Low shear stress protects chondrocytes from IL-1β-induced apoptosis by activating ERK5/KLF4 signaling and negatively regulating miR-143-3p

Journal of Orthopaedic Surgery and Research, Journal Year: 2024, Volume and Issue: 19(1)

Published: Oct. 15, 2024

This study investigated the protective effects of low fluid shear stress (FSS ≤ 2 dyn/cm²) against interleukin-1β (IL-1β)-induced chondrocyte apoptosis and explored underlying molecular mechanisms.

Language: Английский

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Epileptic seizures induced by pentylenetetrazole kindling accelerate Alzheimer-like neuropathology in 5×FAD mice

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 31, 2024

Clinical studies have shown that epileptic seizures worsen Alzheimer's disease (AD) pathology and related cognitive deficits; however, the underlying mechanism is unclear. To assess effects of on progression AD, chronic temporal lobe epilepsy was induced in five familial AD mutation (5×FAD) mice by kindling with chemoconvulsant pentylenetetrazole (PTZ) at 3-3.5 months age. The amyloidogenic pathway, tauopathy, synaptic damage, neuronal death, neurological inflammatory response associated kinase signaling pathway dysregulation were examined 9 We found APP, p-APP, BACE1, Aβ kinase-associated p-tau levels elevated after PTZ 5×FAD mice. In addition, exacerbated hippocampal damage cell as determined scanning electron microscopy terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) staining, respectively. Finally, neuroinflammation markers GFAP Iba1, well cytokine IL-1β, increased insult. profoundly extracellular regulated (ERK)-death-associated protein (DAPK) overactivation, acute ERK inhibitor treatment downregulated production p-APP long-term use antiseizure drug carbamazepine (CBZ) alleviated seizure-induced accelerated amyloid tau ERK-DAPK overactivation Collectively, these results demonstrate increases AD-like neuropathology are partially suggesting axis could be a new therapeutic target for patients comorbid seizures.

Language: Английский

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Bioinformatics and Experimental Identification of circ_0001535 Associated with Diagnosis and Development of Alzheimer's Disease

Journal of Integrative Neuroscience, Journal Year: 2023, Volume and Issue: 22(4)

Published: July 26, 2023

Background: Alzheimer’s disease (AD) is a type of frequently occurs in the elderly population. Diagnosis and treatment methods for this are still lacking, more research required. In addition, little known about function circular RNAs (circRNAs) AD. Methods: research, RNA expression data AD from Gene Expression Omnibus (GEO) database were downloaded. The levels circRNAs cerebrospinal fluid samples healthy participants patients measured by reverse transcription‑quantitative PCR (RT-qPCR). diagnosed value differential expressed was analyzed with Receiver operating characteristic curve (ROC). Pathways related to circ_0001535 found using gene set enrichment analysis (GSEA) Metascape. direct interactions between E2F transcription factor 1 (E2F1) or E2F1 dihydrofolate reductase (DHFR) verified Chromatin immunoprecipitation (ChIP) Binding Protein Immunoprecipitation (RIP) assays. Cell Counting Kit-8 (CCK8) flow cytometry used identify circ_0001535/E2F1/DHFR axis on proliferation apoptosis cells. Results: total, 12 have been linked diagnosis. 7 differed control groups. Circ_0001535 had most diagnose among these circRNAs. Hence, regarded as key circRNA present study. E2F1/DHFR predicted be regulated circ_0001535. IP assays experiment results showed that could bind promoter region DHFR vitro overexpression promote expression, while knock down inhibit SH-SY5Y Finally, rescue experiments suggested reduce Aβ25-35-induced cell facilitate through axis. Conclusions: Our can offer important information regarding role specific environment point future areas therapeutic intervention

Language: Английский

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Death-associated protein kinase 1 phosphorylates MDM2 and inhibits its protein stability and function

Archives of Pharmacal Research, Journal Year: 2023, Volume and Issue: 46(11-12), P. 882 - 896

Published: Oct. 7, 2023

Language: Английский

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Insights in RNA: 2022

Frontiers research topics, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

it is a pioneering approach to the world of academia, radically improving way scholarly research managed.The grand vision Frontiers where all people have an equal opportunity seek, share and generate knowledge.Frontiers provides immediate permanent online open access its publications, but this alone not enough realize our goals. journal seriesThe series multi-tier interdisciplinary set openaccess, journals, promising paradigm shift from current review, selection dissemination processes in academic publishing.All journals are driven by researchers for researchers; therefore, they constitute service community.At same time, operates on revolutionary invention, tiered publishing system, initially addressing specific communities scholars, gradually climbing up broader public understanding, thus serving interests lay society, too. Dedication qualityEach article landmark highest quality, thanks genuinely collaborative interactions between authors review editors, who include some world's best academicians.Research must be certified peers before entering stream knowledge that may eventually reach -and shape society; only applies most rigorous unbiased reviews.Frontiers revolutionizes freely delivering outstanding research, evaluated with no bias both social point view.By applying advanced information technologies, catapulting into new generation.

Language: Английский

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