Danubian Animal Genetic Resources,
Journal Year:
2023,
Volume and Issue:
8(1), P. 7 - 13
Published: Aug. 3, 2023
The
aim
of
the
preliminary
study
was
to
improve
success
cryopreservation
stallion
spermatozoa.
effect
different
equilibration
lengths
and
two
techniques
for
freezing
semen
evaluated,
based
on
motility
viability
parameters.
Fresh
diluted
with
BotuCrio
extender
divided
into
3
groups
equilibrated
0.5h,
2h,
4h.
Subsequently,
half
straws
each
group
were
cryopreserved
in
vapours
liquid
nitrogen
or
automatic
freezer.
Results
spermatozoa
progressive
showed
decreasing
trend
increased
time
equilibration.
Moreover,
a
significant
difference
(p
<
0.05)
percentage
producing
ROS
found
between
frozen
compared
by
freezer
same
time.
Nevertheless,
results
this
are
character.
Experiments
using
higher
number
individuals
need
be
tested
order
find
best
procedure
Slovak
national
horse
breeds.
Czech Journal of Animal Science,
Journal Year:
2024,
Volume and Issue:
69(11), P. 431 - 440
Published: Nov. 28, 2024
Applicability
of
cryopreserved
insemination
doses
is
dependent
on
the
quality
semen
after
thawing.
The
aim
study
was
to
check
options
new
markers
for
assessment
sperm
predict
their
fertilisation
ability.
In
five
bulls
Pinzgau
breed,
fertilising
ability
determined
using
in
vitro
fertilisation.
Bulls
were
divided
according
achieved
blastocyst
rate:
with
good
(NOB
–
38.6%,
KAZ
28.9%,
GAL
29.3%)
and
low
(LOH
19.4%,
NUS
22.1%).
addition
motility
morphology,
we
monitored
common
physiological
characteristics:
viability,
apoptosis,
acrosomal
status,
capacitation,
mitochondrial
activity
generation
reactive
oxygen
species
flow
cytometry
procedure.
Novel
fertility-related
biomarkers,
such
as
ubiquitination,
overexpression
MKRN1,
SPTRX-3
PAWP
proteins
or
histone
modification
(H3K4me2),
also
analysed
by
cytometry.
From
all
parameters,
more
proper
characteristics
impaired
proved
be
high
incidence
apoptotic
(YO-PRO,
Caspase
3/7)
higher
counts
morphologically
abnormal
spermatozoa.
Although
(IVF)
test
can
an
advantageous
method
evaluating
ability,
there
are
still
differences
between
vivo
processes,
which
must
considered
example,
when
capacitation
status.
Veterinary Sciences,
Journal Year:
2023,
Volume and Issue:
10(7), P. 446 - 446
Published: July 8, 2023
The
present
study
aimed
to
evaluate
the
effects
of
melatonin
(MLT),
glutathione
(GSH),
and
their
combination
on
ram
semen
quality
after
thawing.
During
eight
weekly
sessions,
from
three
Merino
rams
was
pooled,
diluted
with
an
egg-yolk-based
extender,
divided
into
four
groups:
control,
1
mM
MLT,
5
GSH,
MLT
+
GSH.
Diluted
evaluated
before
freezing
process.
supplementation
GSH
at
had
a
deleterious
effect
total
motility
progressive
(TPM),
curvilinear
velocity
(VCL),
straight-line
(VSL),
average-path
(VAP),
linearity
(LIN),
straightness
(STR)
increased
slow
spermatozoa
(%).
also
negative
TPM,
VSL,
STR
in
semen.
In
thawed
semen,
TPM
compared
control
group.
VSL
lower
group
than
Additionally,
both
antioxidants
attenuated
BCF.
These
results
indicate
that
impairs
or
does
not
improve
sperm
kinetic
parameters
either
They
suggest
combined
plays
protective
role
against
these
effects.
Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Jan. 20, 2023
Abstract
The
present
study
investigated
the
effect
of
Rosemary
essential
oil
oral
Supplementation
on
development
reproductive
organs,
including
morphometric
measures,
accessory
genital
gland,
and
semen
quality
in
Barki
rams.
Sixty
rams
were
randomly
divided
into
two
equal
groups;
control
(G1)
(G2),
treated
with
2
mg/Kg
body
weight
per
day
for
45
days
group.
Ultrasonographic
measurements
blood
sampling
performed
15,
30,
treatment,
samples
collected
twice
a
week
after
15
quantitative
qualitative
analysis.
results
showed
significant
increase
testicular
parameter
measures
epididymal
tail
group
than
However,
seminal
vesicle,
Cowper's,
prostate
glands
highest
treatment.
Although
there
was
ejaculate
volume
(ml),
individual
motility
(%),
sperm
cell
concentration
(x109/ml)
group;
no
improvement
(P
>
0.01)
alive
spermatozoa
(%).
Furthermore,
treatment
positive
testosterone
levels,
tail,
motility,
viability.
result
revealed
that
supplementation
might
improve
parameters,
glands,
quality.
Danubian Animal Genetic Resources,
Journal Year:
2023,
Volume and Issue:
8(2), P. 65 - 72
Published: Aug. 15, 2023
Zemplin
Rabbit
and
Liptov
Bold-Spotted
are
Slovak
national
breeds.
Our
aim
was
to
characterize
the
sperm
quality
of
these
two
breeds,
as
characteristics
important
for
artificial
insemination
cryopreservation
biodiversity
conservation
tools.
For
this
evaluation,
samples
sexually
mature
(ZR)
males
(n
=
6)
Rabbits
(LR)
4)
were
used.
According
progressive
motility
(PM)
data
(CASA),
divided
into
groups:
A
(>30%
PM)
B
(<30%
PM).
In
addition
PM
(ZR-A:
48.6±3.8%,
ZR-B:
16±3.2%,
LR-A:
35.38±2.6%,
LR-B:
4±2.2%),
total
(TM)
69.1±4.1%,
35.5±4.1%,
59.1±3%,
26.9±4.1%)
morphological
abnormalities
29.7±1.2%,
40±4%,
34.3±4.3%,
48.3±4.7%)
also
assessed
using
CASA
system.
The
proportion
dead/live,
apoptotic
oxidative
damaged
spermatozoa
(Spz)
by
flow
cytometry
fluorescent
dyes:
SYBR-14,
Sytox
Green,
Yo-Pro-1
CellROX
respectively.
results
correspond
values
morphometry.
Sperm
with
less
than
30%
does
not
show
proper
values,
while
higher
is
suitable
further
analysis
use.
Danubian Animal Genetic Resources,
Journal Year:
2023,
Volume and Issue:
8(1), P. 7 - 13
Published: Aug. 3, 2023
The
aim
of
the
preliminary
study
was
to
improve
success
cryopreservation
stallion
spermatozoa.
effect
different
equilibration
lengths
and
two
techniques
for
freezing
semen
evaluated,
based
on
motility
viability
parameters.
Fresh
diluted
with
BotuCrio
extender
divided
into
3
groups
equilibrated
0.5h,
2h,
4h.
Subsequently,
half
straws
each
group
were
cryopreserved
in
vapours
liquid
nitrogen
or
automatic
freezer.
Results
spermatozoa
progressive
showed
decreasing
trend
increased
time
equilibration.
Moreover,
a
significant
difference
(p
<
0.05)
percentage
producing
ROS
found
between
frozen
compared
by
freezer
same
time.
Nevertheless,
results
this
are
character.
Experiments
using
higher
number
individuals
need
be
tested
order
find
best
procedure
Slovak
national
horse
breeds.
