Discovery and Detection of Dry Eye Disease Protein Biomarkers Using Soft Contact Lens Tear Sampling DOI Open Access

Robert K. Roden,

Fangfang Jiang,

Alyssa A Nitz

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: May 26, 2023

Abstract Dry eye disease (DED) affects up to half of the world’s population and causes blur, pain, decreased quality life. Unfortunately, many people do not receive proper treatment due current challenges associated with DED diagnoses. To address this issue, point-of-care (PoC) clinical tear film diagnostics are beginning be used for rapid in-office testing. However, these also have significant obstacles, including sampling. We previously demonstrated how soft contact lenses (SCLs) can capture concentrate proteins on eye. Given low volume subjects, SCL sampling holds great potential improve PoC facilitate prompt patients. Here we show detection new established protein biomarkers using our method. Our analysis revealed associations between immune system, ferroptosis, salivary secretion, calcium binding proteins. identified correlations testing proteomics, emphasizing power importance translational medicine research. These experiments lay foundation both sampled biomarker discovery as well improved diagnostic patient care. Figure

Language: Английский

Potential New Target for Dry Eye Disease—Oxidative Stress DOI Creative Commons
Jinghua Bu, Yanbo Liu, Rongrong Zhang

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(4), P. 422 - 422

Published: March 29, 2024

Dry eye disease (DED) is a multifactorial condition affecting the ocular surface. It characterized by loss of tear film homeostasis and accompanied symptoms that may potentially result in damage to surface even vision loss. Unmodifiable risk factors for DED mainly include aging, hormonal changes, lifestyle issues such as reduced sleep duration, increased screen exposure, smoking, ethanol consumption. As its prevalence continues rise, has garnered considerable attention, prompting exploration potential new therapeutic targets. Recent studies have found when production ROS exceeds capacity antioxidant defense system on surface, oxidative stress ensues, leading cellular apoptosis further damage. These events can exacerbate inflammation responses, increasing levels promoting vicious cycle DED. Therefore, given central role reactive oxygen species DED, strategies involving antioxidants emerged novel approach treatment. This review aims enhance our understanding intricate relationship between thereby providing directions explore innovative approaches this complex disorder.

Language: Английский

Citations

22
GSK-3β-dependent Nrf2 antioxidant response modulates ferroptosis of lens epithelial cells in age-related cataract DOI

Dong-yue Ma,

Jinxia Liu, Ludi Wang

et al.

Free Radical Biology and Medicine, Journal Year: 2023, Volume and Issue: 204, P. 161 - 176

Published: May 6, 2023

Language: Английский

Citations

26
Oridonin ameliorates ocular surface inflammatory responses by inhibiting the NLRP3/caspase-1/GSDMD pyroptosis pathway in dry eye DOI
Xiaojing Li, Chen Chen, Ying Chen

et al.

Experimental Eye Research, Journal Year: 2024, Volume and Issue: 245, P. 109955 - 109955

Published: June 5, 2024

Language: Английский

Citations

9
Imaging assessment of conjunctival goblet cells in dry eye disease DOI Creative Commons
Y Liu, Zhengyu Duan, Jin Yuan

et al.

Clinical and Experimental Ophthalmology, Journal Year: 2024, Volume and Issue: 52(5), P. 576 - 588

Published: March 30, 2024

Abstract Dry eye disease (DED) is a widespread, multifactorial, and chronic disorder of the ocular surface with disruption tear film homeostasis as its core trait. Conjunctival goblet cells (CGCs) are specialised secretory found in conjunctival epithelium that participate formation by secreting mucin. Changes both structure function CGCs hallmarks DED, imaging assessment important for diagnosis, classification, severity evaluation DED. Existing methods include biopsy, impression cytology vivo confocal microscopy, which can be used to assess morphology, distribution, density CGCs. Recently, moxifloxacin‐based fluorescence microscopy has emerged novel technique enables efficient, non‐invasive This article presents comprehensive overview their alterations context well current assessment. Additionally, potential directions visual discussed.

Language: Английский

Citations

4
Development of Human Amniotic Epithelial Cell-derived Extracellular Vesicles as Cell-free Therapy for Dry Eye Disease DOI
Soojin Yi, Jeong‐Ho Kim,

Mi Ju Kim

et al.

The Ocular Surface, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 1, 2024

Language: Английский

Citations

4
Oxazole-Based Ferroptosis Inhibitors with Promising Properties to Treat Central Nervous System Diseases DOI Creative Commons
Camilla Scarpellini, Greta Klejborowska, Caroline Lanthier

et al.

Journal of Medicinal Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

Ferroptosis plays an important role in the occurrence and development of many diseases, including neurodegenerative diseases. Thus, ferroptosis inhibitors able to cross blood-brain barrier may have therapeutic potential. The best so far are lipophilic radical trapping antioxidants (RTAs) that block lipid peroxidation membranes. Several generations ferrostatins been synthesized, among which UAMC-3203 showed high potency animal models with improved properties compared ferrostatin-1. To further improve its pharmacokinetics properties, drug-likeness, permeability, we modified by decreasing size molecule reducing polarity replacing sulfonamide first amide groups subsequently isosteric oxazoles. Herein, present design, synthesis, biological evaluation a novel series oxazole RTAs potency, excellent oral bioavailability, concentrations brain tissue.

Language: Английский

Citations

0
Role of Chinese Medicine Monomers in Dry Eye Disease: Breaking the Vicious Cycle of Inflammation DOI Creative Commons

Zhuoyu Hu,

Xiangdong Chen, Qi Hu

et al.

Pharmacology Research & Perspectives, Journal Year: 2025, Volume and Issue: 13(2)

Published: Feb. 20, 2025

Dry eye disease (DED) is a chronically inflammatory ocular surface disorder of unknown pathogenesis. Anti-inflammatory medications, artificial tears, autologous serum, and LipiFlow have been shown to be highly beneficial in alleviating symptoms. Nevertheless, these interventions often provide only short-term results do not address the underlying problems disease. There growing evidence that risk DED associated with vicious cycle inflammation. This inflammation produced by interaction several factors, including tear film hyperosmolarity, instability, inflammation, apoptosis. Chinese medicine monomers, distinguished their multicomponent multitarget advantages, help treat modulating status, inhibiting responses, apoptosis, providing new way thinking management medicine.

Language: Английский

Citations

0
Oroxylin A alleviates pyroptosis and apoptosis in human corneal epithelial cells under hyperosmotic stress by activating the SIRT3-SOD2/HIF-1α pathway DOI
Xueqing Liu, Qiang Xu, Nan Jiang

et al.

Experimental Eye Research, Journal Year: 2025, Volume and Issue: 255, P. 110345 - 110345

Published: March 15, 2025

Language: Английский

Citations

0
Parainflammation in the Ocular System: Considerations on the Underlying Mechanisms and Treatment of Dry Eye Disease DOI Creative Commons
Stefano Barabino, Pasquale Aragona, Christophe Baudouin

et al.

Ophthalmology and Therapy, Journal Year: 2025, Volume and Issue: unknown

Published: March 29, 2025

The transition from regulated to dysregulated parainflammation is a new concept that needs be elucidated clarify the pathogenesis of dry eye disease (DED). This review summarizes recent evidence about mechanisms could lead parainflammation, proposing hypothesis correlate this process with progression chronic inflammation. A group European experts on DED participated in roundtable discuss role most common ocular diseases regard DED. Starting contents, narrative was conducted through PubMed search based main topics discussed, namely: dysfunctional tear film lipid and mucin alterations, cortisol. Parainflammation involved different pathologies characterized by involvement immune system complement factors. In DED, continuous persistent insults are responsible for qualitative quantitative alteration components film. addition, other contributing factors have recently been described, such as reduction cortisol synthesis corneal epithelial cells. altered condition leads excessive macrophage activity, releasing cytokines adhesion molecules, losing tissue homeostasis, possibly progressing Literature supports crucial its usefulness improving diagnosis treatment At same time, further investigations necessary better define functional including surface than

Language: Английский

Citations

0
Multifunctional Cerium Oxide Nanozyme for Synergistic Dry Eye Disease Therapy DOI

Haoyu Zou,

Yueze Hong,

Baoqi Xu

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(27), P. 34757 - 34771

Published: July 1, 2024

Dry eye disease (DED) is a chronic multifactorial ocular surface mainly caused by the instability of tear film, characterized series discomforts and even visual disorders. Oxidative stress has been recognized as an upstream factor in DED development. Diquafosol sodium (DQS) agonist P2Y2 receptor to restore integrity/stability film. With ability alternate between Ce3+ Ce4+, cerium oxide nanozymes could scavenge overexpressed reactive oxygen species (ROS). Hence, DQS-loaded nanozyme was designed boost synergistic treatment DED. Cerium with branched polyethylenimine-graft-poly(ethylene glycol) nucleating agent dispersant fabricated followed DQS immobilization via dynamic phenylborate ester bond, obtaining (defined Ce@PBD). Because mimic cascade processes superoxide dismutase catalase, Ce@PBD excessive accumulated ROS, showing strong antioxidant anti-inflammatory properties. Meanwhile, receptors conjunctival cells be stimulated Ce@PBD, which can relieve incompleteness The animal experiments demonstrated that significantly restored defect corneal epithelium increased number goblet cells, promotion secretion, best among commercial ophthalmic solutions.

Language: Английский

Citations

2