Mass spectrometry-based proteomics as an emerging tool in clinical laboratories

Clinical Proteomics, Journal Year: 2023, Volume and Issue: 20(1)

Published: Aug. 26, 2023

Abstract Mass spectrometry (MS)-based proteomics have been increasingly implemented in various disciplines of laboratory medicine to identify and quantify biomolecules a variety biological specimens. MS-based is continuously expanding widely applied biomarker discovery for early detection, prognosis markers treatment response prediction monitoring. Furthermore, making these advanced tests more accessible affordable will the greatest healthcare benefit. This review article highlights new paradigms clinical has created microbiology laboratories, cancer research diagnosis metabolic disorders. The technique preferred over conventional methods disease detection therapy monitoring its combined advantages multiplexing capacity, remarkable analytical specificity sensitivity low turnaround time. Despite achievements development adoption number practices, are expected undergo transition from bench bedside near future. provides insights trials recent progresses (mainly covering literature NCBI database) application laboratories.

Language: Английский

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Progress in Understanding Metabolic Syndrome and Knowledge of Its Complex Pathophysiology

Diabetology, Journal Year: 2023, Volume and Issue: 4(2), P. 134 - 159

Published: April 12, 2023

The metabolic syndrome (MetS), first introduced by Haller in 1975, was sometimes also known as insulin resistance syndrome, X, and plurimetabolic syndrome. In 1989, it rechristened Kaplan the “Deadly Quartet” based on a consolidation of central obesity, impaired glucose tolerance, dyslipidemia, systemic hypertension. MetS is positively associated with pro-inflammatory pro-thrombotic state, attributed to increased inflammatory marker activity. Moreover, frequently atherosclerotic cardiovascular disease, hyperuricemia, obstructive sleep apnea, chronic kidney disease. Despite concerted endeavors worldwide, complexity pathophysiology still needs be clearly understood. Currently, therapeutic possibilities are confined individual therapy for hyperglycemia, hypertension, hypertriglyceridemia, regular physical exercise, restricted diet. this review, progress regarding understanding MetS; recent emerging technologies, such metabolomics proteomics; relation diabetes, diseases; association COVID-19 discussed.

Language: Английский

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Unveiling the future of metabolic medicine: omics technologies driving personalized solutions for precision treatment of metabolic disorders

Biochemical and Biophysical Research Communications, Journal Year: 2023, Volume and Issue: 682, P. 1 - 20

Published: Sept. 29, 2023

Language: Английский

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Using metabolomics and proteomics to identify the potential urine biomarkers for prediction and diagnosis of gestational diabetes

EBioMedicine, Journal Year: 2024, Volume and Issue: 101, P. 105008 - 105008

Published: Feb. 17, 2024

Gestational diabetes mellitus (GDM) is one of the most common metabolic complications during pregnancy, threatening both maternal and fetal health. Prediction diagnosis GDM not unified. Finding effective biomarkers for particularly important achieving early prediction, accurate timely intervention. Urine, due to its accessibility in large quantities, noninvasive collection easy preparation, has become a good sample biomarker identification. In recent years, number studies using metabolomics proteomics approaches have identified differential expressed urine metabolites proteins patients. this review, we summarized these potential prediction elucidated their role development GDM.

Language: Английский

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Volatile Organic Compound–Drug Receptor Interactions: A Potential Tool for Drug Design in the Search for Remedies for Increasing Toxic Occupational Exposure

Processes, Journal Year: 2025, Volume and Issue: 13(1), P. 154 - 154

Published: Jan. 8, 2025

Volatile organic compounds (VOCs) can impact the actions of drugs due to their effects on drug receptors and activities enzymes involved in various metabolic processes, especially those relating gene regulation. They disrupt cellular functions potentially affect human metabolism utilization receptors. mimic or inhibit endogenous ligands, leading carcinogenesis, neurotoxicity, endocrine disruption, respiratory disorders. Chronic exposure VOCs occupation lead an increased generation reactive oxygen species (ROS), which could oxidative stress damage lipids, affecting formation proper functioning regulation, enzyme activity, cell membranes. The presence interfere with activity body’s ability process utilize effectively. This is because such as antioxidant play essential role protection against damage. Therefore, disruptions distort overall health condition through breakdown defense mechanisms. In this study, aim assess effect VOC way forward designing maintaining optimal for workers’ well-being.

Language: Английский

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Reduced Dietary Protein Induces Changes in the Dental Proteome

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

Abstract Experimental studies have demonstrated that nutritional changes during development can result in phenotypic to mammalian cheek teeth. This developmental plasticity of tooth morphology is an example plasticity. Because occurs through complex interactions between manifold processes, there are many potential mechanisms which contribute a tooth’s norm reaction. Determining the identity those and relative importance each them one main challenges understanding Quantitative proteomics combined with experimental allow for identification molecular contributors plastic response quantification expressed gene products. Here, we present results quantitative analysis mature upper first molars (M1s) Mus musculus from controlled feeding experiment. Pregnant nursing mothers were fed either low-dietary protein (10%) treatment diet or control (20%) diet. Expression tooth-related proteins, immune system actin-based myosin proteins significantly altered our sample. The recovery expression change was anticipated consistent previous proteomic studies. We also identified differential along systematic reduction expression, novel discoveries propose aim elucidate specific molar should prioritize investigations into relationships IGF regulation development. Research Highlights A low-protein major dental building within . Graphical

Language: Английский

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Plasmid Gene Therapy for Monogenic Disorders: Challenges and Perspectives

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(1), P. 104 - 104

Published: Jan. 14, 2025

Monogenic disorders are a group of human diseases caused by mutations in single genes. While some disease-altering treatments offer relief and slow the progression certain conditions, majority monogenic still lack effective therapies. In recent years, gene therapy has appeared as promising approach for addressing genetic disorders. However, despite advancements manipulation tools delivery systems, several challenges remain unresolved, including inefficient delivery, sustained expression, immunogenicity, toxicity, capacity limitations, genomic integration risks, limited tissue specificity. This review provides an overview plasmid-based techniques methods currently employed diseases, highlighting they face exploring potential strategies to overcome these barriers.

Language: Английский

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Omics and rare diseases: challenges, applications, and future perspectives

Expert Review of Proteomics, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 16, 2025

Introduction Rare diseases (RDs) are a heterogeneous group of recognized as relevant global health priority but posing aspects complexity such as: geographical scattering affected individuals, improper/late diagnosis, limited awareness, difficult surveillance and monitoring, understanding natural history, lack treatment. Usually, RDs have pediatric onset life-long, multisystemic, associated with poor prognosis.

Language: Английский

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International Precision Child Health Partnership (IPCHiP): an initiative to accelerate discovery and improve outcomes in rare pediatric disease

npj Genomic Medicine, Journal Year: 2025, Volume and Issue: 10(1)

Published: Feb. 27, 2025

Advances in genomic technologies have revolutionized the diagnosis of rare genetic diseases, leading to emergence precision therapies. However, there remains significant effort ahead ensure promise medicine translates improved outcomes. Here, we discuss challenges advancing child health and highlight how international collaborations such as International Precision Child Health Partnership, which embed research into clinical care, can maximize benefits for children globally.

Language: Английский

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Review: Utility of mass spectrometry in rare disease research and diagnosis

npj Genomic Medicine, Journal Year: 2025, Volume and Issue: 10(1)

Published: March 31, 2025

Individuals affected by a rare disease often experience long and arduous diagnostic odyssey. Delivery of genetic answers in timely manner is critical to individuals their families. Multi-omics, term which usually encompasses genomics, transcriptomics, proteomics, metabolomics lipidomics, has gained increasing popularity research diagnosis over the past decade. Mass spectrometry (MS) technique allowing study proteins, metabolites lipids fragments at scale, enabling researchers effectively determine presence abundance thousands molecules single test, accurately quantify specific levels, identify potential therapeutic biomarkers, detect differentially expressed proteins patients with diseases, monitor progression treatment response. In this review, we focus on mass (MS)-based omics survey literature describing utility different MS-based how they have transformed diagnosis.

Language: Английский

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