Exploring solute binding proteins in Pseudomonas aeruginosa that bind to γ‐aminobutyrate and 5‐aminovalerate and their role in activating sensor kinases DOI Creative Commons
Jean Paul Cerna‐Vargas, Tino Krell

MicrobiologyOpen, Journal Year: 2024, Volume and Issue: 13(3)

Published: May 23, 2024

The standard method of receptor activation involves the binding signals or signal-loaded solute proteins (SBPs) to sensor domains. Many histidine kinases (SHKs), which are activated by SBP binding, encoded adjacent their corresponding sbp gene. We examined three SBPs Pseudomonas aeruginosa PAO1, near genes for AgtS (PA0600) and AruS (PA4982) SHKs, determine how common this arrangement is. Ligand screening microcalorimetric studies revealed that PA0602 PA4985 preferentially bind GABA (KD = 2.3 0.58 μM, respectively), followed 5-aminovalerate 30 1.6 respectively) ethanoldiamine respectively). In contrast, AgtB (PA0604) exclusively recognizes 5-aminovaleric acid 2.9 μM). However, titrations did not show any between domain PA0602, regardless presence ligands. Similarly, bacterial two-hybrid assays demonstrate an interaction domain. Therefore, shk located nearby always functionally linked. previously identified PA0222 as a GABA-specific SBP. may be linked GABA's role trigger P. virulence.

Language: Английский

Sensing preferences for prokaryotic solute binding protein families DOI Creative Commons
Jean Paul Cerna‐Vargas, Beatriz Sánchez‐Romera, Miguel A. Matilla

et al.

Microbial Biotechnology, Journal Year: 2023, Volume and Issue: 16(9), P. 1823 - 1833

Published: Aug. 7, 2023

Solute binding proteins (SBPs) are of central physiological relevance for prokaryotes. These present substrates to transporters, but they also stimulate different signal transduction receptors. SBPs form a superfamily at least 33 protein Pfam families. To assess possible links between SBP sequence and the ligand recognized, we have inspected manually all three-dimensional structures deposited in data bank retrieved 748 prokaryotic that been solved complex with bound ligand. were classified into 26 The analysis ligands recognized revealed most families possess preference compound class. There three each bind preferentially saccharides amino acids. In addition, identified purines, quaternary amines, iron iron-chelating compounds, oxoanions, bivalent metal ions or phosphates. Phylogenetic analyses suggest convergent evolutionary events lead same functional link chemotaxis uptake is reflected similarities by chemoreceptors. Associating profiles will be help design experimental strategies aimed identification uncharacterized SBPs.

Language: Английский

Citations

10
Exploring solute binding proteins in Pseudomonas aeruginosa that bind to γ‐aminobutyrate and 5‐aminovalerate and their role in activating sensor kinases DOI Creative Commons
Jean Paul Cerna‐Vargas, Tino Krell

MicrobiologyOpen, Journal Year: 2024, Volume and Issue: 13(3)

Published: May 23, 2024

The standard method of receptor activation involves the binding signals or signal-loaded solute proteins (SBPs) to sensor domains. Many histidine kinases (SHKs), which are activated by SBP binding, encoded adjacent their corresponding sbp gene. We examined three SBPs Pseudomonas aeruginosa PAO1, near genes for AgtS (PA0600) and AruS (PA4982) SHKs, determine how common this arrangement is. Ligand screening microcalorimetric studies revealed that PA0602 PA4985 preferentially bind GABA (KD = 2.3 0.58 μM, respectively), followed 5-aminovalerate 30 1.6 respectively) ethanoldiamine respectively). In contrast, AgtB (PA0604) exclusively recognizes 5-aminovaleric acid 2.9 μM). However, titrations did not show any between domain PA0602, regardless presence ligands. Similarly, bacterial two-hybrid assays demonstrate an interaction domain. Therefore, shk located nearby always functionally linked. previously identified PA0222 as a GABA-specific SBP. may be linked GABA's role trigger P. virulence.

Language: Английский

Citations

1