Adipocyte-targeted delivery of rosiglitazone with localized photothermal therapy for the treatment of diet-induced obesity in mice

Acta Biomaterialia, Journal Year: 2024, Volume and Issue: 181, P. 317 - 332

Published: April 19, 2024

Language: Английский

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The Crosstalk between Gut Microbiota and White Adipose Tissue Mitochondria in Obesity

Nutrients, Journal Year: 2023, Volume and Issue: 15(7), P. 1723 - 1723

Published: March 31, 2023

Adipose tissue (AT) dysregulation is a key process in the pathophysiology of obesity and its cardiometabolic complications, but even if growing body evidence has been collected over recent decades, underlying molecular basis adiposopathy remains to be fully understood. In this context, mitochondria, intracellular organelles that orchestrate energy production undergo highly dynamic adaptive changes response changing environments, have emerged as crucial regulators both white (WAT) brown adipose (BAT) metabolism function. Given gut microbiota metabolites are able regulate host metabolism, adipogenesis, WAT inflammation, thermogenesis, we hypothesize their frequently observed could affect AT by exerting direct indirect effects on mitochondria. By collecting revising current connections between mitochondria obesity, gained insights into biology hitherto largely unexplored crosstalk, tracing how may mitochondrial

Language: Английский

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Functional roles of pantothenic acid, riboflavin, thiamine, and choline in adipocyte browning in chemically induced human brown adipocytes

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Aug. 6, 2024

Brown fat is a therapeutic target for the treatment of obesity-associated metabolic diseases. However, nutritional intervention strategies increasing mass and activity human brown adipocytes have not yet been established. To identify vitamins required adipogenesis adipocyte browning, chemical compound-induced (ciBAs) were converted from dermal fibroblasts under serum-free vitamin-free conditions. Choline was found to be essential adipogenesis. Additional with pantothenic acid (PA) provided choline-induced immature browning properties maturation, including uncoupling protein 1 (UCP1) expression, lipolysis, mitochondrial respiration. high PA concentrations attenuated these effects along decreased glycolysis. Transcriptome analysis showed that low concentration activated genes, futile creatine cycle-related thermogenic which reversed by concentration. Riboflavin suppressed gene expression increased implying pathway different PA. Thiamine slightly genes In summary, our results suggest specific B choline are uniquely involved in regulation via cellular energy metabolism concentration-dependent manner.

Language: Английский

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Metformin delays the decline in thermogenic function of brown adipose tissue in a mouse model of Hutchinson-Gilford progeria syndrome

Experimental Gerontology, Journal Year: 2025, Volume and Issue: 201, P. 112702 - 112702

Published: Feb. 6, 2025

Brown adipose tissue (BAT) is the primary site for non-shivering thermogenesis in body and plays a crucial role maintaining core temperature. However, its function gradually declines with age. To mitigate age-related decline BAT thermogenic capacity, we treated progeroid mice metformin to investigate potential mechanisms by which can slow reduction function. We found that mice, after receiving treatment, showed significant improvement senescent state of brown adipocytes through activation SIRT1, effectively reduced mitochondrial oxidative stress. Additionally, slowed UCP1 expression levels tissue, thereby capacity mice. Moreover, inflammatory responses around cells, further improving overall tissue. These findings suggest down aging process targeting enhancing capacity.

Language: Английский

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Identification of key LncRNAs and mRNAs associated with intramuscular fat in pig via WGCNA

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: March 11, 2025

Intramuscular fat (IMF) not only directly affects the tenderness, juiciness, and overall flavour of meat but also plays a significant role in influencing consumer preferences for pork. Therefore, exploring key biomarkers that influence IMF deposition is highly important breeding high-quality typical quantitative trait regulated by interaction multiple coding noncoding RNAs. Traditional differential analysis methods typically focus on individual genes, making it difficult to identify genes their underlying mechanisms accurately. Weighted gene coexpression network (WGCNA) an efficient accurate method identifying characterizing pathways associated with complex traits. aim this study was construct mRNA‒lncRNA related using WGCNA explore potential candidate pigs. Full-length transcriptome sequencing performed 31 220-day-old Jiangquan black pigs raised same environment, expression matrix comprising 25,609 constructed. Nine modules were identified through WGCNA, number these ranging from 33 3648. The magenta module (corr = 0.7, P < 0.01) turquoise -0.77, significantly deposition. Hub each basis screening criteria GS > 0.4 MM 0.8. Combined enrichment protein‒protein (PPI) analysis, 18 mRNAs potentially selected: CRKL, CBL, PDGFRB, DOCK1, YWHAH, HSP90AB1, LOC100524873, NDUFA1, NDUFA11, NDUFA12, NDUFA2, NDUFAB1, NDUFB10, NDUFB3, NDUFB7, NDUFS5, NDUFS6, UQCR10. To regulatory lncRNAs process deposition, we constructed lncRNA‒mRNA‒pathway relationships between mRNAs, as well results Kyoto Encyclopedia Genes Genomes (KEGG) analysis. This includes four (TGOLN2, LOC100521518, LOC100524915, LOC100622481) predicts which regulate Through PPI involved identified, lncRNA preliminarily explored. Our findings provide new insights into pig lay foundation further exploration molecular

Language: Английский

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Different action of glucocorticoid receptor in adipose tissue remodelling to modulate energy homeostasis by chronic restraint stress

Lipids in Health and Disease, Journal Year: 2025, Volume and Issue: 24(1)

Published: March 27, 2025

Abstract Background Chronic stress in daily life is a well-known trigger for various health issues. Despite advancements obesity research, the mechanisms governing lipid metabolism adipose tissue during cachexia remain poorly understood. Methods A chronic restraint (CRS) model was used to induce significant physiological and psychological mice. Mice were subjected 6 h of 50 mL plastic tubes seven consecutive days. fasting control group included comparison. Post-stress assessments behavioural tests, glucose insulin tolerance tests indirect calorimetry. Blood samples collected mRNA protein analyses. Results CRS induced changes mice, including depression-like behaviours, weight loss reduced sensitivity. Notably, caused extensive remodelling. White (WAT) underwent ‘browning’ accompanied by an increase expression thermogenic proteins. This counteracted stress-induced ‘whitening’ brown (BAT), which exhibited impaired thermogenesis functionality, thereby maintaining energy balance systematically. The glucocorticoid receptor (GR) plays crucial role regulation these changes. GR levels inversely correlated BAT WAT, but aligned with patterns proteins across tissues. These findings suggest that under metabolic stress, mediates tissue-specific responses tissues, driving functional phenotypic transitions WAT maintain homeostasis. Conclusions study provides novel insights into contrasting phenotypes emaciation highlights critical GRs remodelling its potential as therapeutic target. Addressing GR-mediated tissues may help alleviate dysfunction promote browning, enhancing resistance.

Language: Английский

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Thermogenesis and Energy Metabolism in Brown Adipose Tissue in Animals Experiencing Cold Stress

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3233 - 3233

Published: March 31, 2025

Cold exposure is a regulatory biological functions in animals. The interaction of thermogenesis and energy metabolism brown adipose tissue (BAT) important for metabolic regulation cold stress. Brown adipocytes (BAs) produce uncoupling protein 1 (UCP1) mitochondria, activating non-shivering (NST) by fuel combustion from ATP production response to stimuli. To elucidate the mechanisms underlying BAT under stress, we explored how triggers activation regulates overall metabolism. First, briefly outline precursor composition function BA. Second, explore roles cAMP- kinase A (PKA) adenosine monophosphate-activated (AMPK) signaling pathways BA during Then, analyze mechanism which mitochondria homeostasis balance This research reveals potential therapeutic targets, such as PKA, AMPK, UCP1 PGC-1α, can be used develop innovative strategies treating diseases. Furthermore, it provides theoretical support optimizing stress strategies, including pharmacological genetic modulation thermogenic pathways, improve livestock.

Language: Английский

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Gene expression and metabolite levels converge in the thermogenic spadix of skunk cabbage

PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: 195(2), P. 1561 - 1585

Published: Feb. 6, 2024

The inflorescence (spadix) of skunk cabbage (Symplocarpus renifolius) is strongly thermogenic and can regulate its temperature at around 23 °C even when the ambient drops below freezing. To elucidate mechanisms underlying developmentally controlled thermogenesis thermoregulation in cabbage, we conducted a comprehensive transcriptome metabolome analysis across 3 developmental stages spadix development. Our RNA-seq revealed distinct groups expressed genes, with selenium-binding protein 1/methanethiol oxidase (SBP1/MTO) exhibiting highest levels florets. Notably, expression alternative (AOX) was consistently high from prethermogenic stage through Metabolome showed that alterations nucleotide correspond tissue-specific evident by substantial increase AMP study also reveals hydrogen sulfide, product SBP1/MTO, inhibits cytochrome c (COX)-mediated mitochondrial respiration, while AOX-mediated respiration remains relatively unaffected. Specifically, lower temperatures, inhibitory effect sulfide on COX-mediated increases, promoting shift toward dominance respiration. Finally, despite differential regulation genes metabolites throughout development, observed convergence gene metabolite accumulation patterns during thermogenesis. This synchrony may play key role regulated Moreover, such provide solid molecular basis for cabbage.

Language: Английский

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Effect of Different Fatty Acid Types on Mitochondrial Dysfunction Associated With Brown and Beige Adipose Tissue

Nutrition Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

Abstract Adipose tissue serves as a dynamic endocrine organ that is pivotal in metabolic regulation. Augmenting mitochondrial activity within this holds promise combating obesity. Mitochondrial function intricately modulated by diverse fatty acid compositions. This comprehensive review aimed to elucidate the molecular mechanisms underlying dysfunction induced various profiles. While saturated acids (SFAs) pose threat integrity, polyunsaturated (PUFAs), notably n-3, mitigate SFA-induced damage, concurrently regulating thermogenic gene expression. With regard monounsaturated (MUFAs), their impact on adipose remains relatively unexplored. Although human studies are imperative for insights, prioritizing consumption of n-3 and MUFAs has emerged strategic approach, potentially enhancing biogenesis pathways. synthesis underscores critical need further investigation differential effects types mitochondria, offering potential avenues obesity intervention.

Language: Английский

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NAD replenishment restores mitochondrial function and thermogenesis in the brown adipose tissue of mice with obesity

The Journal of Physiology, Journal Year: 2025, Volume and Issue: unknown

Published: May 5, 2025

Abstract Brown adipose tissue (BAT) is a highly specialized thermogenic and plays critical role in controlling energy expenditure metabolic homeostasis. BAT dysfunction associated with body weight gain disorders mice models. Here, we investigated the influence of NAD‐biosynthesis pathway control metabolism function. Using wide multi‐omics phenotypic panel isogenic strains BXD mice, found that closely gain, adiposity, insulin resistance, oxygen consumption, exercise capacity mitochondrial metabolism. Interestingly, high‐fat diet (HFD) treatment disrupted pathway, reducing Nampt NMNat3 protein contents inducing severe mice. Finally, oral nicotinamide riboside (NR), an NAD + precursor, significantly boosted levels preserved morphofunctional mitochondria aspects mitochondria, thermogenesis HFD‐fed These data point to synthesis as promising therapeutic adjuvant target management. image Key points Obesity impairs biosynthesis leading reduced capacity. enzymes are crucial for function BAT. Nicotinamide increases expression key proteins ‐related BAT, optimizing adaptive

Language: Английский

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