Applications of 3D organoids in toxicological studies: a comprehensive analysis based on bibliometrics and advances in toxicological mechanisms

Archives of Toxicology, Journal Year: 2024, Volume and Issue: 98(8), P. 2309 - 2330

Published: May 28, 2024

Language: Английский

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Advancing Brain Organoid Electrophysiology: Minimally Invasive Technologies for Comprehensive Characterization

Advanced Materials Technologies, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

Abstract Human brain organoids, which originate from pluripotent stem cells, serve as valuable tools for a wide range of research endeavors, replicating function. Their capacity to replicate cellular interactions, morphology, and division provides invaluable insights into development, disease modeling, drug screening. However, conventional morphological analysis methods are often invasive lack real‐time monitoring capabilities, posing limitations achieving comprehensive understanding. Therefore, advancing the comprehension organoid electrophysiology necessitates development minimally measurement technologies with long‐term, high‐resolution capabilities. This review highlights significance human organoids emphasizes need electrophysiological characterization. It delves assessment methods, particularly focusing on 3D microelectrode arrays, electrode insertion mechanisms, importance flexible arrays facilitate recordings. Additionally, various sensors tailored monitor properties introduced, enriching understanding their chemical, thermal, mechanical dynamics.

Language: Английский

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Two- and Three-Dimensional In Vitro Models of Parkinson’s and Alzheimer’s Diseases: State-of-the-Art and Applications

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 620 - 620

Published: Jan. 13, 2025

In vitro models play a pivotal role in advancing our understanding of neurodegenerative diseases (NDs) such as Parkinson’s and Alzheimer’s disease (PD AD). Traditionally, 2D cell cultures have been instrumental elucidating the cellular mechanisms underlying these diseases. Cultured cells derived from patients or animal provide valuable insights into pathological processes at level. However, they often lack native tissue environment complexity, limiting their ability to fully recapitulate features. contrast, 3D offer more physiologically relevant platform by mimicking brain architecture. These can incorporate multiple types, including neurons, astrocytes, microglia, creating microenvironment that closely resembles brain’s complexity. Bioengineering approaches allow researchers better replicate cell–cell interactions, neuronal connectivity, disease-related phenotypes. Both advantages limitations. While simplicity scalability for high-throughput screening basic processes, enhanced physiological relevance Integrating findings both model systems NDs, ultimately aiding development novel therapeutic strategies. Here, we review existing study PD AD.

Language: Английский

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Characterizing Oxidative Stress induced by Aβ Oligomers and the Protective Role of Carnosine in Primary Mixed Glia Cultures

Free Radical Biology and Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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State-of the-art and future perspective in co-culture systems for tendon engineering

Biomaterials and Biosystems, Journal Year: 2025, Volume and Issue: 17, P. 100110 - 100110

Published: March 1, 2025

Tendon is a connective tissue that links bone to muscle, allowing for maintenance of skeleton posture, joint movement, energy storage and transmission muscle force bone. hypocellular hypovascular poor self-regeneration capacity. Current surgical treatments are limited success, frequently resulting in reinjury. Upcoming cell therapies primarily based on tenocytes, population self-renewal capacity vitro or mesenchymal stromal cells, prone ectopic formation vivo. Over the years mono- multi- factorial culture technologies have failed effectively maintain tenocyte phenotype during expansion prime cells towards tenogenic lineage prior implantation. Upon these limitations concept co-culture was conceived. Here, we comprehensively review discuss differentiation through direct indirect with tenocytes an attempt generate tendon-like regenerative medicine purposes.

Language: Английский

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Regulation of keratinocyte proliferation and differentiation by secoiridoid oleacein in monoculture and fibroblast co-culture models

Biomedicine & Pharmacotherapy, Journal Year: 2025, Volume and Issue: 185, P. 117985 - 117985

Published: March 14, 2025

Language: Английский

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Thyroid cancer: Current status of preclinical landscape

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 115 - 138

Published: Jan. 1, 2025

Language: Английский

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Adipose derived stem cells – Sources, differentiation capacity and a new target for reconstructive and regenerative medicine

Biomedicine & Pharmacotherapy, Journal Year: 2025, Volume and Issue: 186, P. 118036 - 118036

Published: April 8, 2025

Language: Английский

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HA-PDL Composite Hydrogel with Self-Regulating Elastic Modulus for FRESH 3D Printing of Neural Cell Model

Colloids and Surfaces A Physicochemical and Engineering Aspects, Journal Year: 2025, Volume and Issue: unknown, P. 136916 - 136916

Published: April 1, 2025

Language: Английский

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Ginsenoside Rg1 controls CKLF1-mediated apoptosis to reduce hypoxic/reoxygenation injury in HT22 cells

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 29, 2025

Background Stroke is a prevalent and debilitating neurodegenerative condition. Ginsenoside Rg1 has demonstrated neuroprotective properties in the context of stroke. The upregulation chemokine-like factor 1 (CKLF1) observed ischemic stroke positions CKLF1 as promising therapeutic target. However, limited research explored whether can mitigate oxygen-glucose deprivation/reoxygenation (OGD/R)-induced apoptosis HT22 cells through modulation CKLF1. Methods In this study, Na 2 S O 4 was used to treat establish OGD/R model. effects different concentrations on cell viability were firstly determined by CCK-8 assay determine its safe administration range. Subsequently, level oxidative stress assessed detecting LDH release antioxidant indexes (CAT, SOD, MDA). Western blotting analyse expression apoptosis-related proteins, TUNEL staining quantify rate. To explore cell-cell interactions, Transwell co-culture system BV-2 established. Results optimal parameters for model determined: 25 mmol/L treatment 2.5 h followed reoxygenation, inoculation density × 10 5 cells/mL day culture. Based safety assessment, 5, 25, 50 μmol/L selected intervention. significantly decreased ( P ≤ 0.05) MDA content alleviated stress. showed that dose-dependently downregulated inhibited Caspase-3 other apoptotic protein activation. HT22/BV-2 system, microglia activation, shown reduced NO IL-1β secretion 0.05). Conclusion attenuates injury, reduces inhibiting alleviates inflammatory response activated cells, showing potential.

Language: Английский

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