HSP27/IL-6 axis promotes OSCC chemoresistance of cisplatin, migration and invasion by orchestrating macrophages via a positive feedback loop DOI Creative Commons
Ying Qi, Juan Cao, Mingjing Jiang

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 21, 2024

Abstract The mechanisms of interaction and crosstalk between tumor cells tumor-associated macrophages (TAMs) have provided novel options for intervening in progression. However, the molecular TAMs underlying oral squamous cell carcinoma (OSCC) invasio, migration chemoresistance remain unclear. This study sought to specifically investigate role tumor-cell-derived paracrine heat shock protein 27 (HSP27) OSCC invasion, potential TAMs. In this study, bioinformatic analysis IHC results demonstrated that expression level HSP27 was higher tissues patients with advanced lymph node metastasis than early stage non-metastatic patients, its positively correlated levels multidrug resistance-associated proteins macrophage infiltration. vivo, Survival low-expressing xenograft model mice inferior controls. vitro, TAMs-CM significantly up-regulated two types including CAL27 SCC9 cells. cell-derived regulated through mode reduced apoptosis induced by chemotherapeutic drug cisplatin cells, thus promoting OSCC. promoted secretion cytokine IL-6 from TAMs, whereas TAMs-derived enhanced chemoresistance, invasion an autocrine activates β-catenin pathway during process, additionally stem properties manner. Tumor-cell-derived enhancing epithelial–mesenchymal transition (EMT) via binding toll-like receptor 4 (TLR4) on surface HSP27/TLR4 polarization M2-like phenotype Respectively, signaling while EMT HSP27. Collectively, migration, orchestrating M2 a positive feedback loop. TAM-derived these progressions HSP27/IL-6 Targeting may be effective treatment strategy it is expected control progression improve prognosis recurrence patients.

Language: Английский

Systematic and comprehensive insights into HIF-1 stabilization under normoxic conditions: implications for cellular adaptation and therapeutic strategies in cancer DOI Creative Commons
Jiayi Zhang, Mingxuan Yao, Siyu Xia

et al.

Cellular & Molecular Biology Letters, Journal Year: 2025, Volume and Issue: 30(1)

Published: Jan. 6, 2025

Abstract Hypoxia-inducible factors (HIFs) are essential transcription that orchestrate cellular responses to oxygen deprivation. HIF-1α, as an unstable subunit of HIF-1, is usually hydroxylated by prolyl hydroxylase domain enzymes under normoxic conditions, leading ubiquitination and proteasomal degradation, thereby keeping low levels. Instead hypoxia, sometimes even in normoxia, HIF-1α translocates into the nucleus, dimerizes with HIF-1β generate then activates genes involved adaptive such angiogenesis, metabolic reprogramming, survival, which presents new challenges insights its role processes. Thus, review delves mechanisms HIF-1 maintains stability normoxia including but not limited giving transcriptional, translational, well posttranslational regulation underscore pivotal adaptation malignancy. Moreover, extensively cancer cardiovascular diseases potentially serves a bridge between them. An overview HIF-1-related drugs approved or clinical trials summarized, highlighting their potential capacity for targeting toxicity related treatment. The provides comprehensive insight HIF-1’s regulatory mechanism paves way future research therapeutic development.

Language: Английский

Citations

4
Omics-Based Investigations of Breast Cancer DOI Creative Commons

Anca-Narcisa Neagu,

Danielle Whitham, Pathea Bruno

et al.

Molecules, Journal Year: 2023, Volume and Issue: 28(12), P. 4768 - 4768

Published: June 14, 2023

Breast cancer (BC) is characterized by an extensive genotypic and phenotypic heterogeneity. In-depth investigations into the molecular bases of BC phenotypes, carcinogenesis, progression, metastasis are necessary for accurate diagnoses, prognoses, therapy assessments in predictive, precision, personalized oncology. This review discusses both classic as well several novel omics fields that involved or should be used modern investigations, which may integrated a holistic term, onco-breastomics. Rapid recent advances profiling strategies analytical techniques based on high-throughput sequencing mass spectrometry (MS) development have generated large-scale multi-omics datasets, mainly emerging from three ”big omics”, central dogma biology: genomics, transcriptomics, proteomics. Metabolomics-based approaches also reflect dynamic response cells to genetic modifications. Interactomics promotes view research constructing characterizing protein–protein interaction (PPI) networks provide hypothesis pathophysiological processes progression subtyping. The emergence new omics- epiomics-based multidimensional opportunities gain insights heterogeneity its underlying mechanisms. main epiomics (epigenomics, epitranscriptomics, epiproteomics) focused epigenetic DNA changes, RNAs modifications, posttranslational modifications (PTMs) affecting protein functions in-depth understanding cell proliferation, migration, invasion. Novel fields, such epichaperomics epimetabolomics, could investigate interactome induced stressors PPI metabolites, drivers BC-causing phenotypes. Over last years, proteomics-derived omics, matrisomics, exosomics, secretomics, kinomics, phosphoproteomics, immunomics, provided valuable data deep dysregulated pathways their tumor microenvironment (TME) immune (TIMW). Most these datasets still assessed individually using distinct approches do not generate desired expected global-integrative knowledge with applications clinical diagnostics. However, hyphenated approaches, proteo-genomics, proteo-transcriptomics, phosphoproteomics-exosomics useful identification putative biomarkers therapeutic targets. To develop non-invasive diagnostic tests discover BC, omics-based allow significant blood/plasma-based omics. Salivaomics, urinomics, milkomics appear integrative high potential early diagnoses BC. Thus, analysis circulome considered frontier liquid biopsy. Omics-based modeling, classification subtype characterization. future single-cell analyses.

Language: Английский

Citations

36
Chaperones—A New Class of Potential Therapeutic Targets in Alzheimer’s Disease DOI Open Access
Joanna Batko, Katarzyna Antosz, Weronika Miśków

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(6), P. 3401 - 3401

Published: March 17, 2024

The review describes correlations between impaired functioning of chaperones and co-chaperones in Alzheimer's disease (AD) pathogenesis. study aims to highlight significant lines research this field. Chaperones like Hsp90 or Hsp70 are critical agents regulating cell homeostasis. Due some conditions, aging, their activity is damaged, resulting β-amyloid tau aggregation. This leads the development neurocognitive impairment. Dysregulation one causes condition. Disorders molecules PP5, Cdc37, CacyBP/SIPTRAP1, CHIP protein, FKBP52, STIP1 play a key role AD FKBP52 co-chaperones. protein co-chaperone that switches Hsp70/Hsp90 complexes, binds Hsp70. Recognition precise processes allows for invention effective treatment methods. Potential drugs may either reduce levels inhibit accumulation Some substances neuroprotect from Aβ toxicity. Further studies on required understand fundamental tenets topic more entirely improve prevention AD.

Language: Английский

Citations

11
Eugenol-loaded functionalized TiO₂-reinforced chitosan-guar gum-based films with photocatalytic sterilization for active food packaging application DOI
Sonali Mohanty, Subhankar Paul

Food Packaging and Shelf Life, Journal Year: 2025, Volume and Issue: 49, P. 101477 - 101477

Published: March 19, 2025

Language: Английский

Citations

1
Role of Immune Cells and Receptors in Cancer Treatment: An Immunotherapeutic Approach DOI Creative Commons
Anirban Goutam Mukherjee, Uddesh Ramesh Wanjari,

Arunraj Namachivayam

et al.

Vaccines, Journal Year: 2022, Volume and Issue: 10(9), P. 1493 - 1493

Published: Sept. 7, 2022

Cancer immunotherapy moderates the immune system’s ability to fight cancer. Due its extreme complexity, scientists are working put together all puzzle pieces get a clearer picture of system. Shreds available evidence show connection between cancer and Immune responses tumors lymphoid malignancies influenced by B cells, γδT NK dendritic cells (DCs). immunotherapy, which encompasses adoptive therapy, monoclonal antibodies (mAbs), checkpoint CART has revolutionized contemporary treatment. This article reviews recent developments in cell regulation immunotherapy. Various options treat many diseases, particularly cancer, due progress various immunotherapies, such as antibodies, recombinant proteins, vaccinations (both preventative curative), cellular cytokines.

Language: Английский

Citations

38
Heat Shock Proteins, a Double-Edged Sword: Significance in Cancer Progression, Chemotherapy Resistance and Novel Therapeutic Perspectives DOI Open Access
Dominika Kunachowicz, Magdalena Król-Kulikowska,

Wiktoria Raczycka

et al.

Cancers, Journal Year: 2024, Volume and Issue: 16(8), P. 1500 - 1500

Published: April 14, 2024

Heat shock proteins (Hsps) are involved in one of the adaptive mechanisms protecting cells against environmental and metabolic stress. Moreover, large role these carcinogenesis process, as well chemoresistance, was noticed. This review aims to draw attention possibilities using Hsps developing new cancer therapy methods, indicate directions for future research on this topic. In order discuss matter, a thorough latest scientific literature carried out, taking into account importance selected from Hsp family, including Hsp27, Hsp40, Hsp60, Hsp70, Hsp90 Hsp110. One more characteristic features all is that they play multifaceted progression, which makes them an obvious target modern anticancer therapy. Some researchers emphasize directly inhibiting action proteins. turn, others point their possible use design vaccines, would work by inducing immune response various types cancer. Due possibilities, it believed may contribute progress oncoimmunology, thus help development therapies, be characterized higher effectiveness lower toxicity patients.

Language: Английский

Citations

8
Hsp70 and Calcitonin Receptor Protein in Extracellular Vesicles from Glioblastoma Multiforme: Biomarkers with Putative Roles in Carcinogenesis and Potential for Differentiating Tumor Types DOI Open Access
Giusi Alberti, Christian M. Sánchez‐López, Antonio Marcilla

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(6), P. 3415 - 3415

Published: March 18, 2024

Glioblastoma multiforme (GBM) is a malignancy of bad prognosis, and advances in early detection treatment are needed. GBM heterogenous, with varieties differing within tumor patient between patients. Means needed to distinguish these GMB forms, so that specific strategies can be deployed for management. We study the participation chaperone system (CS) carcinogenesis. The CS dynamic, its members moving around body extracellular vesicles (EVs) interacting components other physiological systems health disease, including GBM. Here, we describe finding high amounts Hsp70 (HSPA1A) calcitonin receptor protein (CTR) EVs patients present standardized protocol collecting, purifying, characterizing carrying CTR plasma-derived from were obtained just before ablative surgery (T0) 7 days afterwards (T1); was highly elevated at T0 less T1, greatly increased reduced below normal values T1. Our results encourage further research assess as biomarkers differentiating forms determine their roles

Language: Английский

Citations

6
Autoantibodies as biomarkers for breast cancer diagnosis and prognosis DOI Creative Commons

Ruozhu Yang,

Yi Han, Wenjun Yi

et al.

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: Nov. 14, 2022

Breast cancer is the most common in women worldwide and a substantial public health problem. Screening for breast mainly relies on mammography, which leads to false positives missed diagnoses especially non-sensitive patients with small tumors dense breasts. The prognosis of classified by tumor, node, metastasis (TNM) staging, but this method does not consider molecular characteristics tumor. As product immune response tumor-associated antigens, autoantibodies can be detected peripheral blood used as noninvasive, presymptomatic, low-cost biomarkers. Therefore, provide possible supplementary screening classification. This article introduces methods detect research progress made recent years potential direction examination treatment cancer.

Language: Английский

Citations

28
Mechanisms of thermal, acid, desiccation and osmotic tolerance of Cronobacter spp. DOI

Yanfei Yan,

Mengyuan Cao,

Jiaqi Ma

et al.

Critical Reviews in Food Science and Nutrition, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 23

Published: Jan. 3, 2025

Cronobacter spp. exhibit remarkable resilience to extreme environmental stresses, including thermal, acidic, desiccation, and osmotic conditions, posing significant challenges food safety. Their thermotolerance relies on heat shock proteins (HSPs), genomic islands, enhanced DNA repair mechanisms, metabolic adjustments, ensuring survival under high-temperature conditions. Acid tolerance is achieved through internal pH regulation, acid efflux pumps, proteins, allowing in acidic matrices the gastrointestinal tract. Desiccation mediated by accumulation of protective osmolytes like trehalose, stabilizing membranes withstand dryness, especially dry products. Similarly, stress supported compatible solutes such as trehalose glycine betaine, along with adaptations balance pressures. These mechanisms highlight adaptability diverse environments. Moreover, exposure sublethal heat, osmotic, dry, may induce homologous or cross-resistance, complicating control strategies. Understanding these essential mitigate risks spp., powdered infant formula (PIF), ensure

Language: Английский

Citations

0
Inhibition of CCT5-mediated asparagine biosynthesis and anti-PD-L1 produce synergistic antitumor effects in colorectal cancer DOI Creative Commons
Yujie Zhang,

Weiyi Zhao,

Ling Wu

et al.

Acta Pharmaceutica Sinica B, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0