Pathology - Research and Practice, Journal Year: 2023, Volume and Issue: 248, P. 154619 - 154619
Published: June 13, 2023
Language: Английский
Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 162, P. 114593 - 114593
Published: March 29, 2023
Multiple sclerosis (MS) is an autoimmune, inflammatory demyelinating disorder of the central nervous system. Accumulating evidence has underscored therapeutic potential bone marrow mesenchymal stem cells (BMSCs)-derived exosomes (BMSC-Exos) containing bioactive compounds in MS. Herein, current study sought to characterize mechanism BMSC-Exos harboring miR-367-3p both BV2 microglia by Erastin-induced ferroptosis and experimental autoimmune encephalomyelitis (EAE), a typical animal model Exosomes were firstly isolated from BMSCs identified for further use. co-cultured with miR-367-3p-containing BMSC-Exos, followed assessment cell ferroptosis. Mechanistic exploration was furthered interaction its downstream regulators. Lastly, injected into EAE mice vivo validation. carrying restrained microglial vitro. Mechanistically, could bind Enhancer zeste homolog 2 (EZH2) restrain EZH2 expression, leading over-expression solute carrier family 7 member 11 (SLC7A11). Meanwhile, SLC7A11 resulted Glutathione Peroxidase 4 (GPX4) activation suppression. Ectopic expression vitro negated protective effects BMSC-Exos. Furthermore, relieved severity suppressing restraining vivo. Collectively, our findings suggest that brings about significant decline repressing alleviating vivo, suggesting possible role overexpression treatment strategy EAE. AVAILABILITY OF DATA AND MATERIALS: The datasets used and/or analyzed during are available corresponding author upon reasonable request.
Language: Английский
Citations
39
International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 723 - 723
Published: Jan. 16, 2025
Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) have emerged as a promising therapeutic strategy for spinal cord injury (SCI). These nanosized possess unique properties such low immunogenicity and the ability to cross biological barriers, making them ideal carriers delivering bioactive molecules injured tissues. MSC-EVs been demonstrated exert multiple beneficial effects in SCI, including reducing inflammation, promoting neuroprotection, enhancing axonal regeneration. Recent studies delved into molecular mechanisms underlying MSC-EV-mediated effects. Exosomal microRNAs (miRNAs) identified key regulators of various cellular processes involved SCI pathogenesis repair. miRNAs can influence oxidative stress, apoptosis by modulating gene expression. This review summarized current state MSC-EV-based therapies highlighting potential clinical applications. We discussed challenges limitations translating these practice, inconsistent EV production, complex cargo composition, need targeted delivery strategies. Future research should focus on optimizing production characterization, identifying miRNAs, developing innovative systems maximize SCI.
Language: Английский
Citations
1
Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(3), P. 683 - 683
Published: Jan. 21, 2025
This narrative review focuses on innovative treatment approaches to diabetic retinopathy meet the urgent demand for advancements in managing both early and late stages of disease. Recent studies highlight potential adipose stem cells their secreted factors mitigating retinal complications diabetes, with promising results improving visual acuity reducing inflammation angiogenesis retinopathy. However, caution is warranted regarding safety long-term therapeutic effects transplantation. Bone marrow mesenchymal can also mitigate damage Studies demonstrate that bone cells-derived exosomes suppress Wnt/β-catenin pathway, oxidative stress, inflammation, retina, offering promise future treatments. Nanotechnology has ability precisely target retina minimize systemic side effects. Nanoparticles nanocarriers offer improved bioavailability, sustained release therapeutics, synergistic They be a new way effective prevention Activation modulation PPARα as means been widely investigated recent years demonstrated clinical trials. activation turned out method treating dyslipidemia, insulin sensitivity. The combination modulators small molecules offers an interesting perspective diseases’ therapy.
Language: Английский
Citations
1
Experimental Neurology, Journal Year: 2023, Volume and Issue: 363, P. 114374 - 114374
Published: March 11, 2023
Language: Английский
Citations
18
Ageing Research Reviews, Journal Year: 2024, Volume and Issue: 97, P. 102308 - 102308
Published: April 12, 2024
Language: Английский
Citations
7
Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15
Published: June 27, 2024
Exosomes, as a class of small extracellular vesicles closely related to the biological behavior various types tumors, are currently attracting research attention in cancer diagnosis and treatment. Regarding diagnosis, stability their membrane structure wide distribution body fluids render exosomes promising biomarkers. It is expected that exosome-based liquid biopsy will become an important tool for tumor future. For treatment, exosomes, “golden communicators” between cells, can be designed deliver different drugs, aiming achieve low-toxicity low-immunogenicity targeted delivery. Signaling pathways exosome contents also used safer more effective immunotherapy against tumors. Exosomes derived from range sources, exhibit characteristics well clinical application advantages therapies. In this review, we analyzed main sources have great potential broad prospects therapy. Moreover, compared therapeutic advantages, providing new ideas exosomes.
Language: Английский
Citations
6
ACS Nano, Journal Year: 2024, Volume and Issue: 18(34), P. 22793 - 22828
Published: Aug. 14, 2024
Extracellular vesicles (EVs) are nanoscale membrane of various sizes that can be secreted by most cells. EVs contain a diverse array cargo, including RNAs, lipids, proteins, and other molecules with functions intercellular communication, immune modulation, regulation physiological pathological processes. The biofluids in the eye, tears, aqueous humor, vitreous important sources for EV-based diagnosis ocular disease. Because molecular cargos may reflect biology their parental cells, these biofluids, as well blood, have been recognized promising candidates biomarkers early Moreover, also used therapeutics targeted drug delivery nanocarriers many disorders because low immunogenicity superior biocompatibility nature. In this review, we provide an overview recent advances field studies on We summarized origins applied disease, assessed different methods EV isolation from biofluid samples, highlighted bioengineering strategies systems, introduced latest applications treatment presented potential current clinical trials. Finally, briefly discussed challenges disease some issues concern better focusing translational future.
Language: Английский
Citations
6
Cells, Journal Year: 2022, Volume and Issue: 11(23), P. 3892 - 3892
Published: Dec. 2, 2022
Severe corneal damage leads to complete vision loss, thereby affecting life quality and impinging heavily on the healthcare system. Current clinical approaches manage wounds suffer from severe drawbacks, thus requiring development of alternative strategies. Of late, mesenchymal stromal/stem cell (MSC)-derived extracellular vesicles (EVs) have become a promising tool in ophthalmic field. In present study, we topically delivered bone-marrow-derived MSC-EVs (BMSC-EVs), embedded methylcellulose, murine model alkali-burn-induced order evaluate their role repair through histological molecular analyses, with support magnetic resonance imaging. Our data show that BMSC-EVs, used for first time this specific formulation damaged cornea, modulate death, inflammation angiogenetic programs injured tissue, leading faster recovery damage. These results were confirmed cadaveric donor-derived human epithelial cells vitro. Thus, BMSC-EVs dynamics are as new cell-free approach intervening burn wounds, especially avascularized region eye.
Language: Английский
Citations
28
Stem Cells International, Journal Year: 2023, Volume and Issue: 2023, P. 1 - 20
Published: Sept. 27, 2023
The most prevalent type of alopecia is androgenetic (AGA), which has a high prevalence but no effective treatment. Elevated dihydrotestosterone (DHT) level in the balding area was usually thought to be critical pathophysiology AGA. canonical Wnt/β-catenin signaling pathway plays key role promoting hair follicle development and sustaining cycle. Adipose-derived stem cell exosomes (ADSC-Exos) are widely used field regenerative medicine due advantages being free immune privileged. Still, few studies have reported therapeutic effect on disorders. As result, we sought understand how ADSC-Exos affected growth explore possibility that could counteract hair-growth-inhibiting effects DHT. This research using human organs, vitro dermal papilla cells, vivo animal models showed not only encouraged healthy also counteracted inhibitory DHT growth. Additionally, discovered increased Ser9 phosphorylated glycogen synthase kinase-3β levels facilitated nuclear translocation β-catenin, may been blocked by specific inhibitor dickkopf-related protein 1. Our findings suggested essential for regeneration, anticipated open up new possibilities clinical alopecia, particularly treatment
Language: Английский
Citations
14
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(3), P. 1774 - 1774
Published: Feb. 1, 2024
The blood-retinal barrier (BRB) is strongly compromised in diabetic retinopathy (DR) due to the detachment of pericytes (PCs) from retinal microvessels, resulting increased permeability and impairment BRB. Western blots, immunofluorescence ELISA were performed on adipose mesenchymal stem cells (ASCs) pericyte-like (P)-ASCs by co-cultured human endothelial (HRECs) under hyperglycemic conditions (HG), as a model DR. Our results demonstrated that: (a) platelet-derived growth factor receptor (PDGFR) its activated form more highly expressed monocultured P-ASCs than ASCs, this expression when with HRECs high glucose (HG); (b) transcription Nrf2 was cytoplasmic fraction ASCs P-ASC nuclear fraction, normal and, even more, HG conditions; (c) cytosolic phospholipase A2 activity prostaglandin E2 release, stimulated HG, significantly reduced HRECs; (d) HO-1 protein content higher HG-P-ASCs/HRECs P-ASCs/HRECs; (e) VEGF-A levels media HG-co-cultures P-ASCs/HRECs respect ASCs/HRECs. data obtained highlighted potential autologous differentiated future clinical applications based cell therapy counteract damage induced
Language: Английский
Citations
5