HSP27/IL-6 axis promotes OSCC chemoresistance of cisplatin, migration and invasion by orchestrating macrophages via a positive feedback loop DOI Creative Commons
Ying Qi, Juan Cao, Mingjing Jiang

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 21, 2024

Abstract The mechanisms of interaction and crosstalk between tumor cells tumor-associated macrophages (TAMs) have provided novel options for intervening in progression. However, the molecular TAMs underlying oral squamous cell carcinoma (OSCC) invasio, migration chemoresistance remain unclear. This study sought to specifically investigate role tumor-cell-derived paracrine heat shock protein 27 (HSP27) OSCC invasion, potential TAMs. In this study, bioinformatic analysis IHC results demonstrated that expression level HSP27 was higher tissues patients with advanced lymph node metastasis than early stage non-metastatic patients, its positively correlated levels multidrug resistance-associated proteins macrophage infiltration. vivo, Survival low-expressing xenograft model mice inferior controls. vitro, TAMs-CM significantly up-regulated two types including CAL27 SCC9 cells. cell-derived regulated through mode reduced apoptosis induced by chemotherapeutic drug cisplatin cells, thus promoting OSCC. promoted secretion cytokine IL-6 from TAMs, whereas TAMs-derived enhanced chemoresistance, invasion an autocrine activates β-catenin pathway during process, additionally stem properties manner. Tumor-cell-derived enhancing epithelial–mesenchymal transition (EMT) via binding toll-like receptor 4 (TLR4) on surface HSP27/TLR4 polarization M2-like phenotype Respectively, signaling while EMT HSP27. Collectively, migration, orchestrating M2 a positive feedback loop. TAM-derived these progressions HSP27/IL-6 Targeting may be effective treatment strategy it is expected control progression improve prognosis recurrence patients.

Language: Английский

Evaluating the expression of heat shock protein 27 and topoisomerase II α in a retrospective cohort of patients diagnosed with locally advanced breast cancer and treated with neoadjuvant anthracycline-based chemotherapies DOI Creative Commons

Yi-Xuan Zhuang,

Fan Zhang, Yue Xu

et al.

Frontiers in Oncology, Journal Year: 2023, Volume and Issue: 13

Published: Aug. 15, 2023

Neoadjuvant anthracycline-based chemotherapy (NAC) is a major regimen for the treatment of local advanced breast cancer (LABC), while resistance to NAC remains paramount clinical obstacle. To investigate role heat shock protein 27 (Hsp27) and/or topoisomerase IIα (TopoIIα) in LABC patients treated with NAC, we performed this retrospective study. Associations Hsp27 transcripts clinic-pathological characteristics, survival and drug response were investigated public databases. Hsp27-related genes identified, followed by functional enrichment analyses. Besides, two protein-protein interaction networks built. Then, tumors from 103 who diagnosed received collected, TopoIIα examined Immunohistochemistry (IHC). Chi-square or Fisher's exact tests performed, as well Either at transcriptional level databases tested IHC, high was associated aggressive tumor characteristics such lymph node invasion resistance. mostly involved metabolic pathway gamete generation biological process. An elevated indicated poor prognosis (log-rank test P = 0.002 0.004 disease-free [DFS] overall [OS], respectively), it might not be an independent predictor. Of note, expression (TopoIIα+) less likely express (Hsp27+), contrast those negativity (31.1% vs. 86.2%, P<0.001), analyses revealed that Hsp27+ TopoIIα- had significantly lower DFS OS < 0.001 0.001, other three groups. cancers more predictable when concomitantly considering expression.

Language: Английский

Citations

2
2-Aminoethyl Dihydrogen Phosphate (2-AEH2P) Associated with Cell Metabolism-Modulating Drugs Presents a Synergistic and Pro-Apoptotic Effect in an In Vitro Model of the Ascitic Ehrlich Tumor DOI Creative Commons
Monique Gonçalves Alves, Laertty Garcia de Sousa Cabral,

Paulo G. F. Totti

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(1), P. 109 - 109

Published: Jan. 4, 2024

The progression and maintenance of cancer characteristics are associated with cellular components linked to the tumor non-cellular pro-tumoral properties. Pharmacological association antagonists tumor, such as anti- pro-apoptotic drugs, represents a novel adjuvant strategy. In this study, antiproliferative, pro-apoptotic, pharmacological effects combination monophosphoester 2-AEH2P Simvastatin, Coenzyme Q10, chemotherapeutic drug paclitaxel, colony-stimulating factor (GM-CSF) were evaluated. Tests conducted determine cytotoxic activity using MTT method, cell cycle phases, fragmented DNA by flow cytometry, mitochondrial membrane potential, expression markers Bcl2, TNF-α/DR-4, Cytochrome c, caspase 3, P53, analysis profiles Synergy Finder 2.0 Software. results showed synergistic effect among combinations, compared individual treatments other drugs. addition, there was modulation marker expression, indicating immunomodulatory 2-AEH2P. revealed that cells treated GM-CSF + exhibited effect, while groups Simvastatin additive effects. Furthermore, treatment paclitaxel (12 h) resulted in significant reduction potential. combinations for normal did not exhibit deleterious mammary carcinomatosis (EAT) cells.

Language: Английский

Citations

0
Clinicopathologic significance of heat shock protein 60 as a survival predictor in breast carcinoma DOI Creative Commons
Qing Wang,

S. Chen,

Zhihong Wu

et al.

Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14

Published: Aug. 19, 2024

Background While Heat Shock Protein 60 (HSP60) has been linked to human tumor, its clinic significance specifically in breast carcinoma is unclear. This investigation aims retrospectively evaluate how HSP60 protein levels relate survival outcomes among patients diagnosed with carcinoma. Methods Evaluation of 206 and receiving treatment from January 2012 April 2018, carried out retrospectively. The level determined by immunohistochemical. Results study provided evidence a distinct upregulation expression tumor samples contrast adjacent normal tissue samples. Additionally, heightened was advanced T stage (P = 0.046), N 0.034), metastasis 0.016), pathological grading 0.012), adjuvant therapy utilization 0.004). Moreover, elevated proteins exhibited significant inverse correlation overall (OS) [hazard ratio (HR) 1.598, P 0.018] progression-free (PFS) (HR 1.600, 0.017) univariate analyses. results multivariate analyses highlighted may serve as an independent predictor for both OS PFS 1.525, 0.034; HR 1.528, 0.033, respectively). Conclusion involvement progression suggests potential clinical relevance target validation prognostic assessment the disease.

Language: Английский

Citations

0
Simultaneous targeting and suppression of heat shock protein 60 to overcome heat resistance and induce mitochondrial death of tumor cells in photothermal immunotherapy DOI Creative Commons

Yiling Meng,

Tao Wen, Xuanxin Liu

et al.

Materials Today Bio, Journal Year: 2024, Volume and Issue: 29, P. 101282 - 101282

Published: Sept. 30, 2024

Language: Английский

Citations

0
HSP27/IL-6 axis promotes OSCC chemoresistance of cisplatin, migration and invasion by orchestrating macrophages via a positive feedback loop DOI Creative Commons
Ying Qi, Juan Cao, Mingjing Jiang

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 21, 2024

Abstract The mechanisms of interaction and crosstalk between tumor cells tumor-associated macrophages (TAMs) have provided novel options for intervening in progression. However, the molecular TAMs underlying oral squamous cell carcinoma (OSCC) invasio, migration chemoresistance remain unclear. This study sought to specifically investigate role tumor-cell-derived paracrine heat shock protein 27 (HSP27) OSCC invasion, potential TAMs. In this study, bioinformatic analysis IHC results demonstrated that expression level HSP27 was higher tissues patients with advanced lymph node metastasis than early stage non-metastatic patients, its positively correlated levels multidrug resistance-associated proteins macrophage infiltration. vivo, Survival low-expressing xenograft model mice inferior controls. vitro, TAMs-CM significantly up-regulated two types including CAL27 SCC9 cells. cell-derived regulated through mode reduced apoptosis induced by chemotherapeutic drug cisplatin cells, thus promoting OSCC. promoted secretion cytokine IL-6 from TAMs, whereas TAMs-derived enhanced chemoresistance, invasion an autocrine activates β-catenin pathway during process, additionally stem properties manner. Tumor-cell-derived enhancing epithelial–mesenchymal transition (EMT) via binding toll-like receptor 4 (TLR4) on surface HSP27/TLR4 polarization M2-like phenotype Respectively, signaling while EMT HSP27. Collectively, migration, orchestrating M2 a positive feedback loop. TAM-derived these progressions HSP27/IL-6 Targeting may be effective treatment strategy it is expected control progression improve prognosis recurrence patients.

Language: Английский

Citations

0