Journal of Stem Cells and Regenerative Medicine,
Journal Year:
2024,
Volume and Issue:
unknown
Published: May 31, 2024
Osteoarthritis
(OA)
is
a
degenerative
disease
that
causes
chronic
pain
and
disability
worldwide.
This
mainly
caused
by
IL-1β
TNF-α,
which
lead
to
cartilage
degradation
inhibit
the
repair
capacity
of
damaged
cartilage.
Recent
studies
have
shown
amniotic
fluid
mesenchymal
stem
cells
(AF-MSCs)
secrete
proteins
can
effectively
help
in
treatment
OA.
However,
underlying
mechanism
still
unclear.
Therefore,
aim
this
study
was
investigate
effects
mechanisms
behind
healing
properties
AF-MSC
secretome
(AFS-se)
under
OA
conditions.
involved
growing
chondrocyte
progenitor
(CPCs)
traumatized
tissues
presence
cytokines
mimic
AFS-se
then
added
culture
medium
determine
its
effect
on
CPCs
Cell
migration,
endogenous
cell
outgrowth,
expression
chondrogenic
anabolic
genes,
NF-κB
MAPK
signaling
pathways
were
examined
study.
inhibited
inflammatory
TNF-α
significantly
reducing
ERK
phosphorylation
pathway
decreasing
downstream
proinflammatory
COX2
products.
The
impaired
recovered
their
ability
migrate,
injured
osteoarthritic
able
regrow
response
stimuli.
Additionally,
genes
such
as
Journal of Inflammation Research,
Journal Year:
2023,
Volume and Issue:
Volume 16, P. 2477 - 2489
Published: June 1, 2023
Abstract:
Secondary
chronic
neuropathic
pain
(NP)
in
addition
to
sensory,
motor,
or
autonomic
dysfunction
can
significantly
reduce
quality
of
life
after
spinal
cord
injury
(SCI).
The
mechanisms
SCI-related
NP
have
been
studied
clinical
trials
and
with
the
use
experimental
models.
However,
developing
new
treatment
strategies
for
SCI
patients,
poses
challenges.
inflammatory
response
following
promotes
development
NP.
Previous
studies
suggest
that
reducing
neuroinflammation
improve
NP-related
behaviors.
Intensive
roles
non-coding
RNAs
discovered
ncRNAs
bind
target
mRNA,
act
between
activated
glia,
neuronal
cells,
other
immunocytes,
regulate
gene
expression,
inhibit
inflammation,
influence
prognosis
Keywords:
non‐coding
RNAs,
neuroinflammation,
pain,
Frontiers in Pain Research,
Journal Year:
2023,
Volume and Issue:
4
Published: Nov. 13, 2023
Orofacial
pain
remains
a
significant
health
issue
in
the
United
States.
Pain
originating
from
orofacial
region
can
be
composed
of
complex
array
unique
target
tissue
that
contributes
to
varying
success
management.
Long-term
use
analgesic
drugs
includes
adverse
effects
such
as
physical
dependence,
gastrointestinal
bleeding,
and
incomplete
efficacy.
The
mesenchymal
stem
cells
for
their
relieving
properties
has
garnered
increased
attention.
In
addition
preclinical
clinical
results
showing
cell
analgesia
non-orofacial
pain,
studies
have
also
shown
promising
treatment.
Here
we
discuss
outcomes
treatment
compare
different
tissues
origin.
We
mechanism
underlying
these
analgesic/anti-nociceptive
properties,
including
role
immune
endogenous
opioid
system.
Lastly,
advancements
methods
procedures
treat
patients
experiencing
with
are
discussed.
Animals,
Journal Year:
2023,
Volume and Issue:
13(13), P. 2195 - 2195
Published: July 4, 2023
The
aim
of
the
study
was
to
investigate
potential
cell-based
regenerative
therapy
for
elbow
joints
affected
by
osteoarthritis.
Interest
focused
on
two
intra-articular
applications
amnion-derived
mesenchymal
stem
cells
(A-MSCs)
a
group
different
breeds
dogs
with
osteoarthritis
(13
joints).
Two
injections
were
performed
14
days
apart.
We
evaluated
synovial
fluid
biomarkers,
such
as
IFN-γ,
IL-6,
IL-15,
IL-10,
MCP-1,
TNF-α,
and
GM-CSF,
multiplex
fluorescent
micro-bead
immunoassay
in
treated
elbows
(n
=
13)
(day
0,
day
14,
28)
control
9).
Kinematic
gait
analysis
determined
joint
range
motion
(ROM)
before
after
each
A-MSCs
application.
28.
pointed
out
improvement
average
from
0
(38.45
±
5.74°),
(41.7
6.04°),
28
(44.78
4.69°)
statistical
significance
(p
<
0.05)
nine
elbows.
Correlation
analyses
proved
associations
between
ROM
GM-CSF
concentrations
28).
TNF-
α
showed
negative
correlation
at
28,
while
IL-10
demonstrated
positive
ROM.
As
consequence
A-MSC
application
joint,
we
detected
statistically
significant
decrease
concentration
levels
TNF-α
increase
IL-10.
Statistical
well
group.
IL-6
Journal of Leukocyte Biology,
Journal Year:
2024,
Volume and Issue:
unknown
Published: April 17, 2024
Abstract
The
treatment
of
nonhealing
wounds,
such
as
diabetic
ulcers,
remains
a
critical
clinical
challenge.
Recent
breakthroughs
in
cell
therapy
have
shown
great
promise,
with
one
primary
focus
on
preparing
cells
comprehensive
reparative
functions
and
foreseeable
safety.
In
our
previous
study,
we
recapitulated
the
proregenerative
immunosuppressive
tumor-associated
macrophages
non-tumor-derived
macrophages,
endowing
latter
characteristics
for
promoting
wound
healing—termed
macrophage–educated
macrophages.
To
eliminate
use
tumor-derived
sources
devise
more
controllable
method
to
prepare
macrophage–like
cells,
this
identify
cocktail
comprising
5
recombinant
proteins
an
essential
condition
induce
nonpolarized
into
therapeutic
prohealing
functions.
screened
factors
are
osteopontin,
macrophage
inflammatory
protein
2,
chemokine
(C-C
motif)
ligand
8,
vascular
endothelial
growth
factor
B,
colony-stimulating
factor.
We
demonstrate
rationale
screening
these
phenotype
factor-induced
macrophage-educated
macrophage-like
prepared
from
murine
bone
marrow–derived
which
exhibit
angiogenic
immunomodulatory
effects
vitro.
Then,
human
monocytes
periphery
blood
show
cell-based
ex
vivo
model
(T-Skin™).
Our
study
demonstrates
simple,
effective,
approach
possess
repairing
activities,
may
provide
insights
developing
therapeutics
wounds
clinically.
Journal of Stem Cells and Regenerative Medicine,
Journal Year:
2024,
Volume and Issue:
unknown
Published: May 31, 2024
Osteoarthritis
(OA)
is
a
degenerative
disease
that
causes
chronic
pain
and
disability
worldwide.
This
mainly
caused
by
IL-1β
TNF-α,
which
lead
to
cartilage
degradation
inhibit
the
repair
capacity
of
damaged
cartilage.
Recent
studies
have
shown
amniotic
fluid
mesenchymal
stem
cells
(AF-MSCs)
secrete
proteins
can
effectively
help
in
treatment
OA.
However,
underlying
mechanism
still
unclear.
Therefore,
aim
this
study
was
investigate
effects
mechanisms
behind
healing
properties
AF-MSC
secretome
(AFS-se)
under
OA
conditions.
involved
growing
chondrocyte
progenitor
(CPCs)
traumatized
tissues
presence
cytokines
mimic
AFS-se
then
added
culture
medium
determine
its
effect
on
CPCs
Cell
migration,
endogenous
cell
outgrowth,
expression
chondrogenic
anabolic
genes,
NF-κB
MAPK
signaling
pathways
were
examined
study.
inhibited
inflammatory
TNF-α
significantly
reducing
ERK
phosphorylation
pathway
decreasing
downstream
proinflammatory
COX2
products.
The
impaired
recovered
their
ability
migrate,
injured
osteoarthritic
able
regrow
response
stimuli.
Additionally,
genes
such
as
