Investigating Development and Defense Systems in Early Reproductive Stages of Male and Female Gonads in Black Scorpionfish Scorpaena porcus (Linnaeus, 1758) DOI Creative Commons
Alessio Alesci, Sebastián Marino, Claudio D’Iglio

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(8), P. 587 - 587

Published: Aug. 2, 2024

One of the most crucial biological indicators in tracking long-term variations reproductive cycle is sexual development. Scorpaena porcus (Linnaeus, 1758), commonly known as black scorpionfish, a small teleost from family Scorpaenidae. Much about its ecology, but data on and defense systems are still lacking. Antimicrobial peptides (AMPs), such piscidins, integral components innate immune system fish. These exhibit wide range activity against bacteria, fungi, viruses, protozoa act first line host defense. This study aims to investigate primary development stages male female gonads providing additional knowledge biology this while evaluating concomitant changes expression Piscidin-1 antimicrobial peptide. The results show histological, morpho-structural change immature stage developing virgin stage. Immunohistochemical analyses that germinal somatic cells strongly reactive both at an early ontogeny suggest may play key role local scorpionfish delicate stage, which critical for continuation maintenance species. present findings potentially useful better understanding fish, improving our interaction between reproduction.

Language: Английский

Internal Defense System of Mytilus galloprovincialis (Lamarck, 1819): Ecological Role of Hemocytes as Biomarkers for Thiacloprid and Benzo[a]Pyrene Pollution DOI Creative Commons
Alessio Alesci,

Davide Di Paola,

Angelo Fumia

et al.

Toxics, Journal Year: 2023, Volume and Issue: 11(9), P. 731 - 731

Published: Aug. 25, 2023

The introduction of pollutants, such as thiacloprid and benzo[a]pyrene (B[a]P), into the waters urbanized coastal estuarine areas through fossil fuel spills, domestic industrial waste discharges, atmospheric inputs, continental runoff poses a major threat to fauna flora aquatic environment can have significant impact on internal defense system invertebrates mussels. Using monoclonal polyclonal anti-Toll-like receptor 2 (TLR2) anti-inducible nitric oxide synthetase (iNOS) antibodies for first time, this work aims examine hemocytes in mantle gills M. galloprovincialis biomarkers B[a]P pollution analyze their potential synergistic effect. To pursue objective, samples were exposed both individually simultaneously. Subsequently, oxidative stress evaluated by enzymatic analysis, while tissue changes number different contaminated groups assessed via histomorphological immunohistochemical analyses. Our findings revealed that comparison single exposure, two pollutants together significantly elevated stress. Moreover, our data may potentially enhance knowledge how TLR2 iNOS part bivalves. This would help creating new technologies strategies, biosensors, are more suitable managing water pollution, garnering details condition marine ecosystem.

Language: Английский

Citations

4

Advancements of fish-derived peptides for mucormycosis: a novel strategy to treat diabetic compilation DOI
Kadhirmathiyan Velumani, Abirami Arasu, Praveen Kumar Issac

et al.

Molecular Biology Reports, Journal Year: 2023, Volume and Issue: 50(12), P. 10485 - 10507

Published: Nov. 2, 2023

Language: Английский

Citations

4

A comparison of teleost rodlet cells with apicomplexan cells DOI
Joseph A. DePasquale

Acta Histochemica, Journal Year: 2024, Volume and Issue: 126(4), P. 152167 - 152167

Published: May 1, 2024

Language: Английский

Citations

1

Mechanosensory cells in annelid oligochaete Lumbricus terrestris (Linnaeus, 1758): A new insight on worm evolution DOI
Alessio Alesci, Emmanuele Messina, Krystyna Żuwała

et al.

Acta Zoologica, Journal Year: 2023, Volume and Issue: 105(3), P. 331 - 339

Published: June 18, 2023

Abstract Ciliated and non‐ciliated mechanosensory cells in invertebrates have intricate cytoskeletal structures that, combined with microtubules, act as a mechanical link between external stimulus signal processing. As result, they can perceive forces like touch, cuticle deformation, gravity sound. Through the expression of antibodies against serotonin (5‐HT), calbindin, inducible nitric oxide synthase (iNOS) α‐smooth muscle actin (αSMA), this research aims to investigate integument Lumbricus terrestris (Linnaeus, 1758) an evolutionary perspective. In epidermis, we discovered isolated that were immunopositive every antibody examined. Our findings improve knowledge evolution annelid sensory biology adding new insights on transduction help better understand morpho‐structural adaptations invertebrate skin scale give more taxonomic data for species distinction.

Language: Английский

Citations

3

Exploring the Potential of Isalo Scorpion Cytotoxic Peptide in Enhancing Gill Barrier Function and Immunity in Grass Carp (Ctenopharyngodon idella) Infected with Flavobacterium columnare DOI Creative Commons
Qiuyan Chen,

Qi-Yu Hu,

Wei‐Dan Jiang

et al.

Aquaculture Nutrition, Journal Year: 2024, Volume and Issue: 2024(1)

Published: Jan. 1, 2024

The objective of this research was to investigate how dietary antimicrobial peptides (AMP), namely, Isalo scorpion cytotoxic peptide (IsCT), affect the gill physical barrier function and immune grass carp challenged with Flavobacterium columnare ( F. ). Five hundred forty were randomly allocated six groups fed varying levels IsCT in diet (0, 0.6, 1.2, 1.8, 2.4, 3.0 mg/kg diet) for a duration 60 days. Afterward, carps faced challenge from . results revealed that use optimal dramatically mitigated damage infected Additionally, exhibited notable enhancement antioxidant capabilities, as evidenced by significant reduction ROS, MDA, PC levels, an elevation enzyme activities, upregulation antioxidant‐related genes Nrf2 mRNA levels. Conversely, expression Keap1a Keap1b decreased. Besides, its capability inhibit apoptosis via downregulating caspase-2 , caspase-3 caspase-7 caspase-8 caspase-9 Apaf1 Fasl Bax JNK while concurrently increasing Bcl-2 Mcl-1 IAP fish gills. promoted integrity tight junction gene claudin-b claudin-c claudin-3c ZO-1 ZO-2b occludin JAM suppressing MLCK signaling. improved antibacterial ability, heightened LZ, ACP elevated C3, C4, IgM. there β-defensin-1 LEAP-2A LEAP-2B hepcidin mucin-2 Simultaneously, inclusion resulted immunity barriers through proinflammatory cytokine increase anti‐inflammatory This partly facilitated I κ Ba/NF‐ B p65 signaling pathway TOR/S6K1 pathways gills carp. Therefore, supplementing has potential advantages enhancing health Based on LZ activity against lipid peroxidation, optimum concentrations on‐growing (136.88 ± 0.72 g) found be 1.68 1.54 diet, respectively.

Language: Английский

Citations

0

Investigating Development and Defense Systems in Early Reproductive Stages of Male and Female Gonads in Black Scorpionfish Scorpaena porcus (Linnaeus, 1758) DOI Creative Commons
Alessio Alesci, Sebastián Marino, Claudio D’Iglio

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(8), P. 587 - 587

Published: Aug. 2, 2024

One of the most crucial biological indicators in tracking long-term variations reproductive cycle is sexual development. Scorpaena porcus (Linnaeus, 1758), commonly known as black scorpionfish, a small teleost from family Scorpaenidae. Much about its ecology, but data on and defense systems are still lacking. Antimicrobial peptides (AMPs), such piscidins, integral components innate immune system fish. These exhibit wide range activity against bacteria, fungi, viruses, protozoa act first line host defense. This study aims to investigate primary development stages male female gonads providing additional knowledge biology this while evaluating concomitant changes expression Piscidin-1 antimicrobial peptide. The results show histological, morpho-structural change immature stage developing virgin stage. Immunohistochemical analyses that germinal somatic cells strongly reactive both at an early ontogeny suggest may play key role local scorpionfish delicate stage, which critical for continuation maintenance species. present findings potentially useful better understanding fish, improving our interaction between reproduction.

Language: Английский

Citations

0