Unlocking the genetic and biotechnological potential of Gloriosa superba to enhance its alkaloid production

Industrial Crops and Products, Journal Year: 2024, Volume and Issue: 211, P. 118144 - 118144

Published: Feb. 10, 2024

Language: Английский

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Physiological, anatomical and quality indexes of root tuber formation and development in chayote (Sechium edule)

BMC Plant Biology, Journal Year: 2023, Volume and Issue: 23(1)

Published: Sept. 7, 2023

Abstract Background Chayote is an underutilized species of Cucurbitaceae. It rich in nutrients such as protein, minerals, phenols and its extracts have anti-cardiovascular anti-cancer effects, making it a versatile plant for both medicinal culinary purposes. Although research on root tuber limited, they are starch structure similar to that potatoes, cassava, sweet potatoes. Therefore, can serve potential substitutes potatoes offer promising prospects agricultural industrial resources. However, the physiological cellular mechanisms chayote formation development still unclear. Results In this study, we observed growth habit ‘Tuershao’ (high yield tuber). The results revealed enlargement period lasts approximately 120 days, with early phase occurring during 0–30 rapid 30–90 maturation 90–120 days. Physiological indicators demonstrated gradual increase content developed. activities sucrose synthase (SUS) invertase (VIN) showed consistent trend, reaching highest level expansion period, which was key enzyme affecting expansion. Moreover, special petal like formed by secondary phloem xylem resulted enlargement, facilitating accumulation abundant within thin-walled cells structure. Principal component analysis further confirmed content, SUS VIN activities, well concentrations calcium (Ca), iron (Fe), selenium (Se), were major factors influencing development. low temperature environment not only promoted tubers but also enhanced nutritional substances. Conclusions These findings contribute deeper understanding developmental tubers, providing valuable guidance cultivation practices aimed at improving crop yield.

Language: Английский

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Combining Metabolomics and Transcriptomics to Reveal the Regulatory Mechanism of Taproot Enlargement in Panax ginseng

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(6), P. 5590 - 5590

Published: March 15, 2023

Ginseng is regarded as the “king of herbs” in China, with its roots and rhizomes used medicine, it has a high medicinal value. In order to meet market demand, artificial cultivation ginseng emerged, but different growth environments significantly affect root morphology garden ginseng. this study, we cultivated deforested land (CF-CG) farmland (F-CG) experimental materials. These two phenotypes were explored at transcriptomic metabolomic levels so understand regulatory mechanism taproot enlargement The results show that, compared those F-CG, thickness main CF-CG was increased by 70.5%, fresh weight taproots 305.4%. Sucrose, fructose ginsenoside accumulated CF-CG. During CF-CG, genes related starch sucrose metabolism up-regulated, while lignin biosynthesis down-regulated. Auxin, gibberellin abscisic acid synergistically regulated addition, sugar signaling molecule, T6P might act on auxin synthesis gene ALDH2 promote and, thus, participate development roots. summary, our study conducive clarifying molecular regulation ginseng, provides new insights for further exploration morphogenesis

Language: Английский

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Identification of BBX gene family and its function in the regulation of microtuber formation in yam

BMC Genomics, Journal Year: 2023, Volume and Issue: 24(1)

Published: June 26, 2023

BBX proteins play important roles in all of the major light-regulated developmental processes. However, no systematic analysis gene family regarding regulation photoperiodic microtuber formation has been previously performed yam. In this study, a on was conducted three yam species, with results, indicating that plays role regulating formation. These analyses included identification their evolutionary relationships, conserved domains, motifs, structure, cis-acting elements, and expressional patterns. Based these analyses, DoBBX2/DoCOL5 DoBBX8/DoCOL8 showing most opposite pattern expression during were selected as candidate genes for further investigation. Gene showed highest expressed leaves exhibited photoperiod responsive Besides, overexpression potato accelerated tuber under short-day (SD) conditions, whereas only enhanced accelerating effect dark conditions induction. Tuber number increased overexpressing plants dark, well SD. Overall, data generated study may form basis future functional characterizations yam, especially via response pathway.

Language: Английский

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Wind disturbance-based tomato seedlings growth control

Biosystems Engineering, Journal Year: 2024, Volume and Issue: 243, P. 82 - 92

Published: May 20, 2024

Language: Английский

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Characterization and expression analysis of SnRK2, PYL, and ABF/ AREB/ ABI5 gene families in sweet potato

PLoS ONE, Journal Year: 2023, Volume and Issue: 18(11), P. e0288481 - e0288481

Published: Nov. 3, 2023

Abscisic acid (ABA) signaling in plants is essential to several aspects of plant development, such as tolerance environmental stresses and growth. ABA also important for storage organ formation crops, sweet potato. However, the repertoire I. batatas gene families has not yet been fully characterized, so that it unclear which members these are necessary tuberization. Therefore, genome-wide identification potato ABF/ AREB/ ABI5, SnRK2, PYL was performed, along with phylogenetic, motif, cis-regulatory element (CRE), expression analyses. Nine ABF, eight eleven family were identified, there high sequence conservation among proteins revealed by phylogenetic motif The promoter sequences genes had multiple CREs involved hormone responses stress responses. In silico qRT-PCR analyses expressed various tissues IbABF3, IbABF4, IbDPBF3, IbDPBF4, IbPYL4, IbSnRK2.1, IbSnRK2.2 significantly during root development. These results an reference can be used functional validation studies better understand how elicits at molecular level.

Language: Английский

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Responses of belowground bud bank density of geophytes to environmental perturbations: a meta-analysis

Journal of Plant Ecology, Journal Year: 2024, Volume and Issue: 17(3)

Published: April 23, 2024

Abstract A belowground bud bank is a collection of asexual propagules produced by the underground storage organs geophytes. Renewal through banks main reproductive strategy The density reflects potential renewal capacity geophyte communities. However, effects different perturbation regimes and habitats on geophytes are not comprehensively understood. Moreover, whether types respond differently to perturbations still unclear. For this meta-analysis, relevant papers environmental were systematically collected. cumulative effect size among was analyzed. Overall, small, which may result from opposite or fluctuating responses perturbations. Drought negatively affected density. Environmental decreased rhizome but increased tiller In wetlands, no significant found for other habitat types. general, highly resistant resilient. Changes in depend type perturbations, plants distributed banks.

Language: Английский

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Candidate gene discovery for the biofortification of wheat grains with essential micronutrients for global food security

Cereal Research Communications, Journal Year: 2024, Volume and Issue: 53(1), P. 181 - 191

Published: July 8, 2024

Language: Английский

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Transcriptomic profiling and discovery of key transcription factors involved in adventitious roots formation from root cuttings of mulberry

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: July 15, 2024

ARs plays a crucial role in plant morphogenesis and development. The limited inefficient rooting of scions poses significant challenge to the efficiency quality clonal propagation forest trees silvicultural practices. Building on previous research conducted by our team, we found that applying IBA at concentration 1000 mg/L significantly enhanced mulberry rooting. This study aims uncover molecular mechanisms underlying this effect analyzing RNA sequencing data from phloem before after treatment with over time intervals 10, 20, 30, 40 days. We identified 5226 DEGs, which were then classified into GO terms KEGG pathways, showing enrichment hormone signaling processes. Using WGCNA, eight co-expression modules, two correlated treatment. Additionally, 18 transcription factors potentially facilitate formation identified, an exploratory analysis cis-regulatory elements associated these was conducted. findings provide comprehensive understanding offer theoretical support for discovery utilization exceptional genetic resources within species.

Language: Английский

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The molecular coordination of tuberization: Current status and future directions

Current Opinion in Plant Biology, Journal Year: 2024, Volume and Issue: 82, P. 102655 - 102655

Published: Nov. 8, 2024

Language: Английский

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