focus on Sjogren’s Dry Eye Disease - pathogenesis, patient management, and new advancements in therapy

Journal of Education Health and Sport, Journal Year: 2023, Volume and Issue: 37(1), P. 128 - 139

Published: Aug. 8, 2023

Introduction: Sjogren’s syndrome (SS) is a systemic chronic inflammatory autoimmune disease of unknown etiology that primarily affects exocrine glands. Most commonly presents with dryness the mouth and eyes, but it can also affect major organs systems body increase risk non-Hodgkin lymphoma. Due to non-specific symptoms pose diagnostic challenges lack standardized screening tools classification criteria, global incidence prevalence are hard evaluate. Studies estimate between 400,000 3.1 million adults worldwide most likely condition significantly underdiagnosed. Aim study: Review current knowledge about dry eye disease, underlying pathologies, problems, treatment options focus on recent advancements in therapy. Methods materials: A review chosen literature PubMed database, MDPI GoogleScholar years 2000-2023 was conducted using following keywords: “Sjogren’s syndrome”, ophthalmology”, associated eye”, “Lymphoma “Diagnosing epidemiology”. Results: far from rare many lower quality life mortality morbidity. its surreptitious symptom onset, New modalities for horizon. Conclusions: Standardized, internationally recognized criteria high level clinical suspiciousness needed timely accurate diagnosis syndrome. Quick significant facilitating factor maintaining eye.

Language: Английский

The ability of antibody against truncated matrix metalloproteinase 9 peptide to evaluate the native protein on tear drops of dry eye disease patients by a point-of-care diagnostic system

Journal of Current Ophthalmology, Journal Year: 2023, Volume and Issue: 35(2), P. 170 - 176

Published: April 1, 2023

Purpose: To obtain a reactive and specific antibody against truncated matrix metalloproteinase 9 (MMP-9), that has reactivity toward the native protein. Precision, accuracy, specificity, sensitivity were evaluated using point-of-care test. Methods: An in silico study was used to confirm anti peptide MMP-9 is MMP-9. After an titer assessment, purification, characterization, assessed. The cut-off value determined purified gelatinases of supernatant HCT 116 cell line. by preparative native-polyacrylamide gel electrophoresis based on charge size proteins. Furthermore, quality control (QC) results performed following standard densitometry methods. Results: A major epitope can trigger immune system scavenge for protein from line commercially available, full-length estimated at 30 μg/mL. QC indicated specificity 80%, 96.7%, accuracy 91%, precision 91.66%. area under curve 0.827 ( P < 0.001). positive predictive 83%, negative 96%. Conclusions: synthetic detect Native considered main biomarker immunoassay POCT diagnose dry eye disease (DED). In accordance with results, point care test be utilized screening patients suffering DED.

Language: Английский