Improving HPV-related health literacy in the Austrian population – A participatory research approach

Human Vaccines & Immunotherapeutics, Journal Year: 2025, Volume and Issue: 21(1)

Published: Feb. 25, 2025

Human papilloma virus (HPV) infections are widespread and affect a main part of the population. Long-term risks such include development HPV-induced cancers. Although available, preventive measures not utilized by Austrian population to their fullest extent. Only 40% all Austrians have completed vaccination programme only 46% aware HPV. This may be attributed lack awareness or an ineffective approach in disseminating information raise about this health issue. Our aim was determine which knowledge on HPV vaccinations is present identify preferred mode communication for receiving health-related issues, including objective implemented context participatory research project together with citizen scientists, whereby target group-oriented survey developed. Data from 889 participants, analyzed descriptive statistics, demonstrated significant gender- age-related differences information-seeking behavior. Men individuals under 18 were significantly less informed, especially regarding transmission routes HPV-related diseases. Women more vaccine's benefits higher rates. The use clear, concise content personal stories can foster engagement address misperceptions. Customized campaigns integration education into school curricula should facilitate early acquisition uptake.

Language: Английский

---

New insights into tumor microenvironment and HPV integrations in cervical cancer pathogenesis revealed by single-cell transcriptome data

Human Molecular Genetics, Journal Year: 2025, Volume and Issue: 34(11), P. 920 - 933

Published: March 27, 2025

HPV infection is common among women and can result in serious illnesses. This research utilizes single-cell RNA-sequencing (scRNA-seq) to study the connection between cellular heterogeneity integrations cervical histopathology. scRNA-seq was used examine normal patients those three disease stages: high-grade squamous intraepithelial lesions (HSIL), microinvasive carcinoma (MIC), epithelium cancer (CSCC) tissues. A method developed identify integration events from data. Our results indicated an increase epithelial cells a decrease columnar as progressed CSCC. We discovered genes that were differentially expressed across stages. Notably, more at level. The ratio of HPV-integrated increased tissue CSCC, eventually stabilizing. Several genes, such EGR1, S100A11, S100A8, KRT5, RPL34, ATP1B1, RPS4X EEF2, frequently integrated by patients. In contrast, like PAN3, BABAM2, SPEN, TCIM-SIRLNT, TEX41-PABPC1P2 KCNV1-LINC01608 showed frequent cells. RPS4X, PAN3 SPEN novel recurrent we observed patient or cell level this study. Additionally, found various types exhibited preferences samples provides valuable insight into mechanism HPV-induced standpoint, highlighting its clinical relevance.

Language: Английский

---

Human papillomavirus E2 proteins suppress innate antiviral signaling pathways

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 8, 2025

Human papillomavirus (HPV) is a major cause of cancers and benign lesions. High-risk (HR) types, including HPV16 HPV18, are strongly implicated in cervical other malignancies, while low-risk (LR) such as HPV11, predominantly associated with conditions. Although the immune evasion HPV oncoproteins E6 E7 extensively studied, immunomodulatory functions E2 protein remain poorly underexplored. This study elucidates role HPV11 proteins modulating innate responses, focusing on their interaction key antiviral signaling pathways. We demonstrate that effectively suppress activation pivotal pathways, RIG-I/MDA5-MAVS, TLR3-TRIF, cGAS-STING, JAK-STAT. Mechanistic analyses reveal interact core components type I interferon (IFN)-inducing inhibiting IRF3 phosphorylation nuclear translocation, thereby attenuating IFN expression. Additionally, disrupts JAK-STAT cascade by preventing assembly ISGF3 complex, comprising STAT1, STAT2, IRF9, ultimately transcription interferon-stimulated genes (ISGs). These findings underscore broader immunosuppressive proteins, complementing well-established mechanisms mediated E7. work advances our understanding HPV-mediated positions promising target for therapeutic strategies aimed at augmenting immunity HPV-associated diseases.

Language: Английский

---

The splicing factor kinase, SR protein kinase 1 (SRPK1) is essential for late events in the human papillomavirus life cycle

PLoS Pathogens, Journal Year: 2025, Volume and Issue: 21(4), P. e1012697 - e1012697

Published: April 9, 2025

Human papillomaviruses (HPV) infect epithelia to cause benign lesions or warts. However, the so-called “high risk” HPVs infecting anogenital region and oropharynx can precancerous that may progress malignant tumours. Understanding HPV life cycle is important discovery of novel antiviral therapies. uses cellular splicing produce full suite viral mRNAs. Members serine/arginine rich (SR) protein family positively regulate splicing. SR activity location regulated by phosphorylation their serine-arginine domains. kinases (SRPK) phosphorylate proteins. This licenses nuclear entry promotes together with another kinase, Clk1. SRPIN340 a specific inhibitor SRPK1. It has been reported inhibit replication HCV, Sindbis virus HIV. We show here inhibits expression transcription factor, E2. Loss E4 L1 late proteins was also observed. RNA sequencing showed treatment resulted in gene changes opposite those induced HPV16 infection. In particular, loss epithelial barrier restored. led alternative 935 RNAs pathway analysis predominance encoding involved chromatin conformation, DNA repair processing. Short term (two three days) not associated proliferation differentiation keratinocytes. Since SRPK1 controls E2 targeting this events it mediates, could be considered as therapeutic strategy for

Language: Английский

---

Human papilloma virus (HPV) mediated cancers: an insightful update

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 29, 2025

Language: Английский

---