NRICM101 in combatting COVID-19 induced brain fog: Neuroprotective effects and neurovascular integrity preservation in hACE2 mice DOI Creative Commons
Cher-Chia Chang, Yea‐Hwey Wang, Jiin‐Cherng Yen

et al.

Journal of Traditional and Complementary Medicine, Journal Year: 2024, Volume and Issue: 15(1), P. 36 - 50

Published: July 3, 2024

Amidst growing concerns over COVID-19 aftereffects like fatigue and cognitive issues, NRICM101, a traditional Chinese medicine, has shown promise. Used by 2 million people globally, it notably reduces hospitalizations intubations in patients. To explore whether NRICM101 could combat brain fog, we tested on hACE2 transgenic mice administered the S1 protein of SARS-CoV-2, aiming to mitigate S1-induced issues measuring animal behaviors, immunohistochemistry (IHC) staining, next-generation sequencing (NGS) analysis. The study revealed that protein-administered displayed marked signs characterized reduced learning, memory, nesting abilities. However, treatment these animals ameliorated all functions. administration induced notable inflammation, leading death neurons (NeuN+) neural stem cells (DCX+) mice. This was accompanied heightened microglia activation (IBA1+/CD68+), increased cytokine production (IL1β, IL6), induction neutrophil extracellular traps (NET), inflammation (NLRP3, CD11b), platelet (CD31, vWF) complement (C3) activation, ultimately damaging neurovasculature disrupting blood-brain barrier (B.B.B.). Administration effectively alleviated pathological changes. In conclusion, potential prevent COVID-19-associated fog bolstering neurovascular integrity protecting cells. is achieved inhibition protein-induced which turn leads prevention damage subsequent neurons.

Language: Английский

NRICM101 prevents kainic acid-induced seizures in rats by modulating neuroinflammation and the glutamatergic system DOI Creative Commons
Chi‐Feng Hung, Wei‐Che Chiu,

Jia-Cih Chen

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 140, P. 112842 - 112842

Published: Aug. 1, 2024

Taiwan Chingguan Yihau (NRICM101) is a Traditional Chinese medicine (TCM) formula used to treat coronavirus disease 2019; however, its impact on epilepsy has not been revealed. Therefore, the present study evaluated anti-epileptogenic effect of orally administered NRICM101 kainic acid (KA)-induced seizures in rats and investigated possible mechanisms action. Sprague-Dawley were (300 mg/kg) by oral gavage for 7 consecutive days before receiving an intraperitoneal injection KA (15 mg/kg). considerably reduced seizure behavior electroencephalographic induced rats. also significantly decreased neuronal loss glutamate increase increased GLAST, GLT-1, GAD67, GDH GS levels cortex hippocampus KA-treated In addition, suppressed astrogliosis (as determined GFAP expression); neuroinflammatory signaling HMGB1, TLR-4, IL-1β, IL-1R, IL-6, p-JAK2, p-STAT3, TNF-α, TNFR1 p-IκB levels, cytosolic p65-NFκB levels); necroptosis p-RIPK3 p-MLKL levels) The effects similar those carbamazepine, well-recognized antiseizure drug. Furthermore, no toxic liver kidney observed NRICM101-treated results indicate that antiepileptogenic neuroprotective through suppression inflammatory cues (HMGB1/TLR4, Il-1β/IL-1R1, IL-6/p-JAK2/p-STAT3, TNF-α/TNFR1/NF-κB) pathways (TNF-α/TNFR1/RIP3/MLKL) associated with level regulation brain innocuous. Our findings highlight promising role management epilepsy.

Language: Английский

Citations

4
Bioinformatics-based investigation on the genetic influence between SARS-CoV-2 infections and idiopathic pulmonary fibrosis (IPF) diseases, and drug repurposing DOI Creative Commons
Md. Ariful Islam, Md. Kaderi Kibria, Md. Bayazid Hossen

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: March 22, 2023

Abstract Some recent studies showed that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections and idiopathic pulmonary fibrosis (IPF) disease might stimulate each other through the shared genes. Therefore, in this study, an attempt was made to explore common genomic biomarkers for SARS-CoV-2 IPF highlighting their functions, pathways, regulators associated drug molecules. At first, we identified 32 statistically significant differentially expressed genes (cDEGs) between (SARS-CoV-2 IPF) control samples of RNA-Seq profiles by using a statistical r-package (edgeR). Then detected 10 cDEGs (CXCR4, TNFAIP3, VCAM1, NLRP3, TNFAIP6, SELE, MX2, IRF4, UBD CH25H) out as hub (cHubGs) protein–protein interaction (PPI) network analysis. The cHubGs regulatory analysis few key TFs-proteins miRNAs transcriptional post-transcriptional cHubGs. cDEGs-set enrichment some crucial causing molecular mechanisms including biological processes, cellular components signaling pathways. Then, suggested cHubGs-guided top-ranked candidate molecules (Tegobuvir, Nilotinib, Digoxin, Proscillaridin, Simeprevir, Sorafenib, Torin 2, Rapamycin, Vancomycin Hesperidin) treatment against with IFP diseases comorbidity. Finally, investigated resistance performance our proposed compare already published molecules, state-of-the-art alternatives publicly available independent receptors docking Molecular results would be more effective Thus, findings study played vital role diagnosis therapies comorbidity risk.

Language: Английский

Citations

10
Computational identification of differentially-expressed genes as suggested novel COVID-19 biomarkers: A bioinformatics analysis of expression profiles DOI Creative Commons
Valentina Di Salvatore, Elena Crispino, Avisa Maleki

et al.

Computational and Structural Biotechnology Journal, Journal Year: 2023, Volume and Issue: 21, P. 3339 - 3354

Published: Jan. 1, 2023

COVID-19 was declared a pandemic in March 2020, and since then, it has not stopped spreading like wildfire almost every corner of the world, despite many efforts made to stem its spread. SARS-CoV-2 one biggest genomes among RNA viruses presents unique characteristics that differentiate from other coronaviruses, making even more challenging find cure or vaccine is efficient enough. This work aims, using sequencing (RNA-Seq) data, evaluate whether expression specific human genes host can vary different grades disease severity determine molecular origins differences response infection patients. In addition quantifying gene expression, data coming RNA-Seq allow for discovery new transcripts, identification alternative splicing events, detection allele-specific post-transcriptional alterations. For this reason, we performed differential analysis on profiles patients, NCBI public repository, obtained lists all differentially expressed (DEGs) emerging 7 experimental conditions. We Gene Set Enrichment Analysis (GSEA) these possible correlations between DEGs known phenotypes. mainly focused out contrasts involving severe conditions infer any relation worsening clinical picture an over-representation genes. Based results, study indicates small group result up-regulated form disease. EXOSC5, MESD, REXO2, TRMT2A are present conditions, being good biomarkers candidates The use over-expressed genes, down-regulated, which have individual role each condition as biomarker, assist early diagnosis.

Language: Английский

Citations

7
ACE2 and a Traditional Chinese Medicine Formula NRICM101 Could Alleviate the Inflammation and Pathogenic Process of Acute Lung Injury DOI Creative Commons
Cheng‐Han Lin, Yi‐Ju Chen,

M. C. Lin

et al.

Medicina, Journal Year: 2023, Volume and Issue: 59(9), P. 1554 - 1554

Published: Aug. 26, 2023

COVID-19 is a highly transmittable respiratory illness caused by SARS-CoV-2, and acute lung injury (ALI) the major complication of COVID-19. The challenge in studying SARS-CoV-2 pathogenicity limited availability animal models. Therefore, it necessary to establish models that can reproduce multiple characteristics ALI study therapeutic applications. present established mouse model has features are similar syndrome investigate role ACE2 administration Chinese herbal prescription NRICM101 ALI. Mice with genetic modifications, including overexpression human (K18-hACE2 TG) absence (mACE2 KO), were intratracheally instillated hydrochloric acid. acid intratracheal instillation induced severe immune cell infiltration, cytokine storms, pulmonary disease mice. Compared K18-hACE2 TG mice, mACE2 KO mice exhibited dramatically increased levels inflammatory cytokines (IL-6 TNF-α) bronchoalveolar lavage fluid, histological evidence injury, dysregulation MAPK MMP activation. In could ameliorate progression acid-induced conclusion, provided an effective platform for researchers pathological mechanisms develop strategies ALI, COVID-19-related

Language: Английский

Citations

7
Novel SARS-CoV-2 inhibition properties of the anti-cancer Kang Guan Recipe herbal formula DOI
Wei‐Jan Wang, Hsuan-Ting Tang, Shi‐Chen Ou

et al.

Cancer Letters, Journal Year: 2024, Volume and Issue: 604, P. 217198 - 217198

Published: Aug. 27, 2024

Language: Английский

Citations

1
Taiwan Chingguan Yihau (NRICM101) prevents kainic acid-induced seizures in rats by modulating neuroinflammation and the glutamatergic system DOI Creative Commons
Chi‐Feng Hung, Wei‐Che Chiu,

Jia-Cih Chen

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 15, 2024

Abstract Taiwan Chingguan Yihau (NRICM101) is a Traditional Chinese medicine (TCM) formula used to treat coronavirus disease 2019; however, its impact on epilepsy has not been revealed. Therefore, the present study evaluated anti-epileptogenic effect of orally administered NRICM101 kainic acid (KA)-induced seizures in rats and investigated possible mechanisms action. Sprague‒Dawley were (300 mg/kg) by oral gavage for 7 consecutive days before receiving an intraperitoneal injection KA (15 mg/kg). considerably reduced seizure behavior electroencephalographic induced rats. also significantly decreased neuronal loss glutamate increase increased GLAST, GLT-1, GAD67, GDH GS levels cortex hippocampus KA-treated In addition, suppressed astrogliosis (as determined GFAP expression); neuroinflammatory signaling HMGB1, TLR-4, IL-1β, IL-1R, IL-6, p-JAK2, p-STAT3, TNF-α, TNFR1 p-IκB levels, cytosolic p65-NFκB levels); necroptosis p-RIPK3 p-MLKL levels) The effects similar those carbamazepine, well-recognized antiseizure drug. Furthermore, no toxic liver kidney observed NRICM101-treated results indicate that antiepileptogenic neuroprotective through suppression inflammatory cues (HMGB1/TLR4, Il-1β/IL-1R1, IL-6/p-JAK2/p-STAT3, TNF-α/TNFR1/NF-κB) pathways (TNF-α/TNFR1/RIP3/MLKL) associated with level regulation brain innocuous. Our findings highlight promising role management epilepsy.

Language: Английский

Citations

0
NRICM101 in combatting COVID-19 induced brain fog: Neuroprotective effects and neurovascular integrity preservation in hACE2 mice DOI Creative Commons
Cher-Chia Chang, Yea‐Hwey Wang, Jiin‐Cherng Yen

et al.

Journal of Traditional and Complementary Medicine, Journal Year: 2024, Volume and Issue: 15(1), P. 36 - 50

Published: July 3, 2024

Amidst growing concerns over COVID-19 aftereffects like fatigue and cognitive issues, NRICM101, a traditional Chinese medicine, has shown promise. Used by 2 million people globally, it notably reduces hospitalizations intubations in patients. To explore whether NRICM101 could combat brain fog, we tested on hACE2 transgenic mice administered the S1 protein of SARS-CoV-2, aiming to mitigate S1-induced issues measuring animal behaviors, immunohistochemistry (IHC) staining, next-generation sequencing (NGS) analysis. The study revealed that protein-administered displayed marked signs characterized reduced learning, memory, nesting abilities. However, treatment these animals ameliorated all functions. administration induced notable inflammation, leading death neurons (NeuN+) neural stem cells (DCX+) mice. This was accompanied heightened microglia activation (IBA1+/CD68+), increased cytokine production (IL1β, IL6), induction neutrophil extracellular traps (NET), inflammation (NLRP3, CD11b), platelet (CD31, vWF) complement (C3) activation, ultimately damaging neurovasculature disrupting blood-brain barrier (B.B.B.). Administration effectively alleviated pathological changes. In conclusion, potential prevent COVID-19-associated fog bolstering neurovascular integrity protecting cells. is achieved inhibition protein-induced which turn leads prevention damage subsequent neurons.

Language: Английский

Citations

0