Nanobiotechnology Reports, Journal Year: 2024, Volume and Issue: 19(6), P. 1030 - 1037
Published: Dec. 1, 2024
Language: Английский
Frontiers in Aging Neuroscience, Journal Year: 2023, Volume and Issue: 15
Published: Aug. 23, 2023
Neurodegenerative diseases are a large class of neurological disorders characterized by progressive dysfunction and death neurones. Examples include Alzheimer's disease, Parkinson's frontotemporal dementia, amyotrophic lateral sclerosis. Aging is the primary risk factor for neurodegeneration; individuals over 65 more likely to suffer from neurodegenerative with prevalence increasing age. As population ages, social economic burden caused these will increase. Therefore, new therapies that address both aging neurodegeneration imperative. Ketogenic diets (KDs) low carbohydrate, high-fat developed initially as an alternative treatment epilepsy. The classic ketogenic diet provides energy via long-chain fatty acids (LCFAs); naturally occurring medium chain (MCFAs), on other hand, main components medium-chain triglyceride (MCT) diet. MCT-based efficient at generating ketone bodies used secondary source neurones astrocytes. However, levels alone do not closely correlate improved clinical symptoms. Recent findings suggest mode action MCFAs, e.g., improving mitochondrial biogenesis glutamate receptor inhibition. MCFAs have been linked disease their effects metabolism. Through multiple disease-related pathways, emerging compounds notable potential promote healthy ameliorate neurodegeneration. shown stimulate autophagy restore function, which found be disrupted in This review aims provide insight into metabolic benefits aging. We discuss use combat dysregulation function context "normal" aging, sclerosis disease.
Language: Английский
Citations
18
Biochemistry (Moscow), Journal Year: 2024, Volume and Issue: 89(2), P. 257 - 268
Published: Feb. 1, 2024
Language: Английский
Citations
4
International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2152 - 2152
Published: Feb. 27, 2025
Mitochondria are involved in a wide array of critical cellular processes from energy production to cell death. The morphology (size and shape) mitochondrial compartments is highly responsive both intracellular extracellular conditions, making these organelles dynamic. Nutrient levels stressors inside outside the inform balance fission fusion recycling components known as mitophagy. study its implications human disease microbial engineering have gained significant attention over past decade. yeast Saccharomyces cerevisiae offers valuable model system for studying mitochondria due ability survive without respiring, genetic tractability, high degree similarity across eukaryotic species. Here, we review how interplay between fission, fusion, biogenesis, mitophagy regulates dynamic nature networks mammalian systems with an emphasis on organism. Additionally, examine crucial role inter-organelle interactions, particularly endoplasmic reticulum, regulating dynamics. dysregulation any gives rise abnormal morphologies, which serve distinguishing features numerous diseases, including Parkinson's disease, Alzheimer's cancer. Notably, models contributed revealing underlying mechanisms driving states. In addition furthering our understanding pathologic processes, aberrant morphologies increasing interest seemingly distant field metabolic engineering, following discovery that compartmentalization certain biosynthetic pathways within can significantly improve chemical production. this review, utility organism healthy states, explore nascent discuss methods available quantification classification key morphologies.
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(2), P. 1107 - 1107
Published: Jan. 16, 2024
Oxidative stress is involved in a wide range of age-related diseases. A critical role has been proposed for mitochondrial oxidative initiating or promoting these pathologies and the potential mitochondria-targeted antioxidants to fight them, making their search testing very urgent task. In this study, SkQ1, SkQ3 MitoQ were examined as they affected isolated rat liver mitochondria yeast cells, comparing with clinically tested SkQ1 MitoQ. At low concentrations, all three substances stimulated oxidation respiratory substrates state 4 respiration (no ADP addition); at higher inhibited ADP-triggered 3 uncoupled state, depolarized inner membrane, contributed opening mPTP (mitochondrial permeability transition pore), did not specifically affect ATP synthase, had pronounced antioxidant effect. was most active antioxidant, possessing, unlike MitoQ, prooxidant activity increasing concentrations. reduced prooxidant-induced intracellular cell death prevented reversed fragmentation, being efficient. These data allow us consider promising therapeutic agent mitigate associated stress.
Language: Английский
Citations
3
Biochemistry (Moscow), Journal Year: 2024, Volume and Issue: 89(2), P. 212 - 222
Published: Feb. 1, 2024
Language: Английский
Citations
2
International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(12), P. 9791 - 9791
Published: June 6, 2023
Alzheimer’s disease (AD) is an age-related, multifaceted neurological disorder associated with accumulation of aggregated proteins (amyloid Aβ and hyperphosphorylated tau), loss synapses neurons, alterations in microglia. AD was recognized by the World Health Organization as a global public health priority. The pursuit better understanding forced researchers to pay attention well-defined single-celled yeasts. Yeasts, despite obvious limitations application neuroscience, show high preservation basic biological processes all eukaryotic organisms offer great advantages over other models due simplicity, growth rates on low-cost substrates, relatively simple genetic manipulations, large knowledge base data collections, availability unprecedented amount genomic proteomic toolboxes high-throughput screening techniques, inaccessible higher organisms. Research reviewed above clearly indicates that yeast models, together other, more including animal C. elegans Drosophila, significantly contributed tau biology. These allowed throughput factors drugs interfere oligomerization, aggregation toxicity, hyperphosphorylation. In future, will remain relevant, focus creating novel systems facilitate identification earliest biomarkers among different cellular networks order achieve main goal—to develop new promising therapeutic strategies treat or prevent disease.
Language: Английский
Citations
4
Биохимия, Journal Year: 2024, Volume and Issue: 89(2), P. 279 - 292
Published: Aug. 13, 2024
This paper presents new structural data about mitochondria using correlative light and electron microscopy cryo-electron tomography (cryo-ET). These state-of-the-art biology methods allow the study of biological objects at nanometer scales in natural conditions. The non-invasiveness these makes them comparable to observing animals their environment on a safari. highlights two areas research that can only be accomplished methods. visualized location Aβ42 amyloid aggregates relation test hypothesis for development mitochondrial dysfunction Alzheimer’s disease. results showed do not interact with mitochondria, although some are closely located. Therefore, demonstrated is directly influenced by structure. source should investigated other processes. Second unique area presented this work high-resolution visualization membranes proteins them. analysis cryo-ET reveals toroidal holes lamellar structures cardiac cristae, where ATP synthases proposes mechanism sorting clustering protein complexes membrane based topology. According mechanism, position oxidative phosohorylation system determined its curvature. High-resolution expands complements existing ideas functional organization mitochondria. it possible previously inaccessible interactions each vivo.
Language: Английский
Citations
0
Биохимия, Journal Year: 2024, Volume and Issue: 89(2), P. 228 - 240
Published: Aug. 13, 2024
Quinone derivatives of triphenylphosphonium have proven themselves to be effective geroprotectors and antioxidants that prevent the oxidation cell components with participation active free radicals – peroxide (RO2•), alkoxy (RO•), alkyl (R•), as well reactive oxygen species (superoxide anion, singlet oxygen). The most studied representatives are plastoquinone (SkQ1) ubiquinone (MitoQ), which in addition antioxidant properties also had a strong antibacterial effect. Other quinone based on decyltriphenylphosphonium (SkQ3, SkQT, SkQThy) been less their activity remains undiscovered. In this work, we investigated decyltriphenylphosphonium. We shown that, case SkQ1, they against various gram-positive bacteria, while not so gram-negative bacteria. This is associated recognition by main multidrug resistance pump bacteria AcrAB-TolC. Moreover, SkQ1 itself, there dependence its action number bacterial cells molar amount antibiotic distinguishes it from conventional antibiotics. At same time, cytotoxic effect mammalian observed higher concentrations, suggests presence protective caused separation process energy generation transport membrane. Another possible explanation could different composition multidrug-resistant (MDR) pumps. difference effects may due expression profiles MDR pumps types.
Language: Английский
Citations
0
Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 557 - 567
Published: Oct. 4, 2024
Language: Английский
Citations
0
Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: Dec. 3, 2024
Abstract Mitochondrial morphology is an important parameter of cellular fitness. Although many approaches are available for assessing mitochondrial in mammalian cells, only a few technically demanding and laborious methods yeast cells. A robust, fully automated user-friendly approach that would allow (1) segmentation tubular spherical mitochondria the Saccharomyces cerevisiae from conventional wide-field fluorescence images (2) quantitative assessment lacking. To address this, we compared Global thresholding with deep learning MitoSegNet segmentation, which retrained on The model outperformed segmentation. We applied it to segment strain lacking MMI1/TMA19 gene encoding ortholog human TCTP protein. Next, performed evaluation segmented by analyses ImageJ/Fiji MitoA analysis toolbox. By monitoring wide range morphological parameters, described novel phenotype mmi1Δ after its exposure oxidative stress wild-type strain. model, all macros run analyses, as well detailed procedure now at https://github.com/LMCF-IMG/Morphology_Yeast_Mitochondria .
Language: Английский
Citations
0