic acid are drugs for treatment patients uniquely combines tools possibility genetic These findings expected provide novel perspectives biological link

Language: Английский

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Functional Foods: A Promising Strategy for Restoring Gut Microbiota Diversity Impacted by SARS-CoV-2 Variants

Nutrients, Journal Year: 2023, Volume and Issue: 15(11), P. 2631 - 2631

Published: June 5, 2023

Natural herbs and functional foods contain bioactive molecules capable of augmenting the immune system mediating anti-viral functions. Functional foods, such as prebiotics, probiotics, dietary fibers, have been shown to positive effects on gut microbiota diversity function. The use has linked enhanced immunity, regeneration, improved cognitive function, maintenance microbiota, significant improvement in overall health. plays a critical role maintaining health disruptions its balance various problems. SARS-CoV-2 infection affect diversity, emergence variants poses new challenges combat virus. recognizes infects human cells through ACE2 receptors prevalent lung epithelial cells. Humans are prone because their respiratory gastrointestinal tracts rich microbial high levels TMPRSS2. This review article explores potential mitigating impact strategy these effects.

Language: Английский

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Circulating Reelin promotes inflammation and modulates disease activity in acute and long COVID-19 cases

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: June 27, 2023

Thromboembolic complications and excessive inflammation are frequent in severe COVID-19, potentially leading to long COVID. In non-COVID studies, we others demonstrated that circulating Reelin promotes leukocyte infiltration thrombosis. Thus, hypothesized participates endothelial dysfunction hyperinflammation during COVID-19. We showed was increased COVID-19 patients correlated with the disease activity. group, observed a hyperinflammatory state, as judged by concentration of cytokines (IL-1α, IL-4, IL-6, IL-10 IL-17A), chemokines (IP-10 MIP-1β), adhesion markers (E-selectin ICAM-1). level IL-1α, IP-10, MIP-1β, ICAM-1, suggesting specific role for progression. Furthermore, all inflammatory aforementioned returned normal COVID cohort, showing state resolved. Finally, tested inhibition anti-Reelin antibody CR-50 hACE2 transgenic mice infected SARS-CoV-2. prophylactic treatment decreased mortality severity this model. These results demonstrate direct proinflammatory function identify it drug target. This work opens translational clinical applications SARS-CoV-2 infection beyond auto-inflammatory diseases.

Language: Английский

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Circulating levels of inflammatory cytokines and angiogenesis-related growth factors in patients with osteoarthritis after COVID-19

Frontiers in Medicine, Journal Year: 2023, Volume and Issue: 10

Published: July 6, 2023

The disease COVID-19, caused by SARS-CoV-2 infection, has a systemic effect and is associated with number of pathophysiological mechanisms that mobilize wide range biomolecules. Cytokines growth factors (GFs) are critical regulators tissue damage or repair in osteoarthritis (OA) being recognized as key players the pathogenesis COVID-19. A clear understanding long-term consequences especially patients concomitant chronic diseases, limited needs to be elucidated. study aimed evaluate degree inflammation levels pro-angiogenic hypoxic factors, well heat shock proteins HSP60 HSP70 plasma, OA after recovery from COVID-19.The research involved an orthopedic specialty clinic aged 39 80 diagnosed knee OA. All examined were divided into three groups: Control group included conditionally healthy donors, mainly stage II III COVID-19 who had plasma pro-inflammatory molecules IL-1β, IL-6, TNF-α, NF-κB, angiogenic VEGF, FGF-2, PDGF, factor HIF-1α molecular chaperones measured enzyme-linked immunosorbent assay.The showed both groups patients, convalescent there was increase level IL-1β decrease TNF-α NF-κB when compared controls. Systemic deregulation cytokine profile accompanied reduction most pronounced cases VEGF PDGF. This analysis did not reveal any significant difference HIF-1α. stress protein blood OA, those have been established.The results suggest potential role cytokines angiogenesis-related joint pathologies post-COVID-19 disorders.

Language: Английский

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Identification of 3 key genes as novel diagnostic and therapeutic targets for OA and COVID-19

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: May 22, 2023

Background Corona Virus Disease 2019 (COVID-19) and Osteoarthritis (OA) are diseases that seriously affect the physical mental health life quality of patients, particularly elderly patients. However, association between COVID-19 osteoarthritis at genetic level has not been investigated. This study is intended to analyze pathogenesis shared by OA identify drugs could be used treat SARS-CoV-2-infected Methods The four datasets (GSE114007, GSE55235, GSE147507, GSE17111) for analysis in this paper were obtained from GEO database. Common genes identified through Weighted Gene Co-Expression Network Analysis (WGCNA) differential gene expression analysis. least absolute shrinkage selection operator (LASSO) algorithm was screen key genes, which analyzed patterns single-cell Finally, drug prediction molecular docking carried out using Drug Signatures Database (DSigDB) AutoDockTools Results Firstly, WGCNA a total 26 common COVID-19, functional revealed pathological processes changes mainly related immune dysfunction. In addition, we screened 3 DDIT3, MAFF, PNRC1, uncovered possibly involved high neutrophils. established regulatory network free energy binding estimation suitable medicines treatment patients infected with SARS-CoV-2. Conclusion present study, succeeded identifying development both have diagnostic value COVID-19. niclosamide, ciclopirox, ticlopidine found potentially useful

Language: Английский

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The mediating role of physical activity and quality of life in the association between knee osteoarthritis-related symptoms and physical function of post-COVID patients in Metro Manila: a study protocol

BMJ Open, Journal Year: 2025, Volume and Issue: 15(1), P. e088650 - e088650

Published: Jan. 1, 2025

Introduction Knee osteoarthritis (OA) is a chronic condition affecting joint function. Regular physical activity can enhance functional capacity and reduce pain. However, there scarcity of studies relating to knee OA during the COVID-19 pandemic, particularly its impact on symptoms quality life. Methods This analytic cross-sectional study design will recruit participants aged 40 above from Metro Manila with pain history. The Filipino version Injury Osteoarthritis Outcome Score International Physical Activity Questionnaire-Short Form be used assess mediating variables. Analysis employ descriptive regression analyses for data analysis follow Strengthening Reporting Observational Studies in Epidemiology statement reporting data. Ethics dissemination has received ethical approval Review Committee College Rehabilitation Sciences. Study results disseminated through peer-reviewed journal publications conference presentations ensure accessibility healthcare professionals stakeholders, contributing advancement management post-COVID settings.

Language: Английский

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The impact of long COVID on quality of life and work performance among healthcare workers in Portugal

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e19089 - e19089

Published: March 10, 2025

Background Coronavirus disease 2019 (COVID-19) is a multisystem infectious which affected 5.7 million people in Portugal. A subgroup of patients experienced long-term effects from the infection, now referred to as long COVID. Long COVID can considerably reduce quality life (QoL) patients. This study aimed evaluate impact on QoL and work performance among healthcare workers (HCWs) Methods cross-sectional correlational survey was performed 348 HCWs employed either hospitals, or non-hospital health facilities Participants completed an online using Google Forms between May June 2024, consisted multiple-choice questions took less than 10 min fill out. considered present if defined symptoms started at least 3 months after primary infection COVID-19, persisted for 2 months. The outcome measures were QoL. Performance measured seven-point Likert scale evaluated Satisfaction with Life Scale (SWLS). Data analyzed SPSS. Results total 277 participants (79.6%) had history SARS-CoV-2 223 (64.1%) reported Extreme fatigue by 158 (57.0%), cognitive dysfunction 118 (42.6%), shortness breath 76 (27.4%), persistent cough 168 (60.6%). In multivariate analysis, significantly associated lower SWLS scores indicating that negatively impacted Having two more chronic diseases showed trend towards performance, extreme strong negative associations performance. Discussion Based national sample (348 individuals), high percentage These results suggest Portugal have prevalence similar worldwide population. Concerning work, having well dysfunction. Interestingly, we found association being clinical secretary SWLS. might be explained specific challenges stressors faced secretaries, could their conclusion, prevalent studied strongly suggests need targeted care group. this guide authorities addressing important consequences should rehabilitation programs post-COVID-19 recovery.

Language: Английский

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