Improving TMJ Diagnosis: A Deep Learning Approach for Detecting Mandibular Condyle Bone Changes DOI Creative Commons
Kader Azlağ Pekince, Adem Pekince,

Buse Yaren Kazangirler

et al.

Diagnostics, Journal Year: 2025, Volume and Issue: 15(8), P. 1022 - 1022

Published: April 17, 2025

Objectives: This paper evaluates the potential of using deep learning approaches for detection degenerative bone changes in mandibular condyle. The aim this study is to enable and diagnosis condyle degenerations, which are difficult observe diagnose on panoramic radiographs, methods. Methods: A total 3875 condylar images were obtained from radiographs. Condylar represented by flattening, osteophyte, erosion, two or more these observed labeled as "other". Due limited number containing osteophytes used. In first approach, erosion combined into "other" group, resulting three groups: normal, deformation ("deformation" encompasses together with osteophyte erosion). second completely excluded, other. utilizes a range advanced algorithms, including Dense Networks, Residual VGG Google pre-trained transfer techniques. Model performance was evaluated datasets different distributions, specifically 70:30 80:20 training-test splits. Results: GoogleNet architecture achieved highest accuracy. Specifically, split normal-flattening-deformation dataset Adamax optimizer, an accuracy 95.23% achieved. results demonstrate that CNN-based methods highly successful determining changes. Conclusions: demonstrates learning, particularly CNNs, accurate efficient TMJ-related approach could assist clinicians identifying patients requiring further intervention. Future research may involve cross-sectional imaging training right left condyles potentially increase success rate. has improve early changes, enabling timely referrals preventing disease progression.

Language: Английский

Bioactive scaffolds integrated with micro-precise spatiotemporal delivery and in vivo degradation tracking for complex tissue regeneration DOI Creative Commons

Hun Jin Jeong,

Alia Koch,

Soomin Park

et al.

Engineered Regeneration, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Synergistic effects of estrogen deficiency and articular disk derangement on condylar bone loss DOI

Kazuhiro Shibusaka,

Soichiro Negishi,

Nobuhiro Sakai

et al.

Journal of Oral Biosciences, Journal Year: 2025, Volume and Issue: unknown, P. 100616 - 100616

Published: Jan. 1, 2025

Language: Английский

Citations

0
Causal Links Between Bone Diseases and Temporomandibular Disorders DOI Creative Commons
Ling Zhang, Qinghua Zhang, Dong Zhao

et al.

International Dental Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Epidemiological observational studies have explored the link between bone joint-related diseases and temporomandibular disorders (TMD), but inconsistent conclusions emerged due to various limitations. This study aims investigate causal relationship TMD using Mendelian randomization (MR). We utilized a two-sample MR design, applying pooled genome-wide association (GWAS) data from six subtypes of joint TMD. Primary analysis was conducted inverse-variance weighted (IVW) method, complemented by median (WM), mode, MR-Egger regression assess relationships. Additionally, we performed reverse causality analyses applied sensitivity analyses, including MR-PRESSO, MR-Egger, Cochran's Q, leave-one-out methods evaluate result robustness, explore heterogeneity, identify potential biases. genetic prediction indicated that increase risk TMD, with specific odds ratios (OR) for ankylosing spondylitis (AS: OR 1.36, 95% CI: 1.04-1.77, P = .026), rheumatoid arthritis (RA: 1.08, 1.03-1.13, .001), osteoporosis (OR 1.0751, 1.0047-1.1505, .036). Conversely, revealed positive RA 1.12, 1.02-1.23, .018). showed no influence horizontal pleiotropy, MR-PRESSO detected outliers. The confirmed results' stability. findings demonstrate positive, AS, RA, osteoporosis. Moreover, patients exhibit an increased developing RA. Understanding these relationships aids in better diagnosis management its diseases, potentially guiding clinical interventions.

Language: Английский

Citations

0
Polydopamine-loaded microneedles for sequential tumor ablation and osteochondral regeneration DOI
Bin Lin, Yong Xu,

Bing Liang

et al.

Materials Letters, Journal Year: 2025, Volume and Issue: unknown, P. 138150 - 138150

Published: Jan. 1, 2025

Language: Английский

Citations

0
Betaine enhances SCAPs chondrogenic differentiation and promotes cartilage repair in TMJOA through WDR81 DOI Creative Commons
Meiyue Wang, Zhaoming Wu, Xiaoyu Zheng

et al.

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 7, 2025

The cartilage tissue regeneration mediated with mesenchymal stem cells (MSCs) is considered as a viable strategy for temporomandibular joint osteoarthritis (TMJOA). Betaine has been confirmed to modulate the multidirectional differentiation of MSCs, while its effect on chondrogenic Stem Cells from Apical Papilla (SCAPs) unknown. Here, we explored effects and underlying mechanisms betaine SCAPs. was added SCAPs induction. potential assessed using Alcian Blue staining, Sirius Red staining main markers. In vivo were evaluated by rat TMJOA model. RNA-sequencing biological analyses performed select target genes processes involved. mechanism acts further explored. Betain-treated demonstrated stronger in vitro promoted repair vivo. enhanced expression WDR81 during chondrogenesis. overexpression SCAPs, depletion inhibited differentiation. addition, both treatment reduced intracellular reactive oxygen species levels increased mitochondrial membrane promotes provided an effective candidate treatment. may serve drug through mitophagy.

Language: Английский

Citations

0
FSP1/S100A4-Expressing Stem/Progenitor Cells Are Essential for Temporomandibular Joint Growth and Homeostasis DOI

Ticha Tuwatnawanit,

Wilma Wessman,

Denisa Belisova

et al.

Journal of Dental Research, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 14, 2025

The temporomandibular joint (TMJ) is one of the most used joints in body. Defects and wear cartilage joint, condyle, fibrocartilage disc lie at heart many common TMJ disorders. During postnatal development, condyle acts as a growth center for mandible, with cells moving conveyor belt away from top they differentiate. superficial layers have been proposed to contain stem/progenitor populations allow maintain homeostasis. Here we focused on role fibroblast-specific protein 1 (FSP1; also known S100a4) key fibroblast marker condyle. Lineage tracing FSP1-Cre;R26RmTmG mice revealed that FSP1-expressing were restricted zone, giving rise all over time. overlapped other putative stem cell markers such Gli1 scleraxis. BrdU pulse chase experiments highlighted subset FSP1 was label retaining, suggesting labels novel population Destruction by conditional diphtheria toxin activity FSP1-Cre;R26RDTA resulted severe osteoarthritis loss structure. Lgr5-expressing layer shown create Wnt inhibitory niche. expression postnatally associated reduction canonical Importantly, constitutive activation Wnt/β catenin led downregulation progressive condylar hyaline due cells. These data demonstrate zone maintenance highlight importance regulating this population.

Language: Английский

Citations

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Akt2 inhibition alleviates temporomandibular joint osteoarthritis by preventing subchondral bone loss DOI Creative Commons
Shi‐Yang Feng, Miao Cao, Chenchen Gao

et al.

Arthritis Research & Therapy, Journal Year: 2025, Volume and Issue: 27(1)

Published: Feb. 27, 2025

This study aimed to investigate the role and mechanism of Akt2 pathway in different stages anterior disc displacement (ADD)-induced temporomandibular joint osteoarthritis (TMJOA). A rat model for TMJOA that simulates was established. For inhibit expression subchondral bone, rats were intravenously injected with adeno-associated virus carrying shRNA at a titer 1 × 1012 transducing units/mL 10 days before ADD or sham operations. The euthanized evaluated 8 weeks after surgery, as these time points represented early advanced stage ADD. Immunostaining performed examine location phosphorylated Microcomputed tomography, hematoxylin eosin staining, toluidine blue Western blotting, immunohistochemical immunofluorescence staining used elucidate pathological changes potential mechanisms underlying ADD-induced TMJOA. In TMJOA, rapid condylar bone loss occurred increased phosphorylation macrophages within week post-surgery. At abnormal remodeling degenerative cartilage observed. Inhibiting reduced resorption following surgery while improving morphology Additionally, inhibition alleviated degeneration characterized by decreased number apoptotic chondrocytes, matrix metalloproteinases, collagen type II tissue. is activated mainly during plays an important regulating remodeling. Inhibition could serve prophylactic treatment slow progression

Language: Английский

Citations

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TMJ Pericapsular Guided Injection with Visual-Inertial Odometry (Augmented/Mixed Reality): a Novel Pilot Clinical Approach for Joint Osteoarthrosis Drug Delivery. DOI Creative Commons
Marco Farronato,

Roberta Crispino,

Massimo Del Fabbro

et al.

Journal of Dentistry, Journal Year: 2025, Volume and Issue: unknown, P. 105659 - 105659

Published: March 1, 2025

Temporomandibular joint (TMJ) osteoarthrosis often requires pericapsular injections for symptom management. Image-guided techniques have demonstrated superior outcomes compared to traditional manual palpation. Augmented reality (AR) integrates digital images into the clinical scenario in real time and proved be an efficient surgical guide. This pilot study evaluated of AR-assisted versus palpation polynucleotides (PN) subjects with TMJ osteoarthrosis. 20 condyles (10 patients) bilateral were randomly assigned either test (N=10 - 5 patients; 53 years old SD 13; 4 female 6 male) or control group 58 15; male): AR-guided standard injection site localization following employed custom visual-inertial odometry (VIO) algorithm, using condyle's segmented precise estimation. Mouth opening, lateral movements, pain intensity assessed at baseline (T0), 2 weeks (T1), (T2), 12 (T3) post-injection. Statistical analysis included non-parametric Mann-Whitney between groups all assessed. showed improvements VAS scores mandibular kinematics over time. The significant reduction increased movement. There was no statistically difference two terms overall efficacy. procedure took approximately 120 seconds longer (mean 343.8 sec 211.2 sec). new technique efficacy improvement trends some parameters such as laterality, a reasonable increase timing practice. first application AR can considered guided injections, but further research is needed. offered making it promising digitally approach daily practice without need direct

Language: Английский

Citations

0
Advanced progress of adipose-derived stem cells-related biomaterials in maxillofacial regeneration DOI Creative Commons
Lijun Zhang,

Zihang Yu,

Shuchang Liu

et al.

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 5, 2025

The tissue injury in maxillofacial region affects patients' physical function and specific mental health. This decade, utilizing regenerative medicine to achieve regeneration has been proved a hopeful direction. Seed cells play vital role strategy. Among various kinds of stem that effectively regenerate the soft hard region, adipose-derived (ADSCs) have gained increasing interests researchers due their abundant sources, easy availability multi-differentiation potentials recent decades. Thus, this review focuses on advances ADSCs-based biomaterial from progress strategies perspective. It is structured as introducing properties ADSCs, biomaterials (polymers, ceramics metals) within ADSCs latest applications regeneration, including temporomandibular joint (TMJ), bone, periodontal tissue, tooth, nerve well cosmetic field. In order further facilitate therapies an emerging platform for medicine, also emphasized current challenges translating ADSC-based into clinical application dissussed solve these obstacles.

Language: Английский

Citations

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Temporomandibular Joint Degeneration Arises Spontaneously in STR/Ort Mice and is Prevented by Targeted Aggrecanase Inhibition DOI Creative Commons
Kazuhiro Ooi, Kazuhiro Yamamoto, Yutaka Kobayashi

et al.

Osteoarthritis and Cartilage Open, Journal Year: 2025, Volume and Issue: 7(2), P. 100599 - 100599

Published: March 12, 2025

Temporomandibular joint osteoarthritis (TMJ-OA) is painful and causes masticatory dysfunction, but current treatment limited to symptom relief due an incomplete appreciation of aetiology. Herein, we develop morphological histological methods for quantitative evaluation TMJ-OA severity examine whether STR/Ort mice, which are genetically predisposed spontaneous knee OA, exhibit protection against upon genetic gain-of-function modification aggrecanase-selective mutant tissue inhibitor metalloproteinase (TIMP)-3. We established changes in mandibular condylar head adapted from human criteria, developed verified the utility damage scoring OARSI system. Mutant TIMP3 containing extra alanine at N-Terminus ([-1A] TIMP-3 was overexpressed CBA mice. Morphological condyle TMJ cartilage degradation were evaluated quantified using micro-CT histology mice aged 10, 20 40 weeks. Whilst no evidence observed 10 weeks, bone erosion osteophyte formation appeared by with remarkable deformity resorption weeks STR/Ort, not parental strain. less severe week-old [-1A]TIMP-3 overexpressing compared wild-type Using our new mouse system have found that OA affects joints other than Genetic (TIMP)-3 also affords vivo chondroprotection this TMJ-OA.

Language: Английский

Citations

0