Frontiers in Systems Biology,
Journal Year:
2024,
Volume and Issue:
4
Published: June 17, 2024
The
COVID-19
pandemic
has
tested
the
technical,
scientific,
and
industrial
resources
of
all
countries
worldwide.
Faced
with
absence
pharmacological
strategies
against
disease,
an
effective
plan
for
vaccinating
SARS-CoV-2
been
essential.
Due
to
lack
production
means
necessary
infrastructure,
only
a
few
nations
could
adequately
confront
this
pathogen
production,
storage,
distribution
scheme
in
place.
This
disease
become
endemic
many
countries,
especially
those
that
are
developing,
thus
necessitating
solutions
tailored
their
reality.
In
paper,
we
propose
silico
method
guide
design
towards
thermally
stable,
universal,
efficient,
safe
vaccine
candidate
using
bioinformatics,
immunoinformatics,
molecular
modeling
approaches
selection
antigens
higher
immunogenic
potential,
incorporating
them
into
surface
M13
phage.
Our
work
focused
on
phagemid
display
as
peptide
array
neutralizing
antibodies
(PdPANA).
alternative
approach
might
be
useful
during
development
process,
since
it
bring
improvements
terms
cost-effectiveness
durability,
ease
under
less
stringent
thermal
conditions
compared
existing
methods.
results
suggest
heavily
glycosylated
region
Spike
protein
(aa
344–583),
from
its
inter-glycosylated
regions,
antigenic
peptides
can
obtained
used
system.
PdPANA,
our
proposed
overcome
classic
shortcoming
posed
by
phage-display
technique
(i.e.,
time-consuming
task
vitro
screening
through
great
sized
libraries
non-useful
recombinant
proteins)
obtain
most
ideal
proteins
purposes.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(15), P. 8148 - 8148
Published: July 26, 2024
Bacteriophages
are
emerging
as
a
promising
alternative
in
combating
antibiotic-resistant
bacteria
amidst
the
escalating
global
antimicrobial
resistance
crisis.
Recently,
there
has
been
notable
resurgence
of
interest
phages,
prompting
extensive
research
into
their
therapeutic
potential.
Beyond
conventional
microbiology
and
virology
techniques,
such
genomics
proteomics,
novel
phenotypic
chemical
characterization
methods
being
explored.
Among
these,
is
growing
vibrational
spectroscopy,
especially
advanced
modalities
surface-enhanced
Raman
spectroscopy
(SERS),
tip-enhanced
(TERS),
atomic
force
microscopy-infrared
(AFM-IR),
which
offer
improved
sensitivity
spatial
resolution.
This
review
explores
spectrum
uses
for
bacteriophages,
including
its
role
diagnostics,
biosensing,
phage
detection,
assistance
phage-based
therapy,
advancing
basic
research.
Life,
Journal Year:
2023,
Volume and Issue:
13(2), P. 436 - 436
Published: Feb. 3, 2023
Antimicrobial
resistance
is
rising
as
a
major
global
public
health
threat
and
antibiotic
genes
are
widely
spread
among
species,
including
human
oral
pathogens,
e.g.,
Aggregatibacter
actinomycetemcomitans.
This
Gram-negative,
capnophilic,
facultative
anaerobe
well
recognized
causative
agent
leading
to
periodontal
diseases,
seriously
systemic
infections
endocarditis.
A.
actinomycetemcomitans
has
also
evolved
mechanisms
against
complement-mediated
phagocytosis
resiliently
survives
in
serum-rich
vivo
environments,
i.e.,
inflamed
pockets
blood
circulations.
bacterium,
however,
demonstrated
increasing
sensitivity
serum,
when
being
infected
by
pseudolysogenic
bacteriophage
S1249,
which
switched
the
lytic
state
response
serum.
Concomitantly,
pyruvate
dehydrogenase
complex
(PDHc),
composed
of
multiple
copies
three
enzymes
(E1,
E2,
E3)
oxidatively
decarboxylates
acetyl-CoA
available
for
tricarboxylic
acid
(TCA)
cycle,
was
found
up-regulated
10-fold
bacterial
lysogen
after
serum
exposure.
The
data
clearly
indicated
that
certain
components
induced
phage
virion
replication
egress,
resulting
lysis.
Phage
manipulation
ATP
production
through
regulation
PDHc,
gatekeeper
linking
glycolysis
TCA
cycle
aerobic
respiration,
suggests
more
efficient
energy
delivery
system
required
progeny
release
this
environment.
Insights
into
fitness
mimic
condition
will
provide
alternative
strategies
control
infection,
addition
antibiotics.
Frontiers in Systems Biology,
Journal Year:
2024,
Volume and Issue:
4
Published: June 17, 2024
The
COVID-19
pandemic
has
tested
the
technical,
scientific,
and
industrial
resources
of
all
countries
worldwide.
Faced
with
absence
pharmacological
strategies
against
disease,
an
effective
plan
for
vaccinating
SARS-CoV-2
been
essential.
Due
to
lack
production
means
necessary
infrastructure,
only
a
few
nations
could
adequately
confront
this
pathogen
production,
storage,
distribution
scheme
in
place.
This
disease
become
endemic
many
countries,
especially
those
that
are
developing,
thus
necessitating
solutions
tailored
their
reality.
In
paper,
we
propose
silico
method
guide
design
towards
thermally
stable,
universal,
efficient,
safe
vaccine
candidate
using
bioinformatics,
immunoinformatics,
molecular
modeling
approaches
selection
antigens
higher
immunogenic
potential,
incorporating
them
into
surface
M13
phage.
Our
work
focused
on
phagemid
display
as
peptide
array
neutralizing
antibodies
(PdPANA).
alternative
approach
might
be
useful
during
development
process,
since
it
bring
improvements
terms
cost-effectiveness
durability,
ease
under
less
stringent
thermal
conditions
compared
existing
methods.
results
suggest
heavily
glycosylated
region
Spike
protein
(aa
344–583),
from
its
inter-glycosylated
regions,
antigenic
peptides
can
obtained
used
system.
PdPANA,
our
proposed
overcome
classic
shortcoming
posed
by
phage-display
technique
(i.e.,
time-consuming
task
vitro
screening
through
great
sized
libraries
non-useful
recombinant
proteins)
obtain
most
ideal
proteins
purposes.
