Smoking and High‐Altitude Exposure Affect Intrinsic Neural Activity: A fMRI Study of Interactive Effects DOI Creative Commons
Qingqing Lv, Minghe Wang,

Chunxiao Bu

et al.

Addiction Biology, Journal Year: 2025, Volume and Issue: 30(4)

Published: April 1, 2025

ABSTRACT Smoking and high‐altitude (HA) exposure both adversely affect human health, with smoking linked to various cancers environments causing physiological neurological changes. Although the effects of HA on brain structure function have been studied separately, their combined impact is still rarely explored. This study aims investigate interactive intrinsic activity using resting‐state functional magnetic resonance imaging (rs‐fMRI) analysed by amplitude low‐frequency fluctuations (ALFF) method. We used a mixed sample design, including four groups: (i) smokers ( n = 22); (ii) nonsmokers (iii) sea‐level (SL) 26); (iv) SL 26), for total 96 male participants. All subjects underwent imaging. ALFF was assess differences in among groups. Two‐way analysis variance (ANOVA) conducted analyse smoking, interaction ALFF. As main effect elevated found right superior frontal gyrus, middle inferior cingulate cortex precentral gyrus. exposure, putamen, insula, temporal gyrus fusiform. A significant between observed Post hoc showed significantly increased groups versus smokers; nonsmokers; nonsmokers. Our findings demonstrate that independently influence spontaneous activity, two factors modulating function. These results offer neuroimaging‐based perspective substance addiction populations contribute deeper understanding adaptation.

Language: Английский

Characteristics of transcriptome and chromatin accessibility in the peripheral blood after acute hypoxia exposure DOI Creative Commons

Kuo Zeng,

Ping Yuan,

Jin‐feng Xuan

et al.

BMC Biology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 21, 2025

Human responses and acclimation to the environmental stresses of high altitude low oxygen are multifaceted regulated by multiple genes. However, mechanism how body adjusts in a low-oxygen environment is not yet clear. Hence, we performed RNA sequencing (RNA-seq) ATAC (ATAC-seq) observe changes transcriptome chromatin accessibility peripheral blood eight individuals at 1 h post adaptation simulated plateau with 3500 m 4500 altitude, respectively. Differential expression analysis Boruta algorithm identified differentially expressed genes (DEGs) accessible regions (DARs) associated hypoxia adaptation. Specifically, RNA-seq 93 7 DEGs after 45 8 m. Additionally, ATAC-seq screened 12 4 DARs adaption 15 5 adaption. Moreover, combined revealed that 10 hub were independently from protein–protein interaction (PPI) network for each altitude. Gene enrichment displayed most related pathways. Our results can provide reference early response organism hypoxic

Language: Английский

Citations

0
Enhancing Oxygen Utilization and Mitigating Oxidative Stress in Tibetan Chickens for Adaptation to High-Altitude Hypoxia DOI Creative Commons
Ruyi Hao,

Xianpei Ao,

Yijing Xu

et al.

Poultry Science, Journal Year: 2025, Volume and Issue: 104(4), P. 104893 - 104893

Published: Feb. 7, 2025

Tibetan chicken (TBC) is one of the native poultry species that well adapted to high-altitude environment Qinghai-Tibet Plateau. To elucidate genetic mechanisms underlying adaptation, transcriptomes five tissues (heart (HE), lung (LU), liver (LI), ovary (OV), and abdominal fat (AB)) were compared between TBCs Roman chickens (RMCs) inhabiting plateau for year. Moreover, weighted gene co-expression network analysis (WGCNA) was applied detect tissue-associated modules hub genes. A total 1105, 239, 400, 483, 275 differentially expressed genes (DEGs) identified in LI, HE, LU, AB, OV tissues, respectively. Fifteen tissue-specific TBC thirteen RMC. Analysis transcription factor (TF) binding sites revealed nineteen TFs twenty RMC across pool these two breeds. Functional enrichment analyses demonstrated exhibited robust capacity oxygen transport, heme binding, oxidative phosphorylation, antioxidant responses regions. Further investigation function indicated involvement ATF4, CEBPA, TCF7L1, GFI1B improving transport TBCs. These associated with angiogenesis or hematopoiesis likely linked various regulatory functions facilitate communication multiple tissues. In conclusion, have developed a systemic adaptive mechanism cope high altitudes, involving coordinated transcriptional regulation multi-tissues enhance utilization, along amelioration stress.

Language: Английский

Citations

0
Unlocking the biotechnological potential of Decalepis arayalpathra: exploring synthetic seed production, metabolic profiling, genetic stability, and the impact of photosynthetic photon flux density on acclimatization DOI Creative Commons

Zishan Ahmad,

Olga Babich, Станислав Сухих

et al.

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 13, 2025

Decalepis arayapathra is an important medicinal plant known for several values, however, due to overharvesting, habitat destruction, and its limited geographical distribution, D. faces severe threats of extinction. A synthetic seed protocol was developed this plant, representing a novel approach in propagation conservation. Nodal segments (NS) were encapsulated sodium alginate (SA) matrix. 3% SA with 100 mM CaCl2 solutions best obtain ideal beads fine texture. Murashige Skoog (MS) medium consisting BA 5.0 µM + NAA 0.5 ADS 20.0 resulted maximum regrowth frequency 71.26% 3.13 shoots per bead shoot length 4.10 cm after six weeks culture. Rooting the microshoots better observed half- strength MS 2.5 NAA, resulting 3.1 roots microshoot root 3.0 four culture, followed by successful acclimatization. The study investigated effect photosynthetic photon flux density (PPFD) levels 50 300 PPFD on various physiological biochemical parameters during acclimatization vitro-derived plants. Results showed increase pigments, including chlorophyll carotenoids, as well enhanced net rate (PN) stomatal conductance (gs) prolonged acclimatization, higher being more effective. Antioxidant enzyme activities, SOD, CAT, APX, GR, increased over time, except which began decline 21 days under both light conditions. Stress markers such malondialdehyde (MDA) electrolyte leakage decreased indicating Genetic fidelity confirmed through clear monomorphic banding patterns obtained using RAPD ISSR markers. Quantification 2H4MB (2-hydroxy-4-methoxy benzaldehyde) synseed-derived HPLC revealed concentration 16.27 µg/ml. Metabolic profiling tuber GC-MS identified major minor metabolites. This offers breakthrough conservation technology, enabling sustainable while preserving genetic stability. It ensures consistent supply bioactive compound 2H4MB, promoting research commercial applications.

Language: Английский

Citations

0
Improved oxygen saturation and acclimatization with bacteriotherapy at high altitude DOI Creative Commons
James J. Yu, Esteban A. Moya, Hunter Cheng

et al.

iScience, Journal Year: 2025, Volume and Issue: 28(4), P. 112053 - 112053

Published: Feb. 17, 2025

Language: Английский

Citations

0
Proteomics and Expression of HIF2α/BNIP3L Signaling in Yak Brains at Different Altitudes DOI Open Access
Qian Zhang, Yan Cui, Sijiu Yu

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(4), P. 1675 - 1675

Published: Feb. 16, 2025

The yak, a unique inhabitant of low-oxygen environments, exhibits brain adaptability to hypoxic conditions. However, the impact hypoxia on yak proteomics and expression HIF2α/BNIP3L signaling pathway remains unexplored. This study utilized TMT-based analysis identify differentially expressed proteins (DEPs) in cerebral cortexes 9-month-old yaks at high (n = 3) low altitudes. Additionally, qRT-PCR, Western blot, immunohistochemistry, immunofluorescence were used analyze HIF2α, BNIP3L, Beclin1, LC3-II, cleaved caspase-3 various regions from both altitude groups. KEGG revealed that DEPs mainly concentrated synthesis metabolism, DNA replication, repair pathways. Specifically, autophagy attracted our attention due its absence other animals. LC3-II increased significantly. Furthermore, results qRT-PCR blot showed that, same altitude, mRNA protein levels Beclin1 hippocampi significantly higher than those thalami, medulla oblongatae, cerebella (p < 0.05), while was not different among > 0.05). within region, high-altitude low-altitude yaks. Moreover, there no difference between Immunohistochemistry HIF2α-positive nucleus cytoplasm neurons, cytoplasm. caspase-3, NeuN co-located neurons cortex, hippocampus, thalamus, oblongata, cerebellum, respectively. offers complete characterization cortex proteome indicates these are more resistant hypoxia. In addition, may enhance tissue adaptation

Language: Английский

Citations

0
Metabolomic analysis of rat arterial serum under hypobaric hypoxia: Adaptive regulation of physiological systems by metabolic reprogramming DOI Creative Commons

Drosdowsky Ma,

Bing Li,

Baozhong Xin

et al.

Biochemistry and Biophysics Reports, Journal Year: 2025, Volume and Issue: 41, P. 101943 - 101943

Published: Feb. 18, 2025

Language: Английский

Citations

0
Elucidating the potential role of quercetin in augmenting the acclimatization process via controlling high altitude induced redox imbalance in rats: a molecular approach DOI

Vaishnavi Rathi,

Sarada S.K. Sagi, Amit Kumar Yadav

et al.

Pharmacological Research - Natural Products, Journal Year: 2025, Volume and Issue: unknown, P. 100187 - 100187

Published: Feb. 1, 2025

Language: Английский

Citations

0
Cardiorespiratory fitness and effects of ubiquinol during high-altitude acclimatization and deacclimatization: the SCARF trial DOI Creative Commons
Hailin Lv, Zhen Liu, Mengjia Sun

et al.

iScience, Journal Year: 2025, Volume and Issue: 28(3), P. 112112 - 112112

Published: Feb. 27, 2025

Language: Английский

Citations

0
Comparison of the Predictive Capacity of Oxygenation Parameters, Oxygenation Indices, and CURB‐65 to Mortality, Mechanical Ventilation, and Vasopressor Support in Community‐Acquired Pneumonia at Different Altitudes DOI Creative Commons
Eduardo Tuta-Quintero, Alirio Bastidas,

Angélica Mora

et al.

Canadian Respiratory Journal, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

Background: Populations residing at high altitudes display distinct physiological adaptations that are essential for understanding respiratory diseases. However, there is limited research on how these affect the assessment and prognosis of community-acquired pneumonia (CAP). Methods: A prognostic validation nested within a retrospective cohort was conducted subjects with admitted to two high-complexity institutions in Colombia different above sea level. The receiver operating characteristic (ROC) curves were calculated SaO2, PaO2, SpO2, A-a O2 gradient, a-A index, PaO2/FiO2, SpO2/FiO2, CURB-65 score predict 30-day mortality, requirement invasive mechanical ventilation (IMV), need vasopressor support. Results: 3467 selected analysis, 73.7% (2557/3467) 26.2% (910/3467) low altitudes. ≥ 2 showed performance predicting mortality 0.707 (95% CI: 0.653-0.761; p < 0.001) 0.737 0.709-0.765; PaO2/FiO2 ≤ 300 IMV support 0.734 0.685-0.783; 0.724 (0.674-0.775; altitudes, respectively. SpO2/FiO2 350 0.679 (0.507-0.85; gradient 10 0.686 0.537-0.835; p=0.06) Conclusion: In patients CAP 2500 m level, show greater predictive capacity IMV, requirements. good performance.

Language: Английский

Citations

0
Oxygen metabolism abnormalities and high-altitude cerebral edema DOI Creative Commons
Zhi Li, Jianping Zhang, Xiaoxia Zhang

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 19, 2025

Hypobaric hypoxia is widely recognized as a prominent risk factor for high-altitude cerebral edema (HACE), which contributes to the exacerbation of multiple pathological mechanisms, including oxidative stress, mitochondrial dysfunction, disruption blood−;brain barrier integrity, neuroinflammation, and neuronal apoptosis. Among these abnormalities in oxygen metabolism, hypoxia, play pivotal roles pathophysiology HACE. In this review, our objective enhance comprehension underlying molecular mechanisms implicated HACE by investigating potential involvement metabolism. Addressing aberrations metabolism holds promise providing innovative therapeutic strategies managing

Language: Английский

Citations

0