Journal of Functional Biomaterials,
Journal Year:
2024,
Volume and Issue:
15(3), P. 67 - 67
Published: March 10, 2024
The
study
and
characterization
of
the
biophysical
properties
membranes
drug–membrane
interactions
represent
a
critical
step
in
drug
development,
as
biological
act
barrier
that
must
overcome
to
reach
its
active
site.
Liposomes
are
widely
used
delivery
circumvent
poor
aqueous
solubility
most
drugs,
improving
systemic
bioavailability
pharmacokinetics.
Further,
they
can
be
targeted
deliver
specific
disease
sites,
thus
decreasing
load,
reducing
side
effects
adherence
treatment.
To
improve
during
liposome
preparation,
DMSO
is
solvent.
This
raises
concern
about
potential
effect
on
leads
us
investigate,
using
DSC
EPR,
influence
behavior
lipid
model
DMPC
DPPC.
In
addition,
we
tested
interaction,
compounds
with
different
hydrophobicity
varying
content,
same
experimental
techniques.
Overall,
it
was
found
up
10%
DMSO,
changes
bilayer
fluidity
or
thermotropic
studied
liposomes
were
not
significant,
within
uncertainty.
For
higher
concentrations
there
stabilization
both
gel
rippled
phases,
increased
DPPC
leading
an
increase
membrane
permeability.
Polymers,
Journal Year:
2023,
Volume and Issue:
15(3), P. 782 - 782
Published: Feb. 3, 2023
Liposomes
carry
various
compounds
with
applications
in
pharmaceutical,
food,
and
cosmetic
fields,
the
administration
route
is
especially
parenteral,
oral,
or
transdermal.
are
used
to
preserve
release
internal
components,
thus
maintaining
properties
of
compounds,
stability
shelf
life
encapsulated
products,
their
functional
benefits.
The
main
problem
obtaining
liposomes
at
industrial
level
low
due
fragile
phospholipid
membranes.
To
increase
liposomes,
bilayers
have
been
modified
different
coating
materials
developed
studied,
both
for
pharmaceutical
field
food
field.
In
field,
need
no
additional
because
liposomal
formulation
intended
a
fast
penetration
into
skin.
aim
this
review
provide
current
knowledge
regarding
physical
chemical
factors
that
influence
stability,
fields
containing
sensitive
absorption
commercial
products
obtained
through
technologies
available
on
market.
npj Vaccines,
Journal Year:
2023,
Volume and Issue:
8(1)
Published: Oct. 9, 2023
Some
studies
have
shown
that
lyophilization
significantly
improves
the
stability
of
mRNA-LNPs
and
enables
long-term
storage
at
2-8
°C.
However,
there
is
little
research
on
process
mRNA-lipid
nanoparticles
(LNPs).
Most
previous
used
empirical
with
only
a
single
lyoprotectant,
resulting
in
low
efficiency,
often
requiring
40-100
h.
In
present
study,
an
efficient
method
suitable
for
was
designed
optimized,
shortening
total
length
to
8-18
h,
which
reduced
energy
consumption
production
costs.
When
mixed
lyoprotectant
composed
sucrose,
trehalose,
mannitol
added
mRNA-LNPs,
eutectic
point
collapse
temperature
system
were
increased.
The
lyophilized
product
had
ginger
root-shaped
rigid
structure
large
porosity,
tolerated
rapid
increases
efficiently
removed
water.
addition,
rapidly
rehydrated
good
particle
size
distribution,
encapsulation
rate,
mRNA
integrity.
stable
°C,
they
did
not
reduce
immunogenicity
vivo
or
vitro.
Molecular
dynamics
simulation
compare
phospholipid
molecular
layer
aqueous
anhydrous
environments
elucidate
mechanism
improve
mRNA-LNPs.
This
platform
accessibility
Pharmaceutics,
Journal Year:
2024,
Volume and Issue:
17(1), P. 36 - 36
Published: Dec. 30, 2024
Liposome-based
drug
delivery
technologies
have
showed
potential
in
enhancing
medication
safety
and
efficacy.
Innovative
loading
release
mechanisms
highlighted
this
review
of
next-generation
liposomal
formulations.
Due
to
poor
kinetics
capacity,
conventional
liposomes
limited
clinical
use.
Scientists
developed
new
carrier
control
encapsulation
methods
address
these
limits.
Drug
can
be
optimized
by
creating
lipid
compositions
that
match
a
drug's
charge
hydrophobicity.
By
selecting
lipids
adding
co-solvents
or
surfactants,
scientists
increased
formulations
while
maintaining
stability.
Nanotechnology
has
also
created
multifunctional
with
triggered
personalized
delivery.
Surface
modification
like
PEGylation
ligand
conjugation
direct
disease
regions,
improving
therapeutic
efficacy
reducing
off-target
effects.
In
addition
loading,
researchers
focused
on
spatiotemporal
modulation
release.
Stimuli-responsive
drugs
response
bodily
signals.
Liposomes
pH-
temperature-sensitive.
To
improve
reduce
systemic
toxicity,
added
stimuli-responsive
components
membranes
precisely
kinetics.
Advanced
magnetic
targeting
ultrasound.
Pro
Drug,
RNA
approach
may
administration.
Magnetic
helps
aggregate
at
illness
sites
improves
delivery,
whereas
ultrasound-mediated
facilitates
on-demand
encapsulated
medicines.
This
covers
recent
preclinical
research
showing
the
promise
for
cancer,
infectious
diseases,
neurological
disorders
inflammatory
disorders.
The
transfer
innovative
from
lab
practice
involves
key
difficulties
such
scalability,
manufacturing
difficulty,
regulatory
Applied Microbiology and Biotechnology,
Journal Year:
2024,
Volume and Issue:
108(1)
Published: June 5, 2024
Abstract
Lactobacillus
delbrueckii
subsp.
bulgaricus
and
Lactiplantibacillus
plantarum
are
two
lactic
acid
bacteria
(LAB)
widely
used
in
the
food
industry.
The
objective
of
this
work
was
to
assess
resistance
these
freeze-
spray-drying
study
mechanisms
involved
their
loss
activity.
culturability
acidifying
activity
were
measured
determine
specific
activity,
while
membrane
integrity
studied
by
flow
cytometry.
glass
transitions
temperature
water
dried
bacterial
suspensions
also
determined.
Fourier
transform
infrared
(FTIR)
micro-spectroscopy
biochemical
composition
cells
an
aqueous
environment.
All
experiments
performed
after
freezing,
drying
storage
at
4,
23
37
°C.
results
showed
that
Lb.
CFL1
sensitive
osmotic,
mechanical,
thermal
stresses,
Lpb.
WCFS1
tolerated
better
first
types
stress
but
more
stress.
Moreover,
FTIR
suggested
sensitivity
freeze-drying
could
be
attributed
cell
wall
degradation,
whereas
changes
nucleic
acids
proteins
would
responsible
heat
inactivation
both
strains
associated
with
spray-drying.
According
activation
energy
values
(47–85
kJ/mol),
functionality
during
is
a
chemically
limited
reaction.
Still,
physical
properties
glassy
matrix
played
fundamental
role
rates
transition
40
°C
above
needed
reach
good
preservation
storage.
Key
points
•
Specific
bands
proposed
as
markers
mechanic
all
three
only
Activation
revealed
reactions
ruled
Graphical
abstract
Expert Opinion on Drug Delivery,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 24, 2025
The
eye
is
a
complex
organ
with
several
anatomical
and
physiological
barriers
that
make
ocular
drug
delivery
an
ongoing
challenge.
Transfersomes
(TFS)
are
deformable
vesicles
have
extensively
been
applied
to
enhance
transdermal
delivery.
However,
their
application
in
remains
largely
unexplored.
This
review
highlights
the
challenges
typically
associated
emphasizes
inherent
properties
of
TFS
enable
them
overcome
these
challenges.
influence
excipients
critical
process
parameters
on
characteristics
discussed
detail
emphasis
fabrication
characterization
techniques
employed
for
development
optimization.
Furthermore,
recent
studies
evaluating
depth.
unique
stress-responsive
nature
makes
promising
carriers
further
research
this
direction
needed
understand
penetration
mechanism
elucidate
potential
sustained
targeted
tissues.
Moreover,
optimize
stability
scalability
encourage
translation
market.
The Journal of Physical Chemistry B,
Journal Year:
2024,
Volume and Issue:
128(20), P. 4922 - 4930
Published: May 11, 2024
The
disaccharide
trehalose
is
generally
acknowledged
as
a
superior
stabilizer
of
proteins
and
other
biomolecules
in
aqueous
environments.
Despite
many
theories
aiming
to
explain
this,
the
stabilization
mechanism
still
far
from
being
fully
understood.
This
study
compares
stabilizing
properties
with
those
structurally
similar
sucrose.
stability
has
been
evaluated
for
two
proteins,
lysozyme
myoglobin,
at
both
low
high
temperatures
by
determining
glass
transition
temperature,
Tg,
denaturation
Tden.
results
show
that
sucrose-containing
samples
exhibit
higher
Tden
than
corresponding
trehalose-containing
samples,
particularly
water
contents.
better
effect
sucrose
may
be
explained
fact
sucrose,
greater
extent,
binds
directly
protein
surface
compared
trehalose.
Both
sugars
elevation
an
increasing
sugar-to-protein
ratio,
which
allows
more
complete
sugar
shell
around
molecules.
Finally,
no
synergistic
effects
were
found
combining
Conclusively,
exact
vary
influenced
temperature-dependent
interactions
between
protein,
sugar,
water.
variability
can
make
under
some
conditions
others.
