Journal of Current Ophthalmology,
Journal Year:
2023,
Volume and Issue:
35(2), P. 170 - 176
Published: April 1, 2023
Purpose:
To
obtain
a
reactive
and
specific
antibody
against
truncated
matrix
metalloproteinase
9
(MMP-9),
that
has
reactivity
toward
the
native
protein.
Precision,
accuracy,
specificity,
sensitivity
were
evaluated
using
point-of-care
test.
Methods:
An
in
silico
study
was
used
to
confirm
anti
peptide
MMP-9
is
MMP-9.
After
an
titer
assessment,
purification,
characterization,
assessed.
The
cut-off
value
determined
purified
gelatinases
of
supernatant
HCT
116
cell
line.
by
preparative
native-polyacrylamide
gel
electrophoresis
based
on
charge
size
proteins.
Furthermore,
quality
control
(QC)
results
performed
following
standard
densitometry
methods.
Results:
A
major
epitope
can
trigger
immune
system
scavenge
for
protein
from
line
commercially
available,
full-length
estimated
at
30
μg/mL.
QC
indicated
specificity
80%,
96.7%,
accuracy
91%,
precision
91.66%.
area
under
curve
0.827
(
P
<
0.001).
positive
predictive
83%,
negative
96%.
Conclusions:
synthetic
detect
Native
considered
main
biomarker
immunoassay
POCT
diagnose
dry
eye
disease
(DED).
In
accordance
with
results,
point
care
test
be
utilized
screening
patients
suffering
DED.
ACS Nano,
Journal Year:
2023,
Volume and Issue:
17(24), P. 25118 - 25135
Published: Dec. 5, 2023
Dry
eye
(DE)
disease,
which
is
primarily
linked
to
aqueous
deficiency,
an
escalating
health
issue
worldwide,
mainly
due
the
widespread
use
of
electronic
devices.
The
major
obstacles
in
DE
pharmacotherapy
include
insufficient
therapeutic
efficacy
and
low
ocular
bioavailability.
This
study
presents
development
a
ceria-based
nanosystem
carry
gabapentin
(GBT),
aiming
offer
comprehensive
relief
from
symptoms.
We
prepared
multifunctional
nanoceria
capped
with
thiolated
gelatin
followed
by
cross-linking
glutaraldehyde,
yielding
nanocarrier
desirable
biocompatibility
antioxidant,
anti-inflammatory,
antiangiogenic,
antiapoptotic,
neuronal
protective
activities.
Specifically,
highly
abundant
thiol
groups
on
increased
cellular
uptake
2.3-fold
its
mucin-binding
efficiency
10-fold,
thereby
extending
retention
amplifying
activity.
Moderate
not
only
enhanced
bioavailability
but
also
provided
slow,
degradation-controlled
release
GBT
promote
lacrimal
stimulation
restore
tear
film.
In
rabbit
model
DE,
topical
administration
our
GBT/nanoceria
nanoformulation
resulted
alleviation
symptoms,
including
repairing
corneal
epithelial
damage,
preserving
nerve
density,
stimulating
secretion,
demonstrating
superior
performance
comparison
free
drug.
These
results
underscore
safety
potential
this
innovative
for
pharmacotherapy.
Pharmaceutics,
Journal Year:
2023,
Volume and Issue:
15(4), P. 1094 - 1094
Published: March 29, 2023
Posterior
segment
eye
diseases
present
a
challenge
in
treatment
due
to
the
complex
structures
that
serve
as
robust
static
and
dynamic
barriers,
limiting
penetration,
residence
time,
bioavailability
of
topical
intraocular
medications.
This
hinders
effective
requires
frequent
dosing,
such
regular
use
drops
or
visits
ophthalmologist
for
intravitreal
injections,
manage
disease.
Moreover,
drugs
must
be
biodegradable
minimize
toxicity
adverse
reactions,
well
small
enough
not
affect
visual
axis.
The
development
nano-based
drug
delivery
systems
(DDSs)
can
solution
these
challenges.
First,
they
stay
ocular
tissues
longer
periods
reducing
frequency
administration.
Second,
pass
through
offering
higher
targeted
are
otherwise
inaccessible.
Third,
made
up
polymers
nanosized.
Hence,
therapeutic
innovations
nanosized
DDS
have
been
widely
explored
ophthalmic
applications.
In
this
review,
we
will
concise
overview
DDSs
utilized
diseases.
We
then
examine
current
challenges
faced
management
posterior
explore
how
various
types
nanocarriers
enhance
our
arsenal.
A
literature
review
pre-clinical
clinical
studies
published
between
2017
2023
was
conducted.
Through
advances
materials,
combined
with
better
understanding
pharmacology,
rapidly
evolved,
showing
great
promise
overcome
currently
encountered
by
clinicians.
Biomolecules,
Journal Year:
2024,
Volume and Issue:
14(1), P. 80 - 80
Published: Jan. 8, 2024
Sjögren’s
syndrome
dry
eye
(SSDE)
is
a
subset
of
marked
by
symptoms
that
distinct
from
non-Sjögren’s
(NSSDE).
As
SSDE
can
lead
to
severe
complications,
its
early
detection
imperative.
However,
the
differentiation
between
and
NSSDE
remains
challenging
due
overlapping
clinical
manifestations.
This
review
endeavors
give
concise
overview
classification,
pathophysiology,
features
presentation,
ocular
systemic
diagnosis,
management
SSDE.
Despite
advancements,
limitations
in
current
diagnostic
methods
underscore
need
for
novel
modalities.
Thus,
examines
various
biomarkers
utilized
identification,
encompassing
serum,
salivary,
tear
analyses.
Recent
advancements
proteomic
research
exosomal
offer
promising
potential.
Through
comprehensive
literature
spanning
2016
2023,
we
highlight
molecular
insights
advanced
modalities
have
potential
enhance
our
understanding
diagnosis
Journal of Clinical Medicine,
Journal Year:
2025,
Volume and Issue:
14(2), P. 568 - 568
Published: Jan. 17, 2025
Rheumatoid
arthritis
(RA)
is
an
immune-mediated
disease
characterized
by
polyarthritis
that
affects
the
small
joints
of
bilateral
upper
and
lower
extremities.
RA
shares
several
common
clinical
symptoms
with
Sjögren’s
syndrome
(SS),
another
rheumatic
caused
lymphocytic
infiltration
exocrine
glands,
dry
eye
mouth
being
two
most
symptoms.
Anti-Ro/SS-A
antibodies,
a
diagnostic
biomarker
SS,
are
positive
in
patients
at
certain
rate.
The
coexistence
SS
and/or
positivity
for
anti-Ro/SS-A
antibodies
influences
activity
effectiveness
classes
disease-modifying
antirheumatic
drugs
(DMARDs).
Furthermore,
RA,
DMARDs,
including
methotrexate,
associated
onset
lymphoproliferative
disorders
(LPD).
In
contrast,
biological
such
as
tocilizumab
rituximab,
plausible
options
without
risk
LPD
relapse.
Considering
results
studies
introduced
this
article,
could
be
considered
phenotype
different
from
isolated
perspective
refractoriness
to
DMARD
therapy
risk.
Hence,
rheumatologists
should
observe
caution
when
choosing
DMARDs.
Further
needed
establish
appropriate
treatment
presence
antibodies.
Frontiers in Immunology,
Journal Year:
2025,
Volume and Issue:
15
Published: Jan. 28, 2025
Background
Sjögren’s
Disease
(SjD)
is
an
autoimmune
disease
characterized
by
lymphocytic
infiltration
of
salivary
and
lacrimal
glands
(LG).
The
LG
produces
the
protein-rich
aqueous
component
tears,
SjD-associated
dacryoadenitis
(AD)
may
thus
alter
tear
autoantibody
composition.
Methods
presence
tertiary
lymphoid
structures
(TLS)
in
from
two
murine
models
AD,
male
non-obese
diabetic
(NOD)
insulitis
resistant
(NOR)
mice,
were
evaluated
using
immunofluorescence.
IgG
IgA
reactivity
serum
tears
these
probed
three
studies
against
a
panel
80-120
autoantigens
microarrays
relative
to
healthy
BALB/c
mice.
Sources
Ig
investigated
scRNA-Seq
(GSE132420).
Data
analyzed
R
package
Limma
Seurat.
Results
Analysis
immunofluorescence
sections
both
SjD
showed
TLS.
Only
one
was
significantly
elevated
across
all
studies.
Three
autoantibodies
but
not
Conversely,
six
thirteen
(6
sharing
same
autoantigen)
models.
Igha
Ighg2b
expressing
cells
identified
plasma
cell
cluster
NOD.H2b
LG.
Conclusion
NOD
NOR
mice
with
AD
have
distinct
profiles
serum.
Tear
isotype
greater
diversity
than
autoantibodies.
TLS
observed
are
likely
source
Journal of Clinical Medicine,
Journal Year:
2024,
Volume and Issue:
13(6), P. 1777 - 1777
Published: March 20, 2024
Dry
eye
disease
(DED)
is
a
continuing
medical
challenge,
further
worsened
in
the
autoimmune
inflammatory
hyperactivation
milieu
of
Sjögren's
syndrome
(SS)
due
to
disturbances
innate
and
adaptive
immunity
with
malfunctioning
neuro-endocrine
control.
However,
pathogenetic
mechanisms
SS
DED
are
not
fully
established.
This
review
summarized
available
evidence,
from
systematic
reviews,
meta-analyses,
randomized
clinical
trials,
for
efficacy
safety
ocular
therapeutics
management
DED.
Relevant
studies
were
obtained
major
databases
using
appropriate
keywords.
The
largely
empirical
symptomatic,
supportive,
restorative
treatments
have
significant
limitations
as
they
do
alter
local
systemic
progression.
Topical
therapies
expanded
include
biologics,
surgical
approaches,
scleral
lens
fitting,
lid
margin
disease,
treatments,
nutritional
support,
transplantation
stem
cells.
They
curative,
cannot
permanently
restore
surface's
homeostasis.
These
approaches
efficacious
short
term
most
studies,
more
variability
outcome
measures
among
long
term.
offers
an
interdisciplinary
perspective
that
enriches
our
understanding
updated
addresses
current
knowledge
gaps
identifies
promising
areas
future
research
overcome
this
challenge.
Journal of Proteome Research,
Journal Year:
2024,
Volume and Issue:
23(6), P. 2219 - 2229
Published: April 29, 2024
Sjögren's
syndrome
(SS)
is
an
autoimmune
rheumatic
disorder
characterized
by
exocrine
gland
dysfunction,
mainly
from
the
lacrimal
and
salivary
glands.
The
disease
causes
severe
aqueous
dry
eye
(DED)
associated
with
high
rates
of
complications,
including
corneal
ulceration,
scaring,
perforation.
Systemic
complications
may
occur
as
well
a
higher
risk
developing
lymphoma.
Diagnosis
SS-DED
often
delayed
difficult
to
establish.
With
aim
discovering
biomarkers
help
discriminate
patients,
combination
untargeted
targeted
LC-MS/MS
analyses
were
performed
on
tear
samples
collected
Schirmer
strips
subjected
tryptic
digestion.
Following
analysis
three
cohorts
development
two
LC-sMRM
methods
for
verification
putative
found
in
first
cohort
samples,
64
proteins
could
be
linked
syndrome,
hopes
helping
confirm
diagnoses
potentially
stratifying
severity
these
patients.
Proteins
that
increased
showed
activation
immune
system
alterations
homeostasis.
Several
proteases
protease
inhibitors
significantly
changing
SS-DED,
consistent
decrease
specific
known
secreted
gland.
Ophthalmology Science,
Journal Year:
2024,
Volume and Issue:
4(5), P. 100546 - 100546
Published: May 7, 2024
This
study
aims
to
develop
and
assess
an
infrastructure
using
Python-based
deep
learning
code
for
future
diagnostic
management
purposes
related
dry
eye
disease
(DED)
utilizing
smartphone
images.
Current Issues in Molecular Biology,
Journal Year:
2023,
Volume and Issue:
45(4), P. 2881 - 2894
Published: April 1, 2023
Ocular
diseases
have
a
strong
impact
on
individuals,
the
effects
of
which
extend
from
milder
visual
impairment
to
blindness.
Due
this
and
their
prevalence,
these
conditions
constitute
important
health,
social
economic
challenges.
Thus,
improvements
in
early
detection
diagnosis
will
help
dampen
conditions,
both
patients
healthcare
systems
alike.
In
sense,
identifying
tear
biomarkers
could
establish
better
non-invasive
approaches
diagnose
monitor
responses
therapy.
With
mind,
we
developed
solid
phase
capture
assay,
based
antibody
microarrays,
quantify
S100A6,
MMP-9
CST4
human
samples,
used
arrays
study
samples
healthy
controls
with
Sjögren’s
Syndrome,
at
times
concomitant
rheumatoid
arthritis.
Our
results
point
out
that
S100A6
seems
be
positively
correlated
arthritis,
consistent
systemic
nature
autoinflammatory
pathology.
provide
evidence
microarrays
may
potentially
certain
pathologies,
possibly
paving
way
for
significant
future
care
patients.