International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(4), P. 1712 - 1712
Published: Feb. 17, 2025
The
role
of
gut
microbiota
has
become
a
research
hotspot
in
recent
years;
however,
whether
the
are
involved
alleviation
or
exacerbation
Deoxynivalenol
(DON)
toxicity
not
been
fully
studied.
Therefore,
objective
this
study
was
to
investigate
reducing
aggravating
intestinal
damage
induced
by
DON
mice.
Mice
that
received
did
receive
antibiotic-induced
flora
clearance
were
orally
given
(5
mg
kg/bw/day)
for
14
days.
At
end
experiment,
serum,
tissue
samples
and
colon
contents
collected
further
analysis.
caused
development
severe
histopathological
damage,
such
as
necrosis
inflammation
jejunum
mice
without
clearance.
levels
tight
junction
proteins
ZO-1
occludin
reduced
In
addition,
mRNA
protein
pro-inflammatory
cytokines
(IL-1β,
IL-6,
TNF-α)
increased
presence
exacerbate
via
changes
abundance
production
damaging
metabolites.
Foods,
Journal Year:
2025,
Volume and Issue:
14(7), P. 1173 - 1173
Published: March 27, 2025
Postbiotics
may
help
strengthen
intestinal
barrier
function.
This
study
assessed
the
effects
of
a
postbiotic
derived
from
Limosilactobacillus
fermentum
and
Lactobacillus
delbrueckii
subsp.
lactis
on
epithelial
cytokine
production.
Human-derived
colon
tubules
were
cultured
chips
for
15
days.
On
day
8,
was
disrupted
with
0.7
μM
afatinib.
Postbiotic
doses
5,
10,
or
20
mg/mL
added
days
6,
11,
13.
Trans-epithelial
electrical
resistance
(TEER)
measured
13,
15,
along
phase
contrast
imaging.
Cytokine
levels
All
three
concentrations
resulted
in
better
TEER
recovery
vs.
control
(p
<
0.001).
10
increased
0.001),
but
only
did
11
0.05).
Phase
imaging
confirmed
dose-dependent
effect.
The
dose
more
effectively
reduced
CCL2,
CX3CL1,
CXCL1,
CXCL5,
IL-8,
IL-11,
IL-4
than
other
0.01),
CXCL10,
IL-10,
IL-23
5
0.01).
In
colonic
organoid
model,
Lactobacillaceae-derived
prevented
drug-induced
damage,
enhanced
recovery,
modulated
secretion
towards
anti-inflammatory
profile
manner.
