EFFECTS OF BIOPHYSICAL STIMULI ON OSTEOGENIC DIFFERENTIATION OF MESENCHYMAL STEM CELLS DOI

Miguel Angel Moncayo-Donoso,

Diego Alexander Garzón–Alvarado, Rick Visser

et al.

Biomedical Engineering Applications Basis and Communications, Journal Year: 2024, Volume and Issue: 36(05)

Published: June 28, 2024

Mesenchymal stem cells (MSCs) differentiate to that are part of tissues, such as bone and cartilage, both during ontogeny for the repair/regeneration renewal tissues postnatal life. Accordingly, MSCs can be a powerful tool regenerative medicine tissue engineering interventions in musculoskeletal deficiencies. found marrow connective exposed biochemical biophysical factors modulate their biological behavior. Given have been widely studied, research on effect stimuli differentiation is scarce. An overview impact osteogenic could shed light some signals consider when developing new cell therapies or products treatment traumatic defects diseases. The aim this review provide an differentiation. It focuses reviewing what has published compression, tension, hydrostatic pressure, shear stress, electromagnetic fields, low-intensity ultrasound, niche signaling – including topography substrate stiffness, paracrine juxtracrine regulation knowledge gained from studying how respond mechanical forces potential drive creation more advanced treatments conditions where bones injured. These rely either physical methods combination chemical approaches, offering possibilities improving health.

Language: Английский

Mesenchymal Stem Cell Culture within Perfusion Bioreactors Incorporating 3D‐Printed Scaffolds Enables Improved Extracellular Vesicle Yield with Preserved Bioactivity DOI Creative Commons
Stephanie M. Kronstadt,

Divya B. Patel,

Louis J. Born

et al.

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 12(20)

Published: March 17, 2023

Abstract Extracellular vesicles (EVs) are implicated as promising therapeutics and drug delivery vehicles in various diseases. However, successful clinical translation will depend on the development of scalable biomanufacturing approaches, especially due to documented low levels intrinsic EV‐associated cargo that may necessitate repeated doses achieve benefit certain applications. Thus, here effects a 3D‐printed scaffold‐perfusion bioreactor system assessed production bioactivity EVs secreted from bone marrow‐derived mesenchymal stem cells (MSCs), cell type widely generating with therapeutic potential. The results indicate perfusion culture induces an ≈40‐80‐fold increase (depending measurement method) MSC EV compared conventional culture. Additionally, generated using significantly improve wound healing diabetic mouse model, increased CD31 + staining bed tissue animals treated flask culture‐generated EVs. Overall, this study establishes solution major translational bottleneck, capacity for tunability specific applications general improvement alongside advancements 3D‐printing technologies.

Language: Английский

Citations

27
Recent advances in biomaterials for tissue-engineered constructs: Essential factors and engineering techniques DOI Creative Commons

Shiva Norouzi,

Nikoo Saveh Shemshaki,

Ehsan Norouzi

et al.

Materials Today Chemistry, Journal Year: 2024, Volume and Issue: 37, P. 102016 - 102016

Published: April 1, 2024

Tissue-engineered constructs can replicate the structural and physiological properties of natural tissues. The be designed to address transplantation issues affected by shortage donor tissues organs. One major concerns in tissue engineering is design development structures that improve interaction between materials cells provide an ideal platform for form functional tissue. Several contributing factors need considered fabricate constructs, including biomaterials, biological, topographical, biophysical, morphological either alone or combination. Here, we review application, advancement, future directions these essential designing developing regeneration. In particular, focus on original approaches tools construct parameters engineering.

Language: Английский

Citations

14
The corrosion and biological behavior of 3D-printed polycaprolactone/chitosan scaffolds as protective coating for Mg alloy implants DOI
Negin Khoshnood, John P. Frampton, S. R. Alavi Zaree

et al.

Surface and Coatings Technology, Journal Year: 2024, Volume and Issue: 477, P. 130368 - 130368

Published: Jan. 5, 2024

Language: Английский

Citations

11
Estrogen and estrogen receptors mediate the mechanobiology of bone disease and repair DOI
Vivian Y. Shi, Elise F. Morgan

Bone, Journal Year: 2024, Volume and Issue: 188, P. 117220 - 117220

Published: Aug. 5, 2024

Language: Английский

Citations

11
Therapeutic angiogenesis and tissue revascularization in ischemic vascular disease DOI Creative Commons
Xinyue Chen,

Wenlu Yu,

Jing Zhang

et al.

Journal of Biological Engineering, Journal Year: 2023, Volume and Issue: 17(1)

Published: Feb. 16, 2023

Ischemic vascular disease is a major healthcare problem. The keys to treatment lie in regeneration and restoration of perfusion. However, current treatments cannot satisfy the need for restore blood circulation. As biomedical research has evolved rapidly, variety potential alternative therapeutics been explored widely, such as growth factor-based therapy, cell-based material-based therapy including nanomedicine biomaterials. This review will comprehensively describe main pathogenesis injury ischemic disease, therapeutic function above three strategies, corresponding challenges, future directions.

Language: Английский

Citations

22
The bone marrow side of axial spondyloarthritis DOI
Daniele Mauro, Saviana Gandolfo,

Enrico Tirri

et al.

Nature Reviews Rheumatology, Journal Year: 2023, Volume and Issue: 19(8), P. 519 - 532

Published: July 5, 2023

Language: Английский

Citations

19
Functional enhancement strategies to potentiate the therapeutic properties of mesenchymal stromal cells for respiratory diseases DOI Creative Commons
Miquéias Lopes‐Pacheco, Patrícia R. M. Rocco

Frontiers in Pharmacology, Journal Year: 2023, Volume and Issue: 14

Published: March 16, 2023

Respiratory diseases remain a major health concern worldwide because they subject patients to considerable financial and psychosocial burdens result in high rate of morbidity mortality. Although significant progress has been made understanding the underlying pathologic mechanisms severe respiratory diseases, most therapies are supportive, aiming mitigate symptoms slow down their progressive course but cannot improve lung function or reverse tissue remodeling. Mesenchymal stromal cells (MSCs) at forefront regenerative medicine field due unique biomedical potential promoting immunomodulation, anti-inflammatory, anti-apoptotic antimicrobial activities, repair various experimental models. However, despite several years preclinical research on MSCs, therapeutic outcomes have fallen far short early-stage clinical trials for diseases. This limited efficacy associated with factors, such as reduced MSC homing, survival, infusion late disease. Accordingly, genetic engineering preconditioning methods emerged functional enhancement strategies potentiate actions MSCs thus achieve better outcomes. narrative review describes that investigated setting functionally properties These include changes culture conditions, exposure inflammatory environments, pharmacological agents other substances, manipulation enhanced sustained expression genes interest. Future directions challenges efficiently translating into practice discussed.

Language: Английский

Citations

12
Recent advances in mesenchymal stem/stromal cells (MSCs)‐based approaches for osteoarthritis (OA) therapy DOI
Ali Hassanzadeh, Nasim Vousooghi, Ramin Rahimnia

et al.

Cell Biology International, Journal Year: 2023, Volume and Issue: 47(6), P. 1033 - 1048

Published: March 30, 2023

Abstract Recently, mesenchymal stem/stromal cells (MSCs) transplantation has been introduced as a promising option to support cartilage structure and improve its function in preclinical models patients suffering from osteoarthritis (OA). MSCs strongly provoke their preferred influence vivo by inhibiting the inflammatory responses applying immunomodulation releasing anti‐inflammatory mediators such transforming growth factor‐β interleukin‐10. Such downregulate fibroblast‐like synoviocytes migration, leading chondroprotection. Furthermore, improving chondrocyte proliferation extracellular matrix hemostasis addition suppression of metalloproteinases activities can tissue organization. In this light, various published results have demonstrated that therapy considerably decrease pain restore knee OA patients. current review, we concentrated on recent advances MSCs‐based therapeutics elicit both chondrogenic chondroprotective impacts patients, focusing last decade results.

Language: Английский

Citations

11
Oscillatory fluid flow enhanced mineralization of human dental pulp cells DOI Creative Commons

Witsanu Yortchan,

Sasima Puwanun

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: Jan. 15, 2025

The purpose of this study is to evaluate the optimum frequency oscillatory fluid flow (OFF) for increasing osteogenesis in human dental pulp cells (DPCs) an incubating rocking shaker. DPCs from 3 donors were cultured osteogenic induction medium (OIM) and mechanical stimulation was applied using shaker at frequencies 0 (control), 10, 20, 30, 40 round per minute (RPM) 1 h/day, 5 days/week. Cell proliferation measured total protein quantification, activity by alkaline phosphatase (ALP) activity, calcium deposition, collagen production on days 7, 14, 21 culture. Results morphology 30 RPM group more clustered formed interconnections between cells. DPC showed no significant differences experiment groups. ALP day 7 deposition significantly higher than control Thus likely effective deposition. This uses strategies Tissue Engineering followed research topic about application stimulate oral maxillofacial hard tissue regeneration. In future, mineralization could be enhanced lab create a cell sheet. mineralized sheet then implanted into patient bone repair orofacial defects.

Language: Английский

Citations

0
3D Mechanical Response Stem Cell Complex Repairs Spinal Cord Injury by Promoting Neurogenesis and Regulating Tissue Homeostasis DOI Open Access
Jingwei Jiu, Haifeng Liu,

Dijun Li

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 24, 2025

Abstract Spinal cord injury (SCI) leads to acute tissue damage that disrupts the microenvironmental homeostasis of spinal cord, inhibiting cell survival and function, thereby undermining treatment efficacy. Traditional stem therapies have limited success in SCI, due difficulties maintaining inducing sustained differentiation into neural lineages. A new solution may arise from controlling fate cells by creating an appropriate mechanical microenvironment. In this study, response complex (MRSCC) is created as innovative therapeutic strategy for utilizing 3D bioprinting technology gelatin microcarriers (GM) loaded with mesenchymal (MSCs). GM creates optimal microenvironment MSCs growth paracrine activity. Meanwhile, allows accurate control spatial pore architecture characteristics construct encourage neuroregeneration. The MRSCC found activate Piezo1 channel prevent excessive nuclear translocation YAP, increasing neural‐related gene expression MSCs. Transplanting rats injuries boosts sensory motor recovery, reduces inflammation, stimulates regeneration neurons glial cells. offers a engineering can promote repair.

Language: Английский

Citations

0