Biology Direct, Journal Year: 2025, Volume and Issue: 20(1)
Published: April 2, 2025
Language: Английский
International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(5), P. 4914 - 4914
Published: March 3, 2023
The endoplasmic reticulum is an organelle exerting crucial functions in protein production, metabolism homeostasis and cell signaling. Endoplasmic stress occurs when cells are damaged the capacity of this to perform its normal reduced. Subsequently, specific signaling cascades, together forming so-called unfolded response, activated deeply impact fate. In renal cells, these molecular pathways strive either resolve injury or activate death, depending on extent damage. Therefore, activation pathway was suggested as interesting therapeutic strategy for pathologies such cancer. However, cancer known hijack mechanisms exploit them their advantage order promote survival through rewiring metabolism, oxidative responses, autophagy, inhibition apoptosis senescence. Recent data strongly suggest that a certain threshold needs be attained shift responses from pro-survival pro-apoptotic outcome. Several pharmacological modulators interest purposes already available, but only handful were tested case carcinoma, effects vivo setting remain poorly known. This review discusses relevance suppression progression potential targeting cellular process
Language: Английский
Citations
16
BMC Cancer, Journal Year: 2024, Volume and Issue: 24(1)
Published: April 11, 2024
Abstract Clear cell renal carcinoma (ccRCC) is the prevailing histological subtype of and has unique metabolic reprogramming during its occurrence development. Cell senescence one newly identified tumor characteristics. However, there a dearth methodical all-encompassing investigations regarding correlation between broad-ranging alterations in processes associated with aging ccRCC. We utilized range analytical methodologies, such as protein‒protein interaction network analysis least absolute shrinkage selection operator (LASSO) regression analysis, to form validate risk score model known senescence-metabolism-related (SeMRM). Our study demonstrated that SeMRM could more precisely predict OS ccRCC patients than clinical prognostic markers use. By utilizing two distinct datasets ccRCC, ICGC-KIRC (the International Cancer Genome Consortium) GSE29609, well single-cell dataset (GSE156632) real patient information, further confirmed relationship (SeMRS) progression. It worth noting who were classified into different subgroups based on SeMRS exhibited notable variations activity, immune microenvironment, type transformation, mutant landscape, drug responsiveness. also PTGER4, key gene SeMRM, regulated proliferation, lipid levels cycle vivo vitro. Together, utilization potential function dependable characteristic increase accuracy assessment for diagnosed thereby facilitating suitable treatment strategies.
Language: Английский
Citations
5
Cancer Reports, Journal Year: 2024, Volume and Issue: 7(6)
Published: June 1, 2024
Abstract Clear cell renal carcinoma (ccRCC) is a common and aggressive subtype of kidney cancer. Many patients are diagnosed at advanced stages, making early detection crucial. Unfortunately, there currently no noninvasive tests for ccRCC, emphasizing the need new biomarkers. Additionally, ccRCC often develops resistance to treatments like radiotherapy chemotherapy. Identifying biomarkers that predict treatment outcomes vital personalized care. The integration artificial intelligence (AI), multi‐omics analysis, computational biology holds promise in bolstering precision resilience, opening avenues future investigations. amalgamation radiogenomics biomaterial‐basedimmunomodulation signifies revolutionary breakthrough diagnostic medicine. This review summarizes existing literature highlights emerging enhance diagnostic, predictive, prognostic capabilities setting stage clinical research.
Language: Английский
Citations
5
Cancers, Journal Year: 2024, Volume and Issue: 16(2), P. 391 - 391
Published: Jan. 17, 2024
While the transmembrane glycoprotein mucin 1 (MUC1) is clustered at apical borders of normal epithelial cells, with transformation and loss polarity, MUC1 found high levels in cytosol uniformly distributed over entire surface carcinoma where it can promote tumor progression adversely affects response to therapy. Clear cell renal (ccRCC), main histotype kidney cancer, typically highly resistant conventional targeted therapies for reasons that remain largely unknown. In this context, we investigated whether also plays a pivotal role cellular molecular events driving ccRCC chemoresistance. We showed, using loss- gain-of-function approaches ccRCC-derived lines, not only influences but induces multi-drug-resistant profile reminiscent activation ABC drug efflux transporters. Overall, our results suggest targeting may represent novel therapeutic approach limit improve sensitivity.
Language: Английский
Citations
4
BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)
Published: April 26, 2024
Abstract Background Disulfidptosis is a novel form of programmed cell death induced by high SLC7A11 expression under glucose starvation conditions, unlike other known forms death. However, the roles disulfidptosis in cancers have yet to be comprehensively well-studied, particularly ccRCC. Methods The profiles and somatic mutation DGs from TCGA database were investigated. Two clusters identified unsupervised consensus clustering analysis, disulfidptosis-related prognostic signature (DR score) was constructed. Furthermore, predictive capacity DR score prognosis validated several clinical cohorts. We also developed nomogram based on features. Then, we investigated differences clinicopathological information, TMB, tumor immune landscapes, biological characteristics between high- low-risk groups. evaluated whether robust tool for predicting immunotherapy response TIDE algorithm, checkpoint genes, submap CheckMate cohort. Results two with significant prognosis, has been demonstrated as an independent risk factor high-risk group patients had more complicated microenvironment suffered evasion immunotherapy. Moreover, better immunotherapy, anti-PD1 anti-CTLA-4 inhibitors, which verified Conclusion can accurately predict assist clinicians providing personalized treatment regime ccRCC patients.
Language: Английский
Citations
4
Metabolomics, Journal Year: 2024, Volume and Issue: 20(1)
Published: Jan. 24, 2024
Language: Английский
Citations
3
Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: April 23, 2024
Abstract The advent of micro-physiological systems (MPS) in biomedical research has enabled the introduction more complex and relevant physiological into vitro models. recreation morphological features three-dimensional environments can recapitulate otherwise absent dynamic interactions conventional In this study we developed an advanced Renal Cell Carcinoma (RCC) that mimics interplay between healthy malignant renal tissue. Based on TissUse Humimic platform our model combines proximal tubule epithelial cells (RPTEC) RCC. Co-culturing reconstructed RPTEC tubules with RCC spheroids a closed micro-perfused circuit resulted significant phenotypical changes to tubules. Expression immune factors revealed interleukin-8 (IL-8) tumor necrosis factor-alfa (TNF-α) were upregulated non-malignant while neutrophil gelatinase-associated lipocalin (NGAL) was downregulated both RPTEC. Metabolic analysis showed prompted shift energy production tubules, inducing glycolysis, metabolic adaptation likely supports growth immunogenicity. contrast, maintained stable activity, emphasizing their resilience external factors. RNA-seq biological process primary RTPTEC demonstrated 3D tubular architecture MPS conditions reverted predominant oxidative phosphorylate state, departure from glycolytic metabolism observed 2D culture. This co-culture model, approximates physiology RCC, providing new insights tumor-host interactions. Our approach show RCC-MPS expand complexity scope pathophysiology biomarker studies kidney cancer research.
Language: Английский
Citations
3
Translational research, Journal Year: 2024, Volume and Issue: 271, P. 1 - 12
Published: April 24, 2024
Language: Английский
Citations
3
Journal of Advanced Research, Journal Year: 2024, Volume and Issue: unknown
Published: May 1, 2024
Renal cell carcinoma (RCC) is one of the most common malignant tumors urinary system and accounts for more than 90 % all renal tumors. Resistance to targeted therapy has emerged as a pivotal factor that contributes progressive deterioration patients with advanced RCC. Metabolic reprogramming hallmark tumorigenesis progression, an increasing body evidence indicating abnormal lipid metabolism plays crucial role in advancement clear carcinoma. Clarify precise mechanisms underlying drug resistance. Bioinformatics screening analyses were performed identify hub gene. qRT-PCR, western blot, chromatin immunoprecipitation (ChIP) assays, other biological methods used explore verify related pathways. Various line models animal perform functional experiments. In this study, we identified Mesoderm induction early response 2 (MIER2) novel biomarker RCC, demonstrating its promoting malignancy sunitinib resistance by influencing Mechanistically, MIER2 facilitated P53 deacetylation binding HDAC1. Acetylation modification augmented stability transcriptional function P53, while hindered process PGC1A, leading intracellular accumulation Furthermore, Trichostatin A (TSA), inhibitor HDAC1, was found impede MIER2/HDAC1/P53/PGC1A pathway, offering potential benefits sunitinib-resistant cancer. Our findings highlight key player anchoring HDAC1 inhibiting PGC1A expression through thereby inducing RCC Lipid-rich cells compensate energy production sustain their own growth glycolysis-independent manner, evading cytotoxic effects drugs ultimately culminating development
Language: Английский
Citations
3
Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)
Published: Jan. 9, 2025
Clear cell renal carcinoma (ccRCC) is resistant to radiotherapy and chemotherapy. Thus, it necessary find new diagnostic markers therapeutic targets increase the overall outcomes of ccRCC. Recent studies have shown that methods interfere with energy transfer system can also positively affect treatment process. The present study focused on finding associated mitochondrial metabolic pathways outcome For this purpose, we investigated various aspects relationship between metabolism ccRCC based analysis gene network connections differentially expressed genes, through assessment protein–protein interaction, mutations, promoter methylation related genes. We interaction miRNAs immune infiltration analysis. Through these steps, provided a list possible for current further proved importance in pathogenesis from be used
Language: Английский
Citations
0