Emerald Ash Borer Management and Research: Decades of Damage and Still Expanding

Annual Review of Entomology, Journal Year: 2023, Volume and Issue: 69(1), P. 239 - 258

Published: Sept. 14, 2023

Since the discovery of ash tree ( Fraxinus spp.) killer emerald borer (EAB; Agrilus planipennis) in United States 2002 and Moscow, Russia 2003, substantial detection management efforts have been applied to contain monitor its spread mitigate impacts. Despite these efforts, pest continues within North America. It has European Ukraine is causing sporadic outbreaks native range China. The dynamics EAB's expansion events appear be linked lack resistant trees invaded ranges, facilitated by abundance or planted American susceptible species. We review recently gained knowledge EAB; ecological, economic, social impacts; past with their successes limitations. also highlight advances biological control, mechanisms resistance, new approaches under development, aim guiding more effective management.

Language: Английский

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CRISPR/Cas9-gene editing approaches in plant breeding

GM crops & food, Journal Year: 2023, Volume and Issue: 14(1), P. 1 - 17

Published: Sept. 19, 2023

CRISPR/Cas9 gene editing system is recently developed robust genome technology for accelerating plant breeding. Various modifications of this have been established adaptability in varieties as well its improved efficiency and portability. This review provides an in-depth look at the various strategies synthesizing gRNAs efficient delivery cells, including chemical synthesis

Language: Английский

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Advances in lignocellulosic feedstocks for bioenergy and bioproducts

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 1, 2025

Lignocellulose, an abundant renewable resource, presents a promising alternative for sustainable energy and industrial applications. However, large-scale adoption of lignocellulosic feedstocks faces considerable obstacles, including scalability, bioprocessing efficiency, resilience to climate change. This Review examines current efforts future opportunities leveraging in bio-based products, with focus on enhancing conversion efficiency scalability. It also explores emerging biotechnologies such as CRISPR-based genome editing informed by machine learning, aimed at improving feedstock traits reducing the environmental impact fossil fuel dependence. Lignocellulose is produce bioenergy biomaterials. Here, authors review efforts, feedstock-based biomaterials production provide outlook traits.

Language: Английский

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Current status and trends in forest genomics

Forestry Research, Journal Year: 2022, Volume and Issue: 2(1), P. 0 - 0

Published: Jan. 1, 2022

Forests are not only the most predominant of Earth's terrestrial ecosystems, but also core supply for essential products human use. However, global climate change and ongoing population explosion severely threatens health forest ecosystem aggravtes deforestation degradation. Forest genomics has great potential increasing productivity adaptation to changing climate. In last two decades, field advanced quickly owing advent multiple high-throughput sequencing technologies, single cell RNA-seq, clustered regularly interspaced short palindromic repeats (CRISPR)-mediated genome editing, spatial transcriptomes, as well bioinformatics analysis which have led generation multidimensional, multilayered, spatiotemporal gene expression data. These together with basic technologies routinely used in plant biotechnology, enable us tackle many important or unique issues biology, provide a panoramic view an integrative elucidation molecular regulatory mechanisms underlying phenotypic changes variations. this review, we recapitulated advancement current status 12 research branches genomics, then provided future directions focuses each area. Evidently, shift from simple biotechnology-based research, setup investigation interpretation development differentiation just begun emerge.

Language: Английский

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Multi-faceted CRISPR-Cas9 strategy to reduce plant based food loss and waste for sustainable bio-economy – A review

Journal of Environmental Management, Journal Year: 2023, Volume and Issue: 332, P. 117382 - 117382

Published: Feb. 6, 2023

Language: Английский

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Generation of transgene-free canker-resistant Citrus sinensis cv. Hamlin in the T0 generation through Cas12a/CBE co-editing

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: March 26, 2024

Citrus canker disease affects citrus production. This is caused by Xanthomonas citri subsp. (Xcc). Previous studies confirmed that during Xcc infection, PthA4, a transcriptional activator like effector (TALE), translocated from the pathogen to host plant cells. PthA4 binds binding elements (EBEs) in promoter region of susceptibility gene LOB1 (EBE -LOBP) activate its expression and subsequently cause symptoms. Previously, Cas12a/CBE co-editing method was employed disrupt EBE -LOBP pummelo, which highly homozygous. However, most commercial cultivars are heterozygous hybrids more difficult generate homozygous/biallelic mutants. Here, we edit Hamlin ( sinensis ), hybrid cultivar grown worldwide. Binary vector GFP-p1380N-ttLbCas12a:LOBP1-mPBE:ALS2:ALS1 constructed shown be functional via Xcc-facilitated agroinfiltration leaves. construct allows selection transgene-free regenerants GFP, edits ALS chlorsulfuron-resistant as marker for genome editing resulting transient T-DNA nCas9-mPBE:ALS2:ALS1, gene(s) interest (i.e., this study) through ttLbCas12a, thus creating citrus. Totally, 77 plantlets were produced. Among them, 8 transgenic plants (#Ham GFP 1 - #Ham 8), 4 NoGFP 4), rest wild type. plantlets, three lines 1, 2 3) contained biallelic mutations pthA4 , one line 4) had homozygous . We achieved 5.2% mutation efficiency –LOBP C. cv. Hamlin, compared 1.9% pummelo previous study. Importantly, four 3 survived resistant against canker. Taken together, has been successfully used canker‐resistant T0 generation biallelic/homozygous LOB1.

Language: Английский

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Revolutionizing Tree Improvement Through Biotechnology

Published: Jan. 1, 2025

Language: Английский

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Tree population genomics

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 325 - 356

Published: Jan. 1, 2025

Language: Английский

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Multiplex genome editing for climate-resilient woody plants

Frontiers in Forests and Global Change, Journal Year: 2025, Volume and Issue: 8

Published: Feb. 5, 2025

Climate change is severely impacting global forest ecosystems, stressing woody plants due to rising temperatures, shifting precipitation patterns, and extreme weather events. These pressures threaten biodiversity disrupt the essential roles forests play in carbon sequestration, timber production, ecosystem stability. Traditional management strategies, such as selective breeding, cannot keep up with rapid pace of climate change, given long juvenile phase trees. Multiplex genome editing, particularly through CRISPR technologies, offers a promising solution accelerate development climate-resilient traits plants. By simultaneously targeting multiple genes, multiplex enables efficient modification polygenic that govern stress tolerance, disease resistance, other crucial resilience factors. This mini-review examines potential technologies management, agroecological practices, showing how they can improve tree support sustainable forestry response growing challenges change.

Language: Английский

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Current and future challenges to conservation translocations

Published: Feb. 6, 2025

Language: Английский

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